Genetics Final Exam: Handpicked Questions
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Which of the following statements is correct?
A) Histone acetylation opens up chromatin.
B) DNA methylation represses gene expression
C) DNA methylation results in condensation of chromatin
D) Deacetylation reduces gene expression
E) All of the above - correct answer- -E) All of the above
How does tryptophan, the end product of the trp operon,
function in the regulation of the operon? #
A) It binds to the repressor preventing it from binding to
DNA thus allowing transcription of the operon.
B) It binds to the repressor and only then can the repressor
bind to DNA allowing transcription of the operon.
C) It binds directly to DNA and inhibits transcription of
the operon.
D) It binds to the repressor and only then can this
complex bind to DNA preventing transcription of the
operon - correct answer- -D) It binds to the repressor and only then can this
complex bind to DNA preventing transcription of the
operon
What is the function of an antibiotic resistance gene in
,a vector?
A) To allow resistant transformants to grow on
selective medium
B) To distinguish introns from exons
C) To allow viral replication
D) To screen for vectors with inserts
E) To allow plasmid replication - correct answer- -A) To allow resistant transformants to
grow on
selective medium
If you want to introduce and express a protein from a human gene
in bacterial cells, which of the following change(s) you need to
make in the human gene?
A. Replace its promoter with a bacterial promoter
B. Add a bacterial transcription terminator at the end of its coding
region
C. Add a ribosome binding site (Shine-Dalgarno box) near the
initiation codon
D. Remove introns
E. All of the above - correct answer- -E. All of the above
A Mutant that does not produce lacI shows
A) no expression lac operon
B) expression of lac operon only in the presence
of lactose
C) expression of lac operon in the presence or
absence of lactose
, D) expression of lac operon only in the absence of
lactose - correct answer- -C) expression of lac operon in the presence or
absence of lactose
In a strain of E.coli with a genotype I+ O+ Z+ Y+ / I− Oc
Z+ Y−
the expression of galactosidase and permease is _______ and
__________, respectively
A) Inducible, constitutive
B) constitutive, inducible
C) constitutive, constitutive
D) inducible, inducible
E) inducible, no expression - correct answer- -B) constitutive, inducible
The genotype of an E.coli strain is R+ P+ O− att+ trpE+ trpC− /
R− P+ O+ att+ trpE− trpC+. Predict the expression of trpE.
A) constitutive but attenuated
B) constitutive and not attenuated
C) induced by tryptophan but attenuated
D) repressed by tryptophan
E) Not expressed in the presence or absence of tryptophan - correct answer- -A)
constitutive but attenuated
If there is a mutation in GAL80-
what happens to expression of
Gal7, Gal10 and Gal1 genes
from Elite Universities - Certified for Academic
Excellence from World-Leading Institutions
Which of the following statements is correct?
A) Histone acetylation opens up chromatin.
B) DNA methylation represses gene expression
C) DNA methylation results in condensation of chromatin
D) Deacetylation reduces gene expression
E) All of the above - correct answer- -E) All of the above
How does tryptophan, the end product of the trp operon,
function in the regulation of the operon? #
A) It binds to the repressor preventing it from binding to
DNA thus allowing transcription of the operon.
B) It binds to the repressor and only then can the repressor
bind to DNA allowing transcription of the operon.
C) It binds directly to DNA and inhibits transcription of
the operon.
D) It binds to the repressor and only then can this
complex bind to DNA preventing transcription of the
operon - correct answer- -D) It binds to the repressor and only then can this
complex bind to DNA preventing transcription of the
operon
What is the function of an antibiotic resistance gene in
,a vector?
A) To allow resistant transformants to grow on
selective medium
B) To distinguish introns from exons
C) To allow viral replication
D) To screen for vectors with inserts
E) To allow plasmid replication - correct answer- -A) To allow resistant transformants to
grow on
selective medium
If you want to introduce and express a protein from a human gene
in bacterial cells, which of the following change(s) you need to
make in the human gene?
A. Replace its promoter with a bacterial promoter
B. Add a bacterial transcription terminator at the end of its coding
region
C. Add a ribosome binding site (Shine-Dalgarno box) near the
initiation codon
D. Remove introns
E. All of the above - correct answer- -E. All of the above
A Mutant that does not produce lacI shows
A) no expression lac operon
B) expression of lac operon only in the presence
of lactose
C) expression of lac operon in the presence or
absence of lactose
, D) expression of lac operon only in the absence of
lactose - correct answer- -C) expression of lac operon in the presence or
absence of lactose
In a strain of E.coli with a genotype I+ O+ Z+ Y+ / I− Oc
Z+ Y−
the expression of galactosidase and permease is _______ and
__________, respectively
A) Inducible, constitutive
B) constitutive, inducible
C) constitutive, constitutive
D) inducible, inducible
E) inducible, no expression - correct answer- -B) constitutive, inducible
The genotype of an E.coli strain is R+ P+ O− att+ trpE+ trpC− /
R− P+ O+ att+ trpE− trpC+. Predict the expression of trpE.
A) constitutive but attenuated
B) constitutive and not attenuated
C) induced by tryptophan but attenuated
D) repressed by tryptophan
E) Not expressed in the presence or absence of tryptophan - correct answer- -A)
constitutive but attenuated
If there is a mutation in GAL80-
what happens to expression of
Gal7, Gal10 and Gal1 genes
