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BLOOD BANK TEST Actual Questions & Answers 2025/2026

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BLOOD BANK TEST Actual Questions & Answers 2025/2026 Question Answer kLIEHAUER-BETKE STAIN ACID ELUTION IS THE METHOD USED MOST FREQUEUNTLY TO QUANTIFY THE NUMBER OF FETAL CELLS IN THE MATERNAL CIRCULATION.THIS METHOD IS BASED ON THE FACT THAT FETAL HEMOGLOBIN IS RESISTANT TO ACID ELUTION AND ADULT HEMOGLOBIN IS NOT. 1. List the most common to least common causes of HDFN as discusses in class. ANY IgG ANTIBODY IS CAPABLE OF CAUSING HDFN IF THE FETAL RED CELLS POSSESS ANTIGENS THAT THE MOTHER LACKS, ANTI-c SECOND MOST COMMON CAUSE, CAN BE IN COMBINATION WITH ANTI-D-NEXT IN ANTI-K 2. Know the significance of the optical densities given in class for the Liley method for testing amniotic fluid. APIRATED FLOUID IS SCANNED ON A SPECTOPHOTMETER FROM 350 TO 700 nm. THE CHANGE IN OPTICAL DENSITY ABOVE THE BASELINE 450 nm IS PLOTTED ON THE LILEY GRAPH

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BLOOD BANK TEST
Actual Questions & Answers
2025/2026
Question Answer


ACID ELUTION IS THE METHOD USED MOST
FREQUEUNTLY TO QUANTIFY THE NUMBER OF
FETAL CELLS IN THE MATERNAL
kLIEHAUER-BETKE STAIN CIRCULATION.THIS METHOD IS BASED ON THE
FACT THAT FETAL HEMOGLOBIN IS RESISTANT
TO ACID ELUTION AND ADULT HEMOGLOBIN
IS NOT.


ANY IgG ANTIBODY IS CAPABLE OF CAUSING
1. List the most common to HDFN IF THE FETAL RED CELLS POSSESS
least common causes of ANTIGENS THAT THE MOTHER LACKS, ANTI-c
HDFN as discusses in class. SECOND MOST COMMON CAUSE, CAN BE IN
COMBINATION WITH ANTI-D-NEXT IN ANTI-K


2. Know the significance of APIRATED FLOUID IS SCANNED ON A
the optical densities given in SPECTOPHOTMETER FROM 350 TO 700 nm.
class for the Liley method for THE CHANGE IN OPTICAL DENSITY ABOVE THE
testing amniotic fluid. BASELINE 450 nm IS PLOTTED ON THE LILEY

, GRAPH


MOST FREQUENTLU USED METHOD FOR
SCREENING FMH IS THE ROSETTE TEST.THIS
3fetal screen and Kliehauer-
TEST USES THE MATERNAL CELLS AND IS
Betke stain. What do each
INCUBATED WITH ANTI-D. THEN WASHED,
look like if positive? What
INDICATOR D-POS CELLS ARE ADDED,WHICH
cells are involved?
BIND TO THE ANTI-D TO FORM ROSETTES
AROUND THE D-POS FETAL CELLS


4. What color do the fetal
FETAL CELLS STAIN PINK AND MATERNAL
cells stain? What color do
CELLS APPEAR "GHOST" LIKE
the maternal cells stain?


5. Know the graft-vs-host
disease is prevented what
LEUKOCYTE REDUCED RED BLOOD CELLS
kind of treatment to the
RBC?


ESTIMATE VOLUME OF FETAL BLOOD IN
6. KNOW HOW TO
MATERNAL CIRCULATIONDIVIDE mL OF FETAL
CALCULATE THE NUMBER OF
BLOOD BY 30; EACH 300 UG DOSE OF RhIG
VIALS TO GIVE TO A WOMEN
PROTECTS AGAINST 30mL OF WHOLE
WHO HAS HAD A
BLOODROUND THE CALCULATED DOESE UP IF
FETOMATERNAL BLEED. See
5 FOLLOWING DECIMAL POINT OR DOWN IF
page 295
LESS THAN 5 ADD 1 VIAL OF RhIG TO


7. Why do we perform an CORRECTS ANEMIA WITHOUT EXPANDING
exchange transfusion? List BLOOD VOLUMEREMOVES A SENSITIZED
the three main reasons. NEWBORN'S RED CELLS AND REPPLACES

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