SEROLOGY EXAM 1 STUFF || WITH COMPLETE
SOLUTIONS.
Cells that are precursors of plasma cells and also produce immunoglobulins are: correct answers
B lymphocytes
Monoclonal antibodies are produced by correct answers Hybridomas
The portion of an antigen that binds to an antibody or T cell receptor is called a(n) correct
answers Epitope
In immunofixation electrophoresis: correct answers The antigen is electrophoresed and then
monospecific antisera is reacted with it
Which of the following are L chains of antibody molecules? correct answers Kappa
Which immunoglobulin appears first in the primary immune response? correct answers IgM
Which antibody provides protection to the growing fetus because it is able to cross the placenta?
correct answers IgG
A substrate is first exposed to a patient's serum, then after washing, anti-human immunoglobulin
labeled with a fluorochrome is added. The procedure described is: correct answers indirect
fluorescence
In the enzyme- linked immunosorbent assay (ELISA) the visible reaction is due to a reaction
between correct answers Enzyme and substrate
Identical antibodies produced from a single clone of the plasma cells describes correct answers
Monoclonal antibodies
The detection of precipitation reactions depends on the presence of optimal proportions of
antigen and antibody. A patient's sample contains a large amount of antibody, but the reaction in
a test system containing antigen is negative. What has happened? correct answers Prozone
phenomenon
A Fab fragment consists of correct answers one L chain and one half of an H chain
Antibodies are produced by: correct answers B cells
Which of the following immunoglobin is the most efficient at agglutination: correct answers IgM
Which statement best describes flow-through immunoassays (immunochromatography-like a
pregnancy test from CVS)? correct answers They are designed for point of care testing
, Which test has the greatest sensitivity for antigen detection? correct answers ELISA
Which one of the following describes direct immunofluorescence array? correct answers
Conjugated reagent antibody reacts with antigen to form antigen-antibody complexes
Which immunoglobulin can cross the placenta? correct answers IgG
Which of the following immunoglobulins is present in the highest concentration in normal
human serum? correct answers IgG
A laboratory is evaluating an enzyme-linked immunosorbent assay (ELISA) for detecting an
antibody to cyclic citrullinated peptide (CCP), which is a marker for rheumatoid arthritis. The
laboratory includes serum from healthy volunteers and patients with other connective tissue
diseases in the evaluation. These specimens determine which factor of the assay? correct answers
Specificity
How does the secondary humoral immune response differ from the primary response? correct
answers The antibody levels produced are higher in the secondary immune response
What comprises the indicator system in an indirect ELISA for detecting antibody? correct
answers Enzyme-conjugated antibody + chromogenic substrate
The major class of immunoglobin found in adult human serum is correct answers IgG
After exposure to antigen, the first antibodies that can be detected belong to correct answers IgM
In hybridoma technology, the desirable fused cell is the: correct answers myeloma- lymphocyte
hybrid
Which class of immunoglobulin possesses delta heavy chains? correct answers IgD
Measurement of serum levels of which of the following immunoglobulins can serve as a
screening test for multiple allergies/parasitic infections? correct answers IgE
Blood is drawn from a patient for serological tests for a viral disease at the time of onset and
again 4 weeks later. The results of the test are considered diagnostic if the: correct answers
second antibody titer is at least 4x the first
Antibodies composed of IgG immunoglobin: correct answers can cross the placenta from mother
to fetus
The IgM molecule is a: correct answers pentamer
Immunoassays are based on the principle of: correct answers antibody recognition of a specific
antigen
SOLUTIONS.
Cells that are precursors of plasma cells and also produce immunoglobulins are: correct answers
B lymphocytes
Monoclonal antibodies are produced by correct answers Hybridomas
The portion of an antigen that binds to an antibody or T cell receptor is called a(n) correct
answers Epitope
In immunofixation electrophoresis: correct answers The antigen is electrophoresed and then
monospecific antisera is reacted with it
Which of the following are L chains of antibody molecules? correct answers Kappa
Which immunoglobulin appears first in the primary immune response? correct answers IgM
Which antibody provides protection to the growing fetus because it is able to cross the placenta?
correct answers IgG
A substrate is first exposed to a patient's serum, then after washing, anti-human immunoglobulin
labeled with a fluorochrome is added. The procedure described is: correct answers indirect
fluorescence
In the enzyme- linked immunosorbent assay (ELISA) the visible reaction is due to a reaction
between correct answers Enzyme and substrate
Identical antibodies produced from a single clone of the plasma cells describes correct answers
Monoclonal antibodies
The detection of precipitation reactions depends on the presence of optimal proportions of
antigen and antibody. A patient's sample contains a large amount of antibody, but the reaction in
a test system containing antigen is negative. What has happened? correct answers Prozone
phenomenon
A Fab fragment consists of correct answers one L chain and one half of an H chain
Antibodies are produced by: correct answers B cells
Which of the following immunoglobin is the most efficient at agglutination: correct answers IgM
Which statement best describes flow-through immunoassays (immunochromatography-like a
pregnancy test from CVS)? correct answers They are designed for point of care testing
, Which test has the greatest sensitivity for antigen detection? correct answers ELISA
Which one of the following describes direct immunofluorescence array? correct answers
Conjugated reagent antibody reacts with antigen to form antigen-antibody complexes
Which immunoglobulin can cross the placenta? correct answers IgG
Which of the following immunoglobulins is present in the highest concentration in normal
human serum? correct answers IgG
A laboratory is evaluating an enzyme-linked immunosorbent assay (ELISA) for detecting an
antibody to cyclic citrullinated peptide (CCP), which is a marker for rheumatoid arthritis. The
laboratory includes serum from healthy volunteers and patients with other connective tissue
diseases in the evaluation. These specimens determine which factor of the assay? correct answers
Specificity
How does the secondary humoral immune response differ from the primary response? correct
answers The antibody levels produced are higher in the secondary immune response
What comprises the indicator system in an indirect ELISA for detecting antibody? correct
answers Enzyme-conjugated antibody + chromogenic substrate
The major class of immunoglobin found in adult human serum is correct answers IgG
After exposure to antigen, the first antibodies that can be detected belong to correct answers IgM
In hybridoma technology, the desirable fused cell is the: correct answers myeloma- lymphocyte
hybrid
Which class of immunoglobulin possesses delta heavy chains? correct answers IgD
Measurement of serum levels of which of the following immunoglobulins can serve as a
screening test for multiple allergies/parasitic infections? correct answers IgE
Blood is drawn from a patient for serological tests for a viral disease at the time of onset and
again 4 weeks later. The results of the test are considered diagnostic if the: correct answers
second antibody titer is at least 4x the first
Antibodies composed of IgG immunoglobin: correct answers can cross the placenta from mother
to fetus
The IgM molecule is a: correct answers pentamer
Immunoassays are based on the principle of: correct answers antibody recognition of a specific
antigen
