Microbiology LAB Exam #1 2025 Questions & Answers with 100% Correct Answers | Verified

Microscope- The Condenser - focuses the light ging into the objective lens Microscope- The Iris Diaphragm - adjusts the amount of light going into the objective lens Microscope- Nosepiece - holds the objective lenses and rotates to change from one objective to another Microscope- Coarse Objective Knob - moves the stage larger distances to help get the object in focus Microscope- Fine Adjustment Knob - moves the stage smaler increments to help get the object in better focus Microscope- Objective Lens - magnifies the object. It is located near the object. Microscope- Occular Lens - magnifies the image of the object from the objective lens. It is located near the eye. Steps in using the microscope - 1) scanner power in place 2) place slide of specimen stage in place with stage clips 3) Using the course adjustment knob, raise the stage all the way up and then down slowly until you can see image of specimen 4) Readjust with fine adjustment knob Describe the purpose of the oil immersion lens - Used when oil is added to the slide. Oil must touch the object and objective lens to that the light does not refract futher Parfocal - Parfocal microscopes are able to move from one objective to the next and still be in focus due to the length of the objective lenses