Microbiology Lab Exam 2025 Questions & Answers with 100% Correct Answers | Verified

Proper use of the microscope - all specimens must be viewed under cover glass, focus with scanning objectives first, use course adjustment knob, once in focus, use the fine focusing knob (when viewing with low or high power objectives) Depth of Field - refers to the thickness of the plane of focus Field of View - area that is visible when using the microscope Why use a microscope? - To view and study the microscopic world of organisms and objects less than 5mm in size that cohabitate with us on our globe. Ocular lens (location and job) - Area you place your eyes to see in scope (top of the tube) - magnifies the image formed by the objective lens Objective lenses (location and job) - the lenses right above where the sample is placed to be viewed. Can be changed to different magnifications (scanning, low, high). - magnifies the object Specimen holder (location and job) - located right under the objective lenses - holds specimen in place Mechanical Stage (location and job) - platform that you place your slides on - holds them in place with help of specimen holders and can be be moved up and down or left to right Light intensity control (location and job) - usually by or with the main power switch. Controls the intensity of the light x-y axis control knob (location and job) - attached to the stage (mechanical) - moves the stage along the x/y axis. One knob for back/front, one knob for left/right Course focusing knob (location and job) - located on side of microscope usually with fine focus knob - rough focus knob only used with high power objective