Chapter 3 - Microbiology Lab Exam 2025 Questions & Answers with 100% Correct Answers | Verified

Name the parts of the Compound Light Microscope: Eye piece, Objective, Stage, Light Control, Base, Focus - Dark-field Microscope - used when the microbes are not visible with the use of the light microscope; when microbes cannot be stained; when microbes would be distorted by staining Phase-Contrast Microscope - allows detailed visualization of the internal structures in living microbes Fluorescence Microscope - Immunofluorescence; detecting bacteria and other pathogens within cells and tissues; Dx of rabies and Syphilis Electron Microscope - ability to visualize viruses, the internal structure of cells, and other objects smaller than 0.2 um; uses a beam of electrons Transmission electron microscope/TEM - utilizes a focused beam of electrons that are emitted from an electron gun and pass thru a very thin section of the specimen Scanning electron microscope/SEM - section of specimen does NOT have to be thinly sliced like with the TEM; 3D views are obtained Which staining method is sufficient for determining the shape of bacteria & viewing basic structures? - simple stain