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Openstax Microbiology - Chapter 13 Exam Questions and Answers Latest Update 2025 Already Passed

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Openstax Microbiology - Chapter 13 Exam Questions and Answers Latest Update 2025 Already Passed Formites - Answers any non-living object that can be contaminated by microbes. The Centers for Disease Control and Prevention (CDC) and the National Institutes of Health (NIH) have established 4 classification levels called ________. - Answers Biological Safety Levels (BSL) BSL-1 - Answers Microorganisms not known to cause disease in healthy adults. BSL-2 organisms - Answers present a moderate environmental and or health hazard, can cause a disease but is treatable BSL-3 organisms - Answers have potential to cause lethal infections by inhalation. Divert from foreign location. Ex. HIV, West Nile Virus BSL-4 organisms - Answers Most dangerous and often fatal. These microbes are typically exotic, are easily transmitted by inhalation and cause infectious for which there's no treatment or vaccinations. Autoclave - Answers use moist heat, high pressure steam to serialize. Sterilization - Answers removal of ALL microbes. - lab uses autoclaves to sterilize tubes and plates. Asepsis - Answers treatments that decrease microbe numbers on LIVING TISSUES. Aseptic technique - Answers any procedure that minimizes contamination to a patient. - example: using sterile equipment and sterilizing or disposing of contaminated equipment properly. Antiseptic(s) - Answers chemicals that decrease microbe numbers. Commercial sterilization - Answers kills disease causing microbes, but not microbes that grow at high temperatures. does commercial sterilization truly sterilize? - Answers no, does not kill microbes that grow at high temperatures. What is commercial sterilization trying to eliminate and for what reason? - Answers intended to kill clostridium botulinum endospores to prevent botulism poisoning. Which requires longer exposure to formites, disinfection or sanitization? - Answers disinfection does disinfecting something achieve sterilization? - Answers no, does not destroy endospores. does sanitizing something achieve sterilization - Answers no, does not destroy endospores. sanitization define - Answers the process of reducting microbial contamination to an acceptable "safe" level. what two methods of reducing microbes is used to meet public health standards? - Answers disinfection and sanitization. _______ is an example of a natural disinfection. - Answers vinegar examples of antiseptics - Answers isopropyl alcohol; hydrogen peroxide degerming - Answers removes microbes by scrubbing, such as washing with ordinary soap. example: bathing/showering, using high pressure sprays chemicals that kill microbes. - Answers -cidal what do fungicidal chemicals do? - Answers kill fungi. chemicals that inhibit microbial growth. - Answers -static what do bacteriostatic chemicals do - Answers slow microbial growth sanitization - Answers removal of pathogens from objects to meet public health standards are food preservatives' -cidal or -static - Answers -static Bactericides (Disinfectants) - Answers Used to clean surfaces other than skin -kill bacteria Fungicides kill... - Answers Fungi bacteriostatic treatments - Answers inhibit growth of bacteria fungistatic - Answers An agent that inhibits the growth of fungi -static - Answers Thermal death point (TDP) - Answers the lowest temperature required to kill all microbes in a sample in 10 minutes Thermal death time (TDT) - Answers shortest length of time required to kill all test microbes at a specified temperature Antiseptics are: - Answers safe and effective to use on human skin

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Openstax Microbiology - Chapter 12 Modern Applications of Microbial Genetics Latest Update 2025
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biotechnology - Answers A form of technology that uses living organisms, usually genes, to modify
products, to make or modify plants and animals, or to develop other microorganisms for specific
purposes.

genetic engineering - Answers Process of making changes in the DNA code of living organisms

recombinant DNA technology - Answers technology that combines genes from different sources into a
single DNA molecule

recombinant DNA molecules - Answers molecules made by joining DNA from two different sources

transgenic - Answers term used to refer to an organism that contains genes from other organisms

molecular cloning - Answers isolation and incorporation of a piece of DNA into a vector so it can be
replicated and manipulated

restriction endonucleases - Answers an enzyme that cuts DNA at specific sites, producing small
fragments used in genetic engineering.

recognition site - Answers specific sequence of bases where a restriction enzyme cuts DNA

sticky ends - Answers Single stranded ends of DNA left after cutting with enzymes

blunt ends - Answers Restriction fragments with no overlapping ends and that never combine with
another type of DNA

anneal - Answers reduce brittleness and improve toughness by heating and cooling (metal or glass)

hybridization - Answers Breeding technique that involves crossing dissimilar individuals to bring together
the best traits of both organisms

ligation - Answers to tie off or bind

vectors - Answers quantities that have both a magnitude and a direction

polylinker site - Answers Engineered sequence in middle of Lac Z Gene

reporter gene - Answers A genetic marker included in recombinant DNA to indicate the presence of the
recombinant DNA in a host cell.

blue-white screening - Answers -Blue colonies do not have vector with foreign DNA inserted

-White colonies have foreign DNA inserted

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