Openstax Microbiology - Chapter 12 Modern Applications of Microbial Genetics Latest Update 2025
Fully Solved
biotechnology - Answers A form of technology that uses living organisms, usually genes, to modify
products, to make or modify plants and animals, or to develop other microorganisms for specific
purposes.
genetic engineering - Answers Process of making changes in the DNA code of living organisms
recombinant DNA technology - Answers technology that combines genes from different sources into a
single DNA molecule
recombinant DNA molecules - Answers molecules made by joining DNA from two different sources
transgenic - Answers term used to refer to an organism that contains genes from other organisms
molecular cloning - Answers isolation and incorporation of a piece of DNA into a vector so it can be
replicated and manipulated
restriction endonucleases - Answers an enzyme that cuts DNA at specific sites, producing small
fragments used in genetic engineering.
recognition site - Answers specific sequence of bases where a restriction enzyme cuts DNA
sticky ends - Answers Single stranded ends of DNA left after cutting with enzymes
blunt ends - Answers Restriction fragments with no overlapping ends and that never combine with
another type of DNA
anneal - Answers reduce brittleness and improve toughness by heating and cooling (metal or glass)
hybridization - Answers Breeding technique that involves crossing dissimilar individuals to bring together
the best traits of both organisms
ligation - Answers to tie off or bind
vectors - Answers quantities that have both a magnitude and a direction
polylinker site - Answers Engineered sequence in middle of Lac Z Gene
reporter gene - Answers A genetic marker included in recombinant DNA to indicate the presence of the
recombinant DNA in a host cell.
blue-white screening - Answers -Blue colonies do not have vector with foreign DNA inserted
-White colonies have foreign DNA inserted
Fully Solved
biotechnology - Answers A form of technology that uses living organisms, usually genes, to modify
products, to make or modify plants and animals, or to develop other microorganisms for specific
purposes.
genetic engineering - Answers Process of making changes in the DNA code of living organisms
recombinant DNA technology - Answers technology that combines genes from different sources into a
single DNA molecule
recombinant DNA molecules - Answers molecules made by joining DNA from two different sources
transgenic - Answers term used to refer to an organism that contains genes from other organisms
molecular cloning - Answers isolation and incorporation of a piece of DNA into a vector so it can be
replicated and manipulated
restriction endonucleases - Answers an enzyme that cuts DNA at specific sites, producing small
fragments used in genetic engineering.
recognition site - Answers specific sequence of bases where a restriction enzyme cuts DNA
sticky ends - Answers Single stranded ends of DNA left after cutting with enzymes
blunt ends - Answers Restriction fragments with no overlapping ends and that never combine with
another type of DNA
anneal - Answers reduce brittleness and improve toughness by heating and cooling (metal or glass)
hybridization - Answers Breeding technique that involves crossing dissimilar individuals to bring together
the best traits of both organisms
ligation - Answers to tie off or bind
vectors - Answers quantities that have both a magnitude and a direction
polylinker site - Answers Engineered sequence in middle of Lac Z Gene
reporter gene - Answers A genetic marker included in recombinant DNA to indicate the presence of the
recombinant DNA in a host cell.
blue-white screening - Answers -Blue colonies do not have vector with foreign DNA inserted
-White colonies have foreign DNA inserted
