Lab Report 3: Introduction to Spectrophotometry (Distinction)

Section 3: Introduction to Spectrophotometry


Statement of the Problem:
The experiment's objectives are to determine the bromophenol blue dye's wavelength of
maximum absorbance (Amax) while investigating the relationship between dye concentration
and absorbance. This involves identifying the Amax, the point at which the spectrophotometer
is most responsive to variations in absorbance, and analysing the connection between
absorbance and different bromophenol blue concentrations made from a stock solution.



Hypothesis:
It is possible to determine the bromophenol blue dye's wavelength of maximum absorbance
(Amax), and the absorbance at this wavelength will increase in the exact proportion to the dye's
concentration until a saturation point is reached.


Materials:
 Distilled Water
 Pipette
 Cuvette
 Cuvette holder
 Spectrophotometer
 bromophenol blue solution