Section1: Introduction to the Laboratory Environment (Using
pipettes)
Statement of the Problem:
• The aim of this laboratory report is to use a serological pipette and a micropipette to
transfer and measure an accurate volume of a solution.
Hypothesis:
At constant pressure and temperature, distilled water has a constant density.
Once the water has been distributed, its volume can be determined by weighing
it. Pipettes are calibrated using the gravimetric method. The ambient
temperature, air pressure, and relative humidity all affect how accurately
observations are made while determining the volume of water. The computed
water volume is then compared to the theoretical amount to evaluate the
pipette's accuracy and precision.
Using pipettes is considered a crucial aspect in laboratory work to ensure
experimental accuracy by allowing approximate measurement and transfer of
liquid volumes
Materials:
Serological pipette (and pipette pump)
Micropipette (P200, P1000)
Conical flask
Electronic flask
Beaker
Pasteur pipette
Procedure:
The procedure requires multiple steps including using different instruments, I have
split this in 2 parts:
Part 1: Using a micropipette
I started by resetting the weighing balance to 0 to ensure accuracy. I chose a pipette
that could transfer 1000 µL, adjusted the volume, and attached the proper tip. To
pull in liquid without air bubbles, I held the pipette vertically, pulled the plunger to
the first stop, submerged the tip in water, and then slowly released the plunger.
ejecting the tip into a Disposafe jar, I made sure the water was fully delivered by
pressing to the first stop on the weighing boat wall and then to the second stop
pipettes)
Statement of the Problem:
• The aim of this laboratory report is to use a serological pipette and a micropipette to
transfer and measure an accurate volume of a solution.
Hypothesis:
At constant pressure and temperature, distilled water has a constant density.
Once the water has been distributed, its volume can be determined by weighing
it. Pipettes are calibrated using the gravimetric method. The ambient
temperature, air pressure, and relative humidity all affect how accurately
observations are made while determining the volume of water. The computed
water volume is then compared to the theoretical amount to evaluate the
pipette's accuracy and precision.
Using pipettes is considered a crucial aspect in laboratory work to ensure
experimental accuracy by allowing approximate measurement and transfer of
liquid volumes
Materials:
Serological pipette (and pipette pump)
Micropipette (P200, P1000)
Conical flask
Electronic flask
Beaker
Pasteur pipette
Procedure:
The procedure requires multiple steps including using different instruments, I have
split this in 2 parts:
Part 1: Using a micropipette
I started by resetting the weighing balance to 0 to ensure accuracy. I chose a pipette
that could transfer 1000 µL, adjusted the volume, and attached the proper tip. To
pull in liquid without air bubbles, I held the pipette vertically, pulled the plunger to
the first stop, submerged the tip in water, and then slowly released the plunger.
ejecting the tip into a Disposafe jar, I made sure the water was fully delivered by
pressing to the first stop on the weighing boat wall and then to the second stop
