Soorten microscopen
Resolution eye = 0,1mm
Microscope Resolution
Limit
Compound Bright Field Microscope 1-10nm
Epifluorescence microscope 200nm
With Ab 1-10nm
Multiphoton microscopy (3photons) 800µm
Near field scanning optical 20nm
microscope
Indirect way (TIRF) 100nm
Far field scanning optical microscope 100nm
(SIM)
Transmission Electron Microscopy 0,1nm
(TEM)
Scanning Electron Microscopy (SEM) 1-10nm
FIB-SEM 1nm
, Compound microscopy
Algemeen:
Bright field microscope
Resolution limit: 1-10nm
Soort
o Upright microscope
Objective above the sample
o Inverted microscope
Objective below the sample (look at the cells from
below)
Mostly 2 lenses: objective and ocular
‘Anatomy’
o Light – condenser – sample – objective – ocular – eye
Conjugate planes
o Lamp filament
o Field diafragm
o Between objective and ocular
o Lens of the eye
Dark field microscopy
Algemeen:
Dark field
o No sample: Light in very inclined angle (contender annulus)
through the sample light not be captured by objective
o Sample: Only capture scattered light
Zernike phase contrast:
o Condenser annulus causes light to fall outside of the
objective
o Phase plate shifts the phase os unrefracted light allow
interference
Resolution eye = 0,1mm
Microscope Resolution
Limit
Compound Bright Field Microscope 1-10nm
Epifluorescence microscope 200nm
With Ab 1-10nm
Multiphoton microscopy (3photons) 800µm
Near field scanning optical 20nm
microscope
Indirect way (TIRF) 100nm
Far field scanning optical microscope 100nm
(SIM)
Transmission Electron Microscopy 0,1nm
(TEM)
Scanning Electron Microscopy (SEM) 1-10nm
FIB-SEM 1nm
, Compound microscopy
Algemeen:
Bright field microscope
Resolution limit: 1-10nm
Soort
o Upright microscope
Objective above the sample
o Inverted microscope
Objective below the sample (look at the cells from
below)
Mostly 2 lenses: objective and ocular
‘Anatomy’
o Light – condenser – sample – objective – ocular – eye
Conjugate planes
o Lamp filament
o Field diafragm
o Between objective and ocular
o Lens of the eye
Dark field microscopy
Algemeen:
Dark field
o No sample: Light in very inclined angle (contender annulus)
through the sample light not be captured by objective
o Sample: Only capture scattered light
Zernike phase contrast:
o Condenser annulus causes light to fall outside of the
objective
o Phase plate shifts the phase os unrefracted light allow
interference
