Summary Topic 6 DNA profiling

DNA Profiling
23 000 =
90000 proteins
genes
.




Exons lutons
genes
are made
of and
S.I units
Exons
coding
=




Introns
coding
=
non




Es 12 En 12 Es Is

↳
different combinations of Exons


different codon
-




sequence
leng he
of mRNA
-
-




= different amino
Sequence dcid


different polypeptide laught
Spliceosome cuts out introns
through post
transcriptual splice up
between Exors
reforms phosphodiester bonds
↳


Post translation the
purpose of
the
modification is

golgi .




Introns identifiable characteristics molecular
for
-




phylogeny and DNA
profiling

STR's >
- Short Tandem repeats (2-50 bases)

base repeat
e .



.
g agtaagtaagta-4
alleles have
different number
of repeats
↳

, Maternal and Paternal chromosomes
may af
of repeats
different
have number STR
na same
gene 102i (homologous pairs)




E #
S.I units




7 repeats a
repeats


Principles and rationale of DNA profiling
.
1 DNA
Everyones is
unique

.
2 Except (identical)
twins
in
monozygotic
3 . Based on short tandem
repeats in Introns
.


4.
Compare bands of DNA (DNA profile) gel electrophesis
5 10 loci compared to be reliable
gene
.




Forensics
-


find tissue
- hair
,
blood

DNA break
from tissue membranes
->
>
down and
-




histore proteins
. DNA
1
may
be
partially
Contamination
decomposed
DNA
of
.
2