Genetics Chapter 21 (Molecular
Technologies) UPDATED ACTUAL
Questions and CORRECT Answers
TSeps for selection for bacterial cells with a recombinant factor - CORRECT ANSWER -
1. Bacterial cells are transformed
2. Vector must contain selectable marker
3. Vector must contain a second selectable marker
Nircroarray - CORRECT ANSWER -
Polymerase Chain reaction (PCR) - CORRECT ANSWER - A method for gene cloning
How is PCR used? - CORRECT ANSWER - To amplify a segment of DNA
Gene cloning - CORRECT ANSWER - A DNA sequence of interest is located and copied
What does PCR require? - CORRECT ANSWER - 1. Template DNA(DNA to be
amplified)
2. Primers
3. DNA nucleotides (A, G, T, C)
4. Taq polymerase (heat stable)
What are the primers? - CORRECT ANSWER - Short DNA sequences (oligonucleotides)
complimentary to the target DNA
What are the steps of PCR? - CORRECT ANSWER - 1. Denaturation of DNA sample
2. Primer annealing
, 3. Primer extension
What are the uses of PCR? - CORRECT ANSWER - 1. DNA fingerprinting
2. Amplification of DNA for sequencing
3. Detecting the presence of a DNA sequence
4. Isolating a gene for insertion into a vector
Why is the detection of the presence of a DNA sequence important? - CORRECT
ANSWER - To detect infection or a genetic disorder
If cDNA is desired, what needs to happen? - CORRECT ANSWER - Reverse transcriptase
can make cDNA from mRNA
What is cDNA? - CORRECT ANSWER - (Complimentary DNA) DNA copy of mRNA
cDNA does not contain what? - CORRECT ANSWER - Introns
The steps to make cDna - CORRECT ANSWER - 1. Isolate the mRNA
2. Add a primer that binds to the polyA tail
3. Add reverse transcriptase
4. Do the PCR now that you have the cDNA
What does reverse transcriptase do? - CORRECT ANSWER - Synthesizes DNA from
mRNA
What are the uses of cDNA? - CORRECT ANSWER - May need to isolate the coding
sequence of a gene or determine whether a particular gene is expressed
Technologies) UPDATED ACTUAL
Questions and CORRECT Answers
TSeps for selection for bacterial cells with a recombinant factor - CORRECT ANSWER -
1. Bacterial cells are transformed
2. Vector must contain selectable marker
3. Vector must contain a second selectable marker
Nircroarray - CORRECT ANSWER -
Polymerase Chain reaction (PCR) - CORRECT ANSWER - A method for gene cloning
How is PCR used? - CORRECT ANSWER - To amplify a segment of DNA
Gene cloning - CORRECT ANSWER - A DNA sequence of interest is located and copied
What does PCR require? - CORRECT ANSWER - 1. Template DNA(DNA to be
amplified)
2. Primers
3. DNA nucleotides (A, G, T, C)
4. Taq polymerase (heat stable)
What are the primers? - CORRECT ANSWER - Short DNA sequences (oligonucleotides)
complimentary to the target DNA
What are the steps of PCR? - CORRECT ANSWER - 1. Denaturation of DNA sample
2. Primer annealing
, 3. Primer extension
What are the uses of PCR? - CORRECT ANSWER - 1. DNA fingerprinting
2. Amplification of DNA for sequencing
3. Detecting the presence of a DNA sequence
4. Isolating a gene for insertion into a vector
Why is the detection of the presence of a DNA sequence important? - CORRECT
ANSWER - To detect infection or a genetic disorder
If cDNA is desired, what needs to happen? - CORRECT ANSWER - Reverse transcriptase
can make cDNA from mRNA
What is cDNA? - CORRECT ANSWER - (Complimentary DNA) DNA copy of mRNA
cDNA does not contain what? - CORRECT ANSWER - Introns
The steps to make cDna - CORRECT ANSWER - 1. Isolate the mRNA
2. Add a primer that binds to the polyA tail
3. Add reverse transcriptase
4. Do the PCR now that you have the cDNA
What does reverse transcriptase do? - CORRECT ANSWER - Synthesizes DNA from
mRNA
What are the uses of cDNA? - CORRECT ANSWER - May need to isolate the coding
sequence of a gene or determine whether a particular gene is expressed
