Molecular Biology Exam #2 UPDATED
ACTUAL Questions and CORRECT
Answers
Which of the following is a natural substrate ( or component) used for DNA replication by DNA
polymerase III? - CORRECT ANSWER - RNA short fragments
Which of the following is not a component found in a replication fork? - CORRECT
ANSWER - Protease
The majority of the new nucleotides is added by ___ during E. Coli DNA replication -
CORRECT ANSWER - DNA polymerase III
Which of the following ion depletion will directly affect DNA polymerase III function? -
CORRECT ANSWER - Mg2+ and Zinc3+
Which of the following is false regarding the function of the metal ions in the DNA polymerase
catalytic pocket is to: - CORRECT ANSWER - Stabilizes the template DNA
Which of the following amino acid residue(s) of DNA polymerase doesn't play a critical role in
the catalytic site of the DNA polymerase during DNA replication? - CORRECT
ANSWER - Histidine
DNA polymerase enzyme cannot catalyze DNA synthesis:
a. In the absence of a 3'OH
b. In the absence of primer-template junction
c. In a 3' to 5' direction
d. All of the above - CORRECT ANSWER - All of the above
, For DNA replication in eukaryotic cells, which of the following is not involved? - CORRECT
ANSWER - DNA polymerase III
Which of the following is not a DNA fragment? - CORRECT ANSWER - Initiation
Which of the following is not directly related with the initiation of replication? - CORRECT
ANSWER - Histone Methlyation
Which of the following is unrelated to telomere synthesis or stability? - CORRECT
ANSWER - Primer synthesis
Which of the following is not a component of a replicator? - CORRECT ANSWER -
Polymerase III recruitinng site
Which of the following is not true:
a. Seq A binds to semimethylated GATC
b. Seq A prevents DAM mediated DAN methylation
c. Seq A usually binds semimethylated GATC for a short period of time
d. Seq A is an important regulator for DNA replication fidelity control during replication -
CORRECT ANSWER - Seq A is an important regulator for DNA replication fidelity
control during replication
To initiate DNA replication in E. coli, which of the following characteristics of protein DnaA is
not true - CORRECT ANSWER - Binding semimethylated DNA only
Which of the following is true
a. "Priming protein" is a primase
b. " priming protein" is an RNA polymerase
c. "Priming protein" exists in all eukaryotes
ACTUAL Questions and CORRECT
Answers
Which of the following is a natural substrate ( or component) used for DNA replication by DNA
polymerase III? - CORRECT ANSWER - RNA short fragments
Which of the following is not a component found in a replication fork? - CORRECT
ANSWER - Protease
The majority of the new nucleotides is added by ___ during E. Coli DNA replication -
CORRECT ANSWER - DNA polymerase III
Which of the following ion depletion will directly affect DNA polymerase III function? -
CORRECT ANSWER - Mg2+ and Zinc3+
Which of the following is false regarding the function of the metal ions in the DNA polymerase
catalytic pocket is to: - CORRECT ANSWER - Stabilizes the template DNA
Which of the following amino acid residue(s) of DNA polymerase doesn't play a critical role in
the catalytic site of the DNA polymerase during DNA replication? - CORRECT
ANSWER - Histidine
DNA polymerase enzyme cannot catalyze DNA synthesis:
a. In the absence of a 3'OH
b. In the absence of primer-template junction
c. In a 3' to 5' direction
d. All of the above - CORRECT ANSWER - All of the above
, For DNA replication in eukaryotic cells, which of the following is not involved? - CORRECT
ANSWER - DNA polymerase III
Which of the following is not a DNA fragment? - CORRECT ANSWER - Initiation
Which of the following is not directly related with the initiation of replication? - CORRECT
ANSWER - Histone Methlyation
Which of the following is unrelated to telomere synthesis or stability? - CORRECT
ANSWER - Primer synthesis
Which of the following is not a component of a replicator? - CORRECT ANSWER -
Polymerase III recruitinng site
Which of the following is not true:
a. Seq A binds to semimethylated GATC
b. Seq A prevents DAM mediated DAN methylation
c. Seq A usually binds semimethylated GATC for a short period of time
d. Seq A is an important regulator for DNA replication fidelity control during replication -
CORRECT ANSWER - Seq A is an important regulator for DNA replication fidelity
control during replication
To initiate DNA replication in E. coli, which of the following characteristics of protein DnaA is
not true - CORRECT ANSWER - Binding semimethylated DNA only
Which of the following is true
a. "Priming protein" is a primase
b. " priming protein" is an RNA polymerase
c. "Priming protein" exists in all eukaryotes
