Transcription
PCR steps
denaturation: Using heat to break apart the DNA strand into single strands
Annealing: allowed primers to attach to the single strand of DNA during the
cool-down period
Extension: DNA polymerase added on the DNA sequence
Restriction Enzymes
used to cut DNA in specific locations
Originally isolated from bacteria and other microorganisms
These enzymes cleave restriction sites (which are often 4 – 6 bp long in a
palindromic manner)
Central Dogma
DNA → RNA = transcription
RNA → Protein - Translation
Genotype: what's in your DNA
Phenotype: how genotypes are expressed physically
Transcription
DNA → gets broken apart
One is a coding strand, that is not used to synthesize RNA
Template strand: the strand RNA is transcribed off of
Difference between DNA and RNA
DNA is double-stranded while RNA is single-stranded
DNA is storage & in the nucleus
Transcription 1
PCR steps
denaturation: Using heat to break apart the DNA strand into single strands
Annealing: allowed primers to attach to the single strand of DNA during the
cool-down period
Extension: DNA polymerase added on the DNA sequence
Restriction Enzymes
used to cut DNA in specific locations
Originally isolated from bacteria and other microorganisms
These enzymes cleave restriction sites (which are often 4 – 6 bp long in a
palindromic manner)
Central Dogma
DNA → RNA = transcription
RNA → Protein - Translation
Genotype: what's in your DNA
Phenotype: how genotypes are expressed physically
Transcription
DNA → gets broken apart
One is a coding strand, that is not used to synthesize RNA
Template strand: the strand RNA is transcribed off of
Difference between DNA and RNA
DNA is double-stranded while RNA is single-stranded
DNA is storage & in the nucleus
Transcription 1
