ASCP CG Certification Practice 2025 UPDATE LATEST EXAM VERSION MOST TESTED QUESTIONS WITH
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
What is the best agent to use against mycoplasma? - correct answer BM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - correct
answer comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - correct answer Proteolytic enzymes - break down
tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - correct answer Colcemid - mitotic spindle poison that halts cells at
metaphase
Samples that arrive at the cytogenetics lab should first be: - correct answer Logged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - correct answer T-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is
the MOST important to document? - correct answer Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - correct
answer Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - correct answer
Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around
the mets, what is the most likely cause? - correct answer innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of cultures? - correct
answer Asceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - correct answer prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse,
what could help fix the issue? - correct answer Increading the drying time by using warm slides
What is true of an inverted microscope? - correct answer the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - correct answer DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
,ASCP CG Certification Practice 2025 UPDATE LATEST EXAM VERSION MOST TESTED QUESTIONS WITH
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - correct answer Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
correct answer Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - correct answer
Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - correct answer Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast
scope is: - correct answer Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - correct answer 45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? - correct answer Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? - correct
answer The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up amniotic
fluid samples, the specimen could become contaminated with: - correct answer Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain
used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination
, ASCP CG Certification Practice 2025 UPDATE LATEST EXAM VERSION MOST TESTED QUESTIONS WITH
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
What about the metaphases on a slide are affected by slide making? - correct answer Cell
density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome? - correct
answer High resolution banding and FISH for the Prader-Willi locus on 15q11.2
When capturing a metaphase, a tech cannot get the met into focus, what is the most likely
cause? - correct answer The camera is not parfocal (in focus with the microscpe objectives)
with the microscope
Unstained chromosome preparation should be viewed using which scope? - correct answer
Phase contrast condenser
The minimum band level necessary to detect prader-willi syndrome is? - correct answer 550 at
the lowest
15q11.2 is visible at that level
See CAP regulation CYG.42300
A regulatory inspecter requests a copy of a lab patient's record for his inspection files, the tech
should: - correct answer Deny the inspector a copy of the record
See HIPAA compliance policy
According to CAP regulations, what is the minimum band level for consitutional specimens? -
correct answer 400
The suggested level for most constitutional specimen is 550, but 400 is the lowest acceptible
level
See CAP regulation CYG.42200
Placental tissue is submitted for chromosome analysis, to optimize the liklihood of obtaining
fetal cells, where should the tech select tissue from? - correct answer The fetal side of the
spcimen (when possible to be identified from the decidua)
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
What is the best agent to use against mycoplasma? - correct answer BM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: - correct
answer comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - correct answer Proteolytic enzymes - break down
tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - correct answer Colcemid - mitotic spindle poison that halts cells at
metaphase
Samples that arrive at the cytogenetics lab should first be: - correct answer Logged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? - correct answer T-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is
the MOST important to document? - correct answer Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - correct
answer Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? - correct answer
Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around
the mets, what is the most likely cause? - correct answer innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of cultures? - correct
answer Asceptic technique
When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - correct answer prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out and sparse,
what could help fix the issue? - correct answer Increading the drying time by using warm slides
What is true of an inverted microscope? - correct answer the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - correct answer DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
,ASCP CG Certification Practice 2025 UPDATE LATEST EXAM VERSION MOST TESTED QUESTIONS WITH
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - correct answer Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? -
correct answer Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - correct answer
Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - correct answer Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase contrast
scope is: - correct answer Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - correct answer 45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? - correct answer Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? - correct
answer The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up amniotic
fluid samples, the specimen could become contaminated with: - correct answer Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain
used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination
, ASCP CG Certification Practice 2025 UPDATE LATEST EXAM VERSION MOST TESTED QUESTIONS WITH
VERIFIED ANSWERS GRADED A+ ASSURED SUCCESS
What about the metaphases on a slide are affected by slide making? - correct answer Cell
density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome? - correct
answer High resolution banding and FISH for the Prader-Willi locus on 15q11.2
When capturing a metaphase, a tech cannot get the met into focus, what is the most likely
cause? - correct answer The camera is not parfocal (in focus with the microscpe objectives)
with the microscope
Unstained chromosome preparation should be viewed using which scope? - correct answer
Phase contrast condenser
The minimum band level necessary to detect prader-willi syndrome is? - correct answer 550 at
the lowest
15q11.2 is visible at that level
See CAP regulation CYG.42300
A regulatory inspecter requests a copy of a lab patient's record for his inspection files, the tech
should: - correct answer Deny the inspector a copy of the record
See HIPAA compliance policy
According to CAP regulations, what is the minimum band level for consitutional specimens? -
correct answer 400
The suggested level for most constitutional specimen is 550, but 400 is the lowest acceptible
level
See CAP regulation CYG.42200
Placental tissue is submitted for chromosome analysis, to optimize the liklihood of obtaining
fetal cells, where should the tech select tissue from? - correct answer The fetal side of the
spcimen (when possible to be identified from the decidua)
