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Microbio 150 Lab Exam 1 PVCC Questions
And Answers 100% Pass.
When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective - Answer✔Always
Where should used gloves be disposed of in the microbiology lab - Answer✔In the bio-hazard
waste container
Before putting a dust cover over the microscope and placing it in the cabinet, which objective
lens should be rotated over the stage - Answer✔4x
On the microscope, what concentrates the light on the specimen? - Answer✔Condenser lens
On the microscope, what bends light passing through the specimen to create a real image -
Answer✔Objective lens
On the microscope, what allows for more or less light to be directed onto the specimen -
Answer✔Iris diaphragm
On the microscope, what gives you clarity of the image - Answer✔Resolution
Why is desiccating your inoculating loop important? When should this be done? - Answer✔To
ensure it is sterilized, done so no cross contamination happens
During an aseptic transfer, when do you pass the mouth of the test tube through the flame? -
Answer✔Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain a pure culture from a
__________. - Answer✔Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to - Answer✔Attach it firmly
to the slide
The term that refers to the purposeful addition of microorganisms into a nutrient medium is -
Answer✔Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop around while waiting
for it to dry. - Answer✔False
Microbio 150 Lab Exam 1 PVCC Questions
And Answers 100% Pass.
When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective - Answer✔Always
Where should used gloves be disposed of in the microbiology lab - Answer✔In the bio-hazard
waste container
Before putting a dust cover over the microscope and placing it in the cabinet, which objective
lens should be rotated over the stage - Answer✔4x
On the microscope, what concentrates the light on the specimen? - Answer✔Condenser lens
On the microscope, what bends light passing through the specimen to create a real image -
Answer✔Objective lens
On the microscope, what allows for more or less light to be directed onto the specimen -
Answer✔Iris diaphragm
On the microscope, what gives you clarity of the image - Answer✔Resolution
Why is desiccating your inoculating loop important? When should this be done? - Answer✔To
ensure it is sterilized, done so no cross contamination happens
During an aseptic transfer, when do you pass the mouth of the test tube through the flame? -
Answer✔Before and after dipping loop in
During the broth to slide aseptic transfer, a __________ was used to obtain a pure culture from a
__________. - Answer✔Loop, broth
Prior to staining, smears of microorganisms are heat-fixed in order to - Answer✔Attach it firmly
to the slide
The term that refers to the purposeful addition of microorganisms into a nutrient medium is -
Answer✔Inoculation
True or false: In the microbiology lab, it is acceptable to move your loop around while waiting
for it to dry. - Answer✔False
