MicroBio Lab Exam 1 UPDATED ACTUAL
Questions and CORRECT Answers
Which designates an organism that requires the highest degree of training and safety
precautions?c - CORRECT ANSWER - BSL-4
T/F A molecule's shape affects its function (how it works) - CORRECT ANSWER - True
Broken glass that MAY be contaminated with microorganisms goes into a: - CORRECT
ANSWER - biohazard sharps container
Which of these is NOT a proper part of dealing with spills or breakage? - CORRECT
ANSWER - look up the proper procedure on your cell phone
Which of these is the LEAST likely to cause a safety problem? - CORRECT ANSWER -
BSL-1
Which amino acids like to interact with nonpolar amino acids? - CORRECT ANSWER -
nonpolar
Why do you use a Bunsen burner in the microbiology lab? - CORRECT ANSWER - to
sterilize tools
A controlled experiment is one in which: - CORRECT ANSWER - all variables except one
are kept constant
Which of these are the three forces that control molecular interaction? - CORRECT
ANSWER - -hydrophobicity
-charge
-shape
,Which of the following is NOT included in the standard PPE required during our lab? -
CORRECT ANSWER - respirators
An enzyme interacts with its substrate based on: - CORRECT ANSWER - -shape
-hydrophobicity
-charge
T/F Correlation means 2 things changing together, or is an assessment of whether one variable is
associated with a change in another. - CORRECT ANSWER - True
To prepare a broth or agar, the material is ________ and must be ___________________. -
CORRECT ANSWER - dry, dissolved in hot water
To take care of tubes that you have used in lab, you should: - CORRECT ANSWER - take
the tape off the tubes and put on wire shelves to be autoclaved and cleaned
Humans contain _____________ human cells, and ________________ microorganism cells. -
CORRECT ANSWER - 10 trillion, 100 trillion
It is important to keep samples from the throat and tongue separate, because: - CORRECT
ANSWER - the normal flora of the tongue is different from the throat
What is the point of doing a "streak for isolation" 4 quadrant streak plate? - CORRECT
ANSWER - isolated colonies
In the Ubiquity experiment (read only this semester), the set up is as follows:
Streak a plate from the lab bench, streak a plate from the lab floor, and leave one plate closed
without touching it. The closed plate serves in order to: - CORRECT ANSWER - -be an
uninoculated control
, -show whether the agar was contaminated while making it
The key to proper inoculations is to sterilize the tool: - CORRECT ANSWER - by flaming
it between each step
Gram Staining is dependent upon: - CORRECT ANSWER - the structure of the bacterial
cell wall
T/F Anthrax will test positive in the endospore stain - CORRECT ANSWER - True
What color are Gram negative cells at the end of the stain? - CORRECT ANSWER - Pink
It is called a "Negative Stain" because the stain has a negative charge, and is therefore: -
CORRECT ANSWER - repelled by the bacterial surface
Algae are: - CORRECT ANSWER - eukaryotes
able to do photosynthesis
Why do we not use a heat-fixed emulsion on capsule stains? - CORRECT ANSWER -
Heat would melt the capsules
Giardia is often acquired from: - CORRECT ANSWER - clear flowing water
pets
What color would human cells be at the end of the Gram stain? - CORRECT ANSWER -
Pink
The term "elevation" means: - CORRECT ANSWER - a description of the height of a
colony and the shape of its "mountain top"
Questions and CORRECT Answers
Which designates an organism that requires the highest degree of training and safety
precautions?c - CORRECT ANSWER - BSL-4
T/F A molecule's shape affects its function (how it works) - CORRECT ANSWER - True
Broken glass that MAY be contaminated with microorganisms goes into a: - CORRECT
ANSWER - biohazard sharps container
Which of these is NOT a proper part of dealing with spills or breakage? - CORRECT
ANSWER - look up the proper procedure on your cell phone
Which of these is the LEAST likely to cause a safety problem? - CORRECT ANSWER -
BSL-1
Which amino acids like to interact with nonpolar amino acids? - CORRECT ANSWER -
nonpolar
Why do you use a Bunsen burner in the microbiology lab? - CORRECT ANSWER - to
sterilize tools
A controlled experiment is one in which: - CORRECT ANSWER - all variables except one
are kept constant
Which of these are the three forces that control molecular interaction? - CORRECT
ANSWER - -hydrophobicity
-charge
-shape
,Which of the following is NOT included in the standard PPE required during our lab? -
CORRECT ANSWER - respirators
An enzyme interacts with its substrate based on: - CORRECT ANSWER - -shape
-hydrophobicity
-charge
T/F Correlation means 2 things changing together, or is an assessment of whether one variable is
associated with a change in another. - CORRECT ANSWER - True
To prepare a broth or agar, the material is ________ and must be ___________________. -
CORRECT ANSWER - dry, dissolved in hot water
To take care of tubes that you have used in lab, you should: - CORRECT ANSWER - take
the tape off the tubes and put on wire shelves to be autoclaved and cleaned
Humans contain _____________ human cells, and ________________ microorganism cells. -
CORRECT ANSWER - 10 trillion, 100 trillion
It is important to keep samples from the throat and tongue separate, because: - CORRECT
ANSWER - the normal flora of the tongue is different from the throat
What is the point of doing a "streak for isolation" 4 quadrant streak plate? - CORRECT
ANSWER - isolated colonies
In the Ubiquity experiment (read only this semester), the set up is as follows:
Streak a plate from the lab bench, streak a plate from the lab floor, and leave one plate closed
without touching it. The closed plate serves in order to: - CORRECT ANSWER - -be an
uninoculated control
, -show whether the agar was contaminated while making it
The key to proper inoculations is to sterilize the tool: - CORRECT ANSWER - by flaming
it between each step
Gram Staining is dependent upon: - CORRECT ANSWER - the structure of the bacterial
cell wall
T/F Anthrax will test positive in the endospore stain - CORRECT ANSWER - True
What color are Gram negative cells at the end of the stain? - CORRECT ANSWER - Pink
It is called a "Negative Stain" because the stain has a negative charge, and is therefore: -
CORRECT ANSWER - repelled by the bacterial surface
Algae are: - CORRECT ANSWER - eukaryotes
able to do photosynthesis
Why do we not use a heat-fixed emulsion on capsule stains? - CORRECT ANSWER -
Heat would melt the capsules
Giardia is often acquired from: - CORRECT ANSWER - clear flowing water
pets
What color would human cells be at the end of the Gram stain? - CORRECT ANSWER -
Pink
The term "elevation" means: - CORRECT ANSWER - a description of the height of a
colony and the shape of its "mountain top"
