Microbiology Lab Exam

MICROBIOLOGY LAB EXAM #1


Microscope-aoTheaoCondenserao-aoans--
focusesaotheaolightaogingaointoaotheaoobjectiveaolens

Microscope-aoTheaoIrisaoDiaphragmao-aoans--
adjustsaotheaoamountaoofaolightaogoingaointoaotheaoobjectiveaolens

Microscope-aoNosepieceao-aoans--
holdsaotheaoobjectiveaolensesaoandaorotatesaotoaochangeaofromaooneaoobjectiveaotoaoanot
her

Microscope-aoCoarseaoObjectiveaoKnobao-aoans--
movesaotheaostageaolargeraodistancesaotoaohelpaogetaotheaoobjectaoinaofocus

Microscope-aoFineaoAdjustmentaoKnobao-aoans--
movesaotheaostageaosmaleraoincrementsaotoaohelpaogetaotheaoobjectaoinaobetteraofocus

Microscope-aoObjectiveaoLensao-aoans--
magnifiesaotheaoobject.aoItaoisaolocatedaonearaotheaoobject.

Microscope-aoOccularaoLensao-aoans--
magnifiesaotheaoimageaoofaotheaoobjectaofromaotheaoobjectiveaolens.aoItaoisaolocatedaonear
aotheaoeye.


Stepsaoinaousingaotheaomicroscopeao-aoans--1)aoscanneraopoweraoinaoplace
2)aoplaceaoslideaoofaospecimenaostageaoinaoplaceaowithaostageaoclips
3)aoUsingaotheaocourseaoadjustmentaoknob,aoraiseaotheaostageaoallaotheaowayaoupaoandaot
henaodownaoslowlyaountilaoyouaocanaoseeaoimageaoofaospecimen
4)aoReadjustaowithaofineaoadjustmentaoknob

Describeaotheaopurposeaoofaotheaooilaoimmersionaolensao-aoans--
Usedaowhenaooilaoisaoaddedaotoaotheaoslide.aoOilaomustaotouchaotheaoobjectaoandaoobjecti
veaolensaotoaothataotheaolightaodoesaonotaorefractaofuther

Parfocalao-aoans--
Parfocalaomicroscopesaoareaoableaotoaomoveaofromaooneaoobjectiveaotoaotheaonextaoandao
stillaobeaoinaofocusaodueaotoaotheaolengthaoofaotheaoobjectiveaolenses

, Resolutionao-aoans--
Theaoabilityaotoaodistinguishaobetweenaotwoaoobjectsaothataoareaoveryaocloseaotogether.

Whataoisaoresolvingaopower?ao-aoans--Theaomeasurementaoofaoresolution

Whataoareaotwoaowaysaotoaoincreaseaoresolvingaopower?ao-aoans--
1.aoByaodecreasingaotheaowavelengthaoofaolightao(usingaoaaoblueaofilter)ao2.aoIncreasingaot
heaonumericalaoaperature

Whataounitsaoareaousedaotoaoexpressaoresolvingaopower?ao-aoans--nmaooraonanometers

Whataoisaobetter,aosmalleraooraolargeraoresolvingaopower?ao-aoans--Smalleraoisaobetter
Lightaomicroscopesaohaveaoaao200nmaoresolvingaopower
Electronaomicroscopesaohaveaoaao.2nmaoresolvingaopower

Whataoisaochromaticaoaberration?ao-aoans--
Theaoeffectaowhenaomanyaocolorsaoareaoseenaotowardaotheaoedgeaoofaotheaomicroscopeao
fieldaoofaovisionao(likeaoaaoprisim)

Howaoareaomicroscopesaocorrectedaoforaochromaticaoaberration?ao-aoans--
Withaoaaospecialaoachromaticaolensao(correctedaoforaoredaoandaoblueaolight)aooraobyaousin
gaoaaoblueaofilter.aoTheaolabaohasaoblueaofiltersaoinaotheiraomicroscopesaowhichaoalsoaoincr
easeaoresolution.

Describeaotheaoprocessaoofaomakingaoaaowet-mountaoslideao-aoans--
1)aoAddaotheaospecimenaotoaotheaoslide
2)aoTouchaotheaoedgeaoofaotheaocoveraoslipaowithaooneaoedgeaoofaodropaoofaospecimen.
3)aoLetaotheaocoveraoslipaodropaograduallyaotoaotheaoslide,aocoveringaotheaodropaoofaospeci
men.

Describeaotheaopurposeaoofaomakingaoaaowet-mountaoslideao-aoans--
Toaobeaoableaotoaoseeaolivingaoorganismsaounderaotheaomicroscopeaoinaotheiraonaturalaost
ate.

Whataoisaotheaoadvantageaoofaousingaoaaowet-mountaoslide?ao-aoans--
Canaoseeaomovementaoandaodetermineaoifaomicrobesaopossesaotheiraoownaomethodaoofao
motility.aoCanaoalsoaoseeaotheaoactualaoshapeaoandaoarrangementaoofaomicrobes.

Whataoisaotheaodis-advantageaoofaousingaoaaowet-mountaoslide?ao-aoans--
Theaomovementaoisaoveryaofast,aomicrobesaoareaotransparent.aoMustaouseaoaao"dark-
fieldaostop".

Whataoisaoaaoproducer?ao-aoans--
Areaoautotrophaoorganismsaothataomakeaotheiraoownaofoodao(throughaophotosynthesis).