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Exam (elaborations)

Microbiology Lab Final Exam

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6 pages
2024/2025 Written for academic year
Institution Microbiology Lab
Course Microbiology Lab
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MICROBIOLOGY LAB FINAL EXAM

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MICROBIOLOGY LAB FINAL EXAM

Heataoreactionaotoao95aoCaotoaodenatureaoDNA
Coolaorxnaotoao55aoCaotoaoallowaoprimersaotoaoanneal
Heataorxnaotoao72aoCaotoaoallowaoTaqaopolymeraseaotoaobindao-aoans--
GiveaotheaostepsaoofaoaaoPCR

thermocyclerao-aoans--WhataomachineaoisaousedaotoaocarryaooutaoaaoPCRaoreaction?

30ao-aoans--HowaomanyaocyclesaodoesaoaaoPCRaorxnaousuallyaogo?

Bacillusaosubtillis
Enterobacteraoaerogenes
Enteroaocloacae
Escherichiaaocoli
Klebsiellaaopneumoniae
Pseudomonasaoaeruginosa
Salmonellaaotyphimurium
Staphylococcusaoaureus
Staphylococcusaoepidermidisao-aoans--
Nameaotheao9aoorganismsaousedaoinaotheaounknownaolab

E.aocoli
E.aocloacae
E.aoaerogenes
K.aopneumoniaao-aoans--Whichaoorganismsaocanaofermentaolactose?

rod-shaped,aoGram-negative,aonon-sporeaoforming,aoandaomotileaooraonon-
motileaobacteriaaowhichaocanaofermentaolactoseaowithaotheaoproductionaoofaoacidaoandaog
asao-aoans--Defineaocaliformaobacteria

BloodaoAgar
BileaoEsculin
MannitolaoSaltaoAgarao(MSA)
MacConkeyaoAgarao(MaC)
EosinaoMethyleneaoBlueao(EMB)
HektoenaoEntericaoAgarao(HEA)
XyloseaoLysineaoDesoxiolateao(XLD)ao-aoans--
Nameaotheaoselectiveaomediasaousedaoinaoclass

, O/FaoGlucose
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSIao-aoans--Nameaotheaodifferentialaomediasaousedaoinaoclass

indole
methylaored
voguesaoproskaver
in
citrateao-aoans--WhataodoesaotheaoacronymaoIMViCaostandaofor?

usedaotoaodifferentiateaogramaonegativeaorodsao-aoans--
WhataoisaotheaoIMViCaoseriesaoofaotestsaousedaofor?

Enterobacterao-aoans--
WhichaospecificaoorgnaismsaoareaotheaoIMViCaotestsaogoodaoataodifferentiating?

designedaotoaogrowaoonlyaospecificaobacteriaaobutaonotaoothersao-aoans--
Defineaoselectiveaomedia

designedaotoaoshowaotheaodifferenceaobetweenaodifferentaoorganismsaogrownaoonao-
aoans--Defineaodifferentialaomedia


showsaotheaotypesaoofaohemolysisao(abilityaotoaobreakaodownaoRBCs)aoofaoorganismsao-
aoans--Whataodoesaobloodaoagaraoshow?


alpha
beta
gammaao-aoans--Whataoareaotheao3aotypesaoofaohemolysis?

partialaogrowth,aogreenao-aoans--Describeaoalphaaogrowthaoonaobloodaoagar

totalaogrowth,aoclearao-aoans--Describeaobetaaogrowthaoonaobloodaoagar

nothing,aostillaoredao-aoans--Describeaogammaaogrowthaoonaobloodaoagar

abilityaoofaoorganismsaotoaotolerateaobileaoandaohydrolyzeaoesculinao-aoans--
Bileaoesculinaoshowsaowhat?

enterococciaoandaostreptococciao-aoans--
Whataotwoaotypesaoofaoorganismsaoisaobileaoesculinaogoodaoataodifferentiating?

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