K
BIO 351 FINAL EXAM
C
PREPARATION QUESTIONS
LO
AND CORRECT DETAILED /
YC
VERIFIED ANSWERS LATEST
UPDATE
D
U
ST
, You have made a transgene expressing a LacZ reporter gene. Where will this transgene
integrate into the genome? - ANS A random chromosomal location
You are able to fluorescently label cells in the ectoderm of a chick embryo and watch the
embryo over the course of a few days. You notice that the labeled cells now have developed
into cells shaped like... What best describes the process you have observed? - ANS
differentiation
which of the following properties of ES cells make them useful for making knock out mutations?
- ANS they are pluripotent, will self-renew, and will integrate vector DNA by homologous
K
recombination
Which of the following is the correct order of developmental steps leading to a specialized cell
C
type? - ANS specification, determination, differentiation
The diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
LO
through the ____ ligand on the inducing cell and ___ receptor o the responding cell in this
pathway. - ANS Delta, Notch
Which of the following techniques would be most appropriate for directly studying the secretion
of Wnt1 signaling and its distribution throughout the extracellular space of the embryonic spinal
YC
cord? - ANS In situ hybridization OR immunohistochemistry
Which statement is true? - ANS Bindin in necessary for sea urchin sperm to recognize eggs in
a species-specific manner.
differential gene expression is a central theme in development. which of the following
D
statements most accurately describes this concept? - ANS genomic DNA is the same in all
cell types, but mRNAs and proteins are different
U
Which of the following statements regarding conditional gene knockouts is false? - ANS the
targeting vector for inserting loxp sites utilizes nonhomologous recombination
ST
TRUE:
-Cre/loxp can be used to delete genetic material
-Cre expression is often under the control of a cell-specific promoter
-Cre recombinase is one of many known site-specific recombinases
as cells move along the continuum from stem cell to differentiated cell which of the following
correctly identifies the properties of a determined cell? - ANS can differentiate in a non-neutral
environment and its fate is irreversible
Which of the following statements is false? - ANS Surrounding cells must provide a cue for
specifying a particular cell fate.
BIO 351 FINAL EXAM
C
PREPARATION QUESTIONS
LO
AND CORRECT DETAILED /
YC
VERIFIED ANSWERS LATEST
UPDATE
D
U
ST
, You have made a transgene expressing a LacZ reporter gene. Where will this transgene
integrate into the genome? - ANS A random chromosomal location
You are able to fluorescently label cells in the ectoderm of a chick embryo and watch the
embryo over the course of a few days. You notice that the labeled cells now have developed
into cells shaped like... What best describes the process you have observed? - ANS
differentiation
which of the following properties of ES cells make them useful for making knock out mutations?
- ANS they are pluripotent, will self-renew, and will integrate vector DNA by homologous
K
recombination
Which of the following is the correct order of developmental steps leading to a specialized cell
C
type? - ANS specification, determination, differentiation
The diagram below shows an example of juxtacrine signaling. Cell-cell contact is mediated
LO
through the ____ ligand on the inducing cell and ___ receptor o the responding cell in this
pathway. - ANS Delta, Notch
Which of the following techniques would be most appropriate for directly studying the secretion
of Wnt1 signaling and its distribution throughout the extracellular space of the embryonic spinal
YC
cord? - ANS In situ hybridization OR immunohistochemistry
Which statement is true? - ANS Bindin in necessary for sea urchin sperm to recognize eggs in
a species-specific manner.
differential gene expression is a central theme in development. which of the following
D
statements most accurately describes this concept? - ANS genomic DNA is the same in all
cell types, but mRNAs and proteins are different
U
Which of the following statements regarding conditional gene knockouts is false? - ANS the
targeting vector for inserting loxp sites utilizes nonhomologous recombination
ST
TRUE:
-Cre/loxp can be used to delete genetic material
-Cre expression is often under the control of a cell-specific promoter
-Cre recombinase is one of many known site-specific recombinases
as cells move along the continuum from stem cell to differentiated cell which of the following
correctly identifies the properties of a determined cell? - ANS can differentiate in a non-neutral
environment and its fate is irreversible
Which of the following statements is false? - ANS Surrounding cells must provide a cue for
specifying a particular cell fate.
