BIO 351 FINAL EXAM PREPARATION QUESTIONS AND CORRECT DETAILED / VERIFIED ANSWERS LATEST UPDATE

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BIO 351 FINAL EXAM




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PREPARATION QUESTIONS
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AND CORRECT DETAILED /
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VERIFIED ANSWERS LATEST
UPDATE
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,What is the genomic signal that tells the cell to start transcription? - ANS promoter

What is the genomic signal that tells the cell to start translation? - ANS AUG start codon

Which of the following is an accurate statement about enhancers?
a) 5'-3' orientation is important for function
b) they are located 5' of the promoter
c) they are within 10 kb of the coding sequences
d) they regulate translational efficiency




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e) transcription factors bind to them - ANS transcription factors bind to them

myostatin (inhibits/promotes) myogenesis - ANS inhibits




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Which of the following is the correct order of developmental steps leading to a specialized cell
type?




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A. determination, differentiation, specification
B. differentiation, specification, determinati
C. differentiation, determination, specification
D. specification, determination, differentiation - ANS specification, determination,
differentiation
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Which of the following statements is FALSE?
A. Cell fates are reversible after specification.
B. During autonomous specification, cells require
communication with surrounding cells for commitment to
a fate.
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C. Differentiation involves changes in gene expression,
morphology, and function.
D. Cell fates are irreversible after determination - ANS False - During autonomous
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specification, cells require communication with surrounding cells for commitment to a fate

Asymmetric cell division describes which of the
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following?
A. Normal cell division.
B. A property of fully differentiated cells.
C. Meiosis
D. A property of stem cells that ensures self-
renewal. - ANS A property of stem cells that ensures self-
renewal

- A cell division that produces unequal daughter cells.

, Which of the following best describes the most important properties of the Yamanaka factors for
iPSC generation?
A. Viral proteins that induce pluripotency.
B. Signaling receptors in trophoblasts.
C. Multipotential
D. Pioneering transcription factors expressed in ES cells. - ANS Pioneering transcription
factors expressed in ES cells.

What is the expected result when siRNA is
used in a cell?




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A. It reduces expression of a specific protein.
B. It reduces transcription rate of a specific gene.
C. It directly binds to DNA in the nucleus.




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D. It enhances expression of a specific gene. - ANS It reduces expression of a specific
protein




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A plasmid is which of the following?
A. Circular extrachromosomal DNA
B. A chromosome
C. A protein
D. A bacteria - ANS Circular extrachromosomal DNA
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You are making a transgene, where do you
predict it will integrate into the genome?
A. At a specific gene locus.
B. A random chromosomal location
C. On a plasmid
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D. It does not integrate - ANS A random chromosomal location

Which of the following is a true statement about a
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transgenic organism?
1. A transgene is in all cells of an embryo.
2. A transgene is only in a subset of cells of
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the embryo.
3. A transgene is only in germ cells (eggs
and sperm).
4. A transgene is not found in cells - ANS A transgene is in all cells of an embryo.

You are making a knockout mouse, after neomycin (positive) selection you treat the surviving
cells with gancyclovir (negative selection) and more cells die. What does cell death after
gancyclovir treatment indicate?
a. The targeting vector randomly inserted into the genome
b. The targeting vector inserted into the genome by homologous recombination