K
BIO 351 FINAL EXAM
C
PREPARATION QUESTIONS
LO
AND CORRECT DETAILED /
YC
VERIFIED ANSWERS LATEST
UPDATE
D
U
ST
,A promoter is required for the initiation of which of the following processes? - ANS
Transcription
Why is the initially transcribed RNA precursor much larger than the mature mRNA? - ANS The
initial transcript contains introns, which are removed by splicing
Which of the following is the most accurate statement about enhancers? - ANS Transcription
factors bind to enhancers
Which of the following techniques would allow you to visualize gene expression in a living zebra
K
fish embryo, thus permitting repeated observations over time? - ANS Fluorescent transgenic
reporter
C
A transgene was constructed that mutated the MEF-2 site, but not the E1 site. The mutant
MEF-2 site transgene was expressed in all muscles except limbs. What would you conclude
about the MEF-2 site? - ANS It directs expression of the transgene in the limb muscle only
LO
If the MEF-2 site directs expression of the LacZ gene in certain skeletal muscles, what must it
bind to? - ANS Transcription factors
A transgenic mouse is not a chimera, why? - ANS Electroporation of vector DNA into ES cells
YC
results in an embryo in which all cells have the transgene
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's
genome? - ANS It will make cells sensitive to Glancyclovir
You want to knockout the Zombie gene in the brain only. What will allow you to do this
D
experiment? - ANS A mouse with a Cre recombinase that is expressed in the brain and a
mouse with a floxed Zombie allele
U
You have ES cells that have passed through double selection for your knockout experiment.
What is your next step? - ANS Inject the ES cells into a blastocyst
ST
During the period of limb bud outgrowth, a developmental biologist has transplanted some
mesenchyme from underneath the AER of a forelimb bud an moved it underneath the AER of
the hindlimb bud. What would you expect from this surgically altered limb bud? - ANS An
appendage that is proximal leg and distal wing
The__ is the morphogen expressed in the __ that caudalizes the limb bud - ANS SHH, zone of
polarizing activity
Which of the following terms describes the step at which cells are committed to a particular cell
fate, but it is still reversible? - ANS Specification
, A parasitic infection of the frog embryo has resulted in the following defect. The parasites
induced expression of __ and the formation of extra__. (Photo shows frog will multiple hind
limbs) - ANS FGF8, apical ectodermal ridge
Scientists have created a transgenic chick embryo that expresses Tbx5 in both the forelimb and
hindlimb bud regions but no Tbx4. What would you expect to see? - ANS A chicken with 4
legs
EMS cell normally divides and gives rise to cells that will form the mesoderm and endoderm. If
killing the P2 cells leads to the disruption of differentiation, which of the following best describes
K
the process taking place? - ANS Conditional specification of the EMS cell
The nematode Caenorhabditis elegans is an outstanding model for developmental genetics due
C
to several features. Which of the following is not a feature of this nematode? - ANS Every cell
in this nematode undergoes autonomous specification
LO
Spermatozoa that are harvested from the epididymis for IVF in men with blockage of their vas
deferens are incompetent to fertilize eggs. What must first happen for successful fertilization? -
ANS Capacitation
In sea urchins, the acrosome reaction: - ANS releases enzymes necessary for penetrating
YC
egg jelly
During cortical granule exocytosis, these granules release__ and this allows for the influx of __
and this causes the formation of the __. - ANS Serine proteases, H2O, fertilization envelope
You have made a mouse in which the Zp3 gene was knocked out. What is the likely phenotype?
D
- ANS The eggs cannot be fertilized because the sperm will not bind
WHich of the following terms describes the process by which the chromosomes of the egg and
U
those of the sperm combine just before the beginning of the first mitotic division? - ANS
Syngamy
ST
In humans, there is a genetic syndrome called situs inversus in which the internal organs are
located on the opposite sides than normal individuals. Which of the following structures is most
likely affected in development to produce this phenotype? - ANS Node
During gastrulation, cells of the epiblast migrate through__ to form the mesoderm and
endoderm - ANS Primitive streak
Which of the following produces right-left symmetry in the mouse embryo? - ANS
Unidirectional flow in nodal cilia
BIO 351 FINAL EXAM
C
PREPARATION QUESTIONS
LO
AND CORRECT DETAILED /
YC
VERIFIED ANSWERS LATEST
UPDATE
D
U
ST
,A promoter is required for the initiation of which of the following processes? - ANS
Transcription
Why is the initially transcribed RNA precursor much larger than the mature mRNA? - ANS The
initial transcript contains introns, which are removed by splicing
Which of the following is the most accurate statement about enhancers? - ANS Transcription
factors bind to enhancers
Which of the following techniques would allow you to visualize gene expression in a living zebra
K
fish embryo, thus permitting repeated observations over time? - ANS Fluorescent transgenic
reporter
C
A transgene was constructed that mutated the MEF-2 site, but not the E1 site. The mutant
MEF-2 site transgene was expressed in all muscles except limbs. What would you conclude
about the MEF-2 site? - ANS It directs expression of the transgene in the limb muscle only
LO
If the MEF-2 site directs expression of the LacZ gene in certain skeletal muscles, what must it
bind to? - ANS Transcription factors
A transgenic mouse is not a chimera, why? - ANS Electroporation of vector DNA into ES cells
YC
results in an embryo in which all cells have the transgene
What will be the result of thymidine kinase gene (TK) being incorporated into an ES cell's
genome? - ANS It will make cells sensitive to Glancyclovir
You want to knockout the Zombie gene in the brain only. What will allow you to do this
D
experiment? - ANS A mouse with a Cre recombinase that is expressed in the brain and a
mouse with a floxed Zombie allele
U
You have ES cells that have passed through double selection for your knockout experiment.
What is your next step? - ANS Inject the ES cells into a blastocyst
ST
During the period of limb bud outgrowth, a developmental biologist has transplanted some
mesenchyme from underneath the AER of a forelimb bud an moved it underneath the AER of
the hindlimb bud. What would you expect from this surgically altered limb bud? - ANS An
appendage that is proximal leg and distal wing
The__ is the morphogen expressed in the __ that caudalizes the limb bud - ANS SHH, zone of
polarizing activity
Which of the following terms describes the step at which cells are committed to a particular cell
fate, but it is still reversible? - ANS Specification
, A parasitic infection of the frog embryo has resulted in the following defect. The parasites
induced expression of __ and the formation of extra__. (Photo shows frog will multiple hind
limbs) - ANS FGF8, apical ectodermal ridge
Scientists have created a transgenic chick embryo that expresses Tbx5 in both the forelimb and
hindlimb bud regions but no Tbx4. What would you expect to see? - ANS A chicken with 4
legs
EMS cell normally divides and gives rise to cells that will form the mesoderm and endoderm. If
killing the P2 cells leads to the disruption of differentiation, which of the following best describes
K
the process taking place? - ANS Conditional specification of the EMS cell
The nematode Caenorhabditis elegans is an outstanding model for developmental genetics due
C
to several features. Which of the following is not a feature of this nematode? - ANS Every cell
in this nematode undergoes autonomous specification
LO
Spermatozoa that are harvested from the epididymis for IVF in men with blockage of their vas
deferens are incompetent to fertilize eggs. What must first happen for successful fertilization? -
ANS Capacitation
In sea urchins, the acrosome reaction: - ANS releases enzymes necessary for penetrating
YC
egg jelly
During cortical granule exocytosis, these granules release__ and this allows for the influx of __
and this causes the formation of the __. - ANS Serine proteases, H2O, fertilization envelope
You have made a mouse in which the Zp3 gene was knocked out. What is the likely phenotype?
D
- ANS The eggs cannot be fertilized because the sperm will not bind
WHich of the following terms describes the process by which the chromosomes of the egg and
U
those of the sperm combine just before the beginning of the first mitotic division? - ANS
Syngamy
ST
In humans, there is a genetic syndrome called situs inversus in which the internal organs are
located on the opposite sides than normal individuals. Which of the following structures is most
likely affected in development to produce this phenotype? - ANS Node
During gastrulation, cells of the epiblast migrate through__ to form the mesoderm and
endoderm - ANS Primitive streak
Which of the following produces right-left symmetry in the mouse embryo? - ANS
Unidirectional flow in nodal cilia
