BIOC 3021 Workshop 2 Questions and
Answers 100% Solved | Graded A+
Why does a plot of V vs S taper off and eventually reach a plateau at
higher S levels? - ✔✔- When S levels are high, the M-M plot actually
approaches a limiting value of Vmax.
- At high (S), where V approximates Vmax, virtually all of the enzyme active
sites are occupied by substrate.
- The addition of more S would not significantly increase the rate of
reaction, because the rate of reaction is limited by the total active sites
available; once all the sites are filled, the reaction can go no faster.
What is the Michaelis-Menten Equation? - ✔✔V = Vmax[S]/ [S] +km
The M-M Equation uses the terms (V), (Vmax), (S) and (Km). What do
each of these terms mean? - ✔✔V= observable velocity of the reaction.
Tells us how rapidly product (p) is being formed by the reaction.
Vmax = fastest reaction rate possible under the given assay conditions.
Has dimensions of change in product concentration per unit time.
S = substrate concentration
1
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, Km = Michaelis Constant. Has units of concentration
What does the Km term tell us about an enzyme? - ✔✔The Km tells us how
efficient enzymes are at low substrate levels
What does it signify if an enzyme has a low Km or a high Km? - ✔✔- The
Km tells us how efficient enzymes are at low substrate levels?
- At low Km, the enzyme is very efficient at low substrate concentration. At
high Km, enzymes are inefficient at low substrate concentration.
What is the enzyme rate when the concentration of S = Km? - ✔✔1/2
Vmax.
Why is a V vs S plot linear at low S concentrations? - ✔✔The M-M equation
reduces to V = K[S] which is the equation for a straight line.
This is b/c when Km is much greater than [S], the [S] term can be dropped
from the denominator
Why does the plot curve off at intermediate S levels - ✔✔Because the
whole M-M equation is used to describe the rate of the reaction.
Why does V vs S plot plateau at high S levels? - ✔✔The M-M equation
reduces to V=Vmax. When S levels are high, the M-M plot approaches a
limiting value of Vmax.
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
Answers 100% Solved | Graded A+
Why does a plot of V vs S taper off and eventually reach a plateau at
higher S levels? - ✔✔- When S levels are high, the M-M plot actually
approaches a limiting value of Vmax.
- At high (S), where V approximates Vmax, virtually all of the enzyme active
sites are occupied by substrate.
- The addition of more S would not significantly increase the rate of
reaction, because the rate of reaction is limited by the total active sites
available; once all the sites are filled, the reaction can go no faster.
What is the Michaelis-Menten Equation? - ✔✔V = Vmax[S]/ [S] +km
The M-M Equation uses the terms (V), (Vmax), (S) and (Km). What do
each of these terms mean? - ✔✔V= observable velocity of the reaction.
Tells us how rapidly product (p) is being formed by the reaction.
Vmax = fastest reaction rate possible under the given assay conditions.
Has dimensions of change in product concentration per unit time.
S = substrate concentration
1
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
, Km = Michaelis Constant. Has units of concentration
What does the Km term tell us about an enzyme? - ✔✔The Km tells us how
efficient enzymes are at low substrate levels
What does it signify if an enzyme has a low Km or a high Km? - ✔✔- The
Km tells us how efficient enzymes are at low substrate levels?
- At low Km, the enzyme is very efficient at low substrate concentration. At
high Km, enzymes are inefficient at low substrate concentration.
What is the enzyme rate when the concentration of S = Km? - ✔✔1/2
Vmax.
Why is a V vs S plot linear at low S concentrations? - ✔✔The M-M equation
reduces to V = K[S] which is the equation for a straight line.
This is b/c when Km is much greater than [S], the [S] term can be dropped
from the denominator
Why does the plot curve off at intermediate S levels - ✔✔Because the
whole M-M equation is used to describe the rate of the reaction.
Why does V vs S plot plateau at high S levels? - ✔✔The M-M equation
reduces to V=Vmax. When S levels are high, the M-M plot approaches a
limiting value of Vmax.
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
