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BIOC 3021 Exam Study Guide.
tRNA - answer✔Transfer RNA
Decodes mRNA into a protein
Functions at the ribosome during Translation
rRNA - answer✔Ribosomal RNA
Essential for protein synthesis
Predominant material in a ribosome (60%)
Catalyzes polypeptide formation (Ribozyme)
mRNA - answer✔Messanger RNA
Conveys genetic info from DNA to ribosome
Specifies the amino acid sequence of protein products
Which type of RNA catalyzes the synthesis of proteins? - answer✔rRNA
What enzyme complex makes DNA from RNA? - answer✔Reverse transcriptase
The first half of DNA restriction site has the sequence AACC, what are the next four bases on
the same chain? - answer✔GGTT
What percentage of human chromosomal DNA codes for proteins? - answer✔1-2%
DNA replication involves the nucleophilic attack on a deoxynucleotide phosphate by a: -
answer✔3' hydroxyl group of deoxyribose
What additional factor does lagging strand DNA synthesis require that is not needed for leading
strand synthesis? - answer✔a DNA ligase
Various forms of DNA that vary in the degree of winding are called: - answer✔Topoisomers
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If the first four bases are ATGC, what are the next four bases on the SAME DNA strand? -
answer✔GCAT
During DNA replication the lagging DNA strand is READ at its: - answer✔3' end
In addition to hydrogen bonding, the stability of double helix between complementary DNA
chains is promoted by? - answer✔Aromatic stacking
In the structure of ATP the inner phosphate group is joined to the 5' carbon of ribose by what
type of bond? - answer✔Phosphoester
What feature is found in DNA synthesis but NOT in RNA synthesis? - answer✔Repair
mechanisms which remove incorrect bases
Which statement is TRUE about ribosomes? - answer✔Protein synthesis is catalyzed by
ribosomal RNA
The process of cutting DNA strands at specific sites is accomplished by? - answer✔Restriction
endonucleases
Which of the following is needed for replication of DNA but NOT for transcription of DNA to
RNA? - answer✔a primer strand
In bacteria a protein with 1200 amino acids might be coded by a sequence of DNA with an open
reading frame of - answer✔3600 bases (3 for each AA)
Which of the following statements is TRUE about the coding strand of DNA? - answer✔It has
the same base sequence as the transcript except for the substitution of U's
The signals at the ends of introns in immature mRNA, that code for their removal are: -
answer✔GU and AG
If a restriction site in a circular plasmid has the sequence GGAATTCC, and the endonucleas cuts
between A and A, how many unpaired bases will occur on each strand at the opening that is
produced? - answer✔2
In RNA synthesis to which base in the DNA is the U in the RNA product paired? - answer✔A
At neutral pH, DNA carries a negative charge because of the - answer✔Phosphate
The critical ingredient in PCR which allows for multiple rounds of DNA synthesis is? -
answer✔The heat resistant DNA polymerase
The hydrolytic enzymes, used in cloning experiments, which make cuts at distinct sites in DNA
are called? - answer✔restriction endonucleases
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