©Themoon EXAM SOLUTIONS
25/11/2024 09:27AM
BIOC 301 Midterm Exam and Answers
B-galactosidase
lactose
galactose
glucose - Answers✓✓LacZ codes for ________ which hydrolyzes _______ into ______
and_____
chaotropes - Answers✓✓small molecules used to denature proteins (urea, guanidinium
chloride); bind to water and reduce hydrophobic effect
Melting temperature formula will be given if we need it, but how do you calculate the
annealing temperature if you have the melting temperature - Answers✓✓subtract between 2-5
degrees from the melting temperature
Taq Polymerase can add _______bp/min - Answers✓✓1000
_________ is a cofactor for Taq - Answers✓✓magnesium
What replaces helicase in PCR? - Answers✓✓Heat
primers bind to the _____ end of DNA strands - Answers✓✓3' end
Longer primers require ________ melting temperatures which can prevent..... -
Answers✓✓higher
, ©Themoon EXAM SOLUTIONS
25/11/2024 09:27AM
mispriming
In order to do PCR you need _____,_____, and _____ - Answers✓✓dNTP
Primers
Polymerase
Which nucleotide bond is stronger and why? - Answers✓✓G-C because it forms 3 H-bonds
whereas A-T only forms 2 H-bonds
How long is the LacZ gene? - Answers✓✓~3000bp
Optimal prier is around ____bp and has a melting point between ____-____degrees celsius -
Answers✓✓20
55-65
Total cation concentration for calculating Tm is usually _____ - Answers✓✓50mM
How do you prevent a plasmid from reattaching to itself after it has been cut using restriction
enzymes? - Answers✓✓You dephosphorylate the sticky ends of the cut plasmid using alkaline
phosphatase.
This involves removing the terminal phosphate groups of the DNA so that no phosphate is
available.
If you increase the % agarose of a gel tray then the DNA will migrate ______ -
Answers✓✓slower
25/11/2024 09:27AM
BIOC 301 Midterm Exam and Answers
B-galactosidase
lactose
galactose
glucose - Answers✓✓LacZ codes for ________ which hydrolyzes _______ into ______
and_____
chaotropes - Answers✓✓small molecules used to denature proteins (urea, guanidinium
chloride); bind to water and reduce hydrophobic effect
Melting temperature formula will be given if we need it, but how do you calculate the
annealing temperature if you have the melting temperature - Answers✓✓subtract between 2-5
degrees from the melting temperature
Taq Polymerase can add _______bp/min - Answers✓✓1000
_________ is a cofactor for Taq - Answers✓✓magnesium
What replaces helicase in PCR? - Answers✓✓Heat
primers bind to the _____ end of DNA strands - Answers✓✓3' end
Longer primers require ________ melting temperatures which can prevent..... -
Answers✓✓higher
, ©Themoon EXAM SOLUTIONS
25/11/2024 09:27AM
mispriming
In order to do PCR you need _____,_____, and _____ - Answers✓✓dNTP
Primers
Polymerase
Which nucleotide bond is stronger and why? - Answers✓✓G-C because it forms 3 H-bonds
whereas A-T only forms 2 H-bonds
How long is the LacZ gene? - Answers✓✓~3000bp
Optimal prier is around ____bp and has a melting point between ____-____degrees celsius -
Answers✓✓20
55-65
Total cation concentration for calculating Tm is usually _____ - Answers✓✓50mM
How do you prevent a plasmid from reattaching to itself after it has been cut using restriction
enzymes? - Answers✓✓You dephosphorylate the sticky ends of the cut plasmid using alkaline
phosphatase.
This involves removing the terminal phosphate groups of the DNA so that no phosphate is
available.
If you increase the % agarose of a gel tray then the DNA will migrate ______ -
Answers✓✓slower
