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Microbiology LAB UTC - Barbosa Exam Questions And Improved Responses

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Microbiology LAB UTC - Barbosa Exam Questions And Improved Responses What does MSA stand for? - answersMannitol Salt Agar What does MAC stand for? - answersMacConkey's Agar What does EMB stand for? - answersEosin Methylene Blue Agar How is the MSA test selective, and what type of bacteria growth does it NOT inhibit (allows to grow)? - answersIt contains mannitol (sugar) which is used for fermentation The high salt concentration inhibits the growth of most bacteria other than staphylococci How is the MSA test differential? - answersthe phenol red is a pH indicator that changes from red to yellow when the bacteria ferments the mannitol and produces an acid On the MSA test, which bacteria shows growth AND produces a color change? - answersStaphylococcus aureus On the MSA test, which bacteria produces colonies but does NOT produce a color change? - answersStaphylococcus epidermis How is the MAC test selective, and which type of bacteria does it inhibit growth? - answersThe crystal violet and bile salts inhibit the growth of gram-Positive organisms The lactose in the agar allows for fermentation How is the MAC test differential? - answersThe lactose allows for fermentation, whose acid end-products act on bile salts, and neutral red is absorbed by the precipitated salts.

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Microbiology LAB UTC 4

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©JUSTTRACY EXAM SOLUTIONS
21/11/202410:20AM

Microbiology LAB UTC - Barbosa Exam
Questions And Improved Responses
What does MSA stand for? - answers✔✔Mannitol Salt Agar

What does MAC stand for? - answers✔✔MacConkey's Agar

What does EMB stand for? - answers✔✔Eosin Methylene Blue Agar

How is the MSA test selective, and what type of bacteria growth does it NOT inhibit (allows
to grow)? - answers✔✔It contains mannitol (sugar) which is used for fermentation

The high salt concentration inhibits the growth of most bacteria other than staphylococci

How is the MSA test differential? - answers✔✔the phenol red is a pH indicator that changes
from red to yellow when the bacteria ferments the mannitol and produces an acid

On the MSA test, which bacteria shows growth AND produces a color change? -
answers✔✔Staphylococcus aureus

On the MSA test, which bacteria produces colonies but does NOT produce a color change? -
answers✔✔Staphylococcus epidermis

How is the MAC test selective, and which type of bacteria does it inhibit growth? -
answers✔✔The crystal violet and bile salts inhibit the growth of gram-Positive organisms

The lactose in the agar allows for fermentation

How is the MAC test differential? - answers✔✔The lactose allows for fermentation, whose
acid end-products act on bile salts, and neutral red is absorbed by the precipitated salts.

, ©JUSTTRACY EXAM SOLUTIONS
21/11/202410:20AM

Non-fermenting bacteria give an alkaline reaction and are non colored/transparent.

On the MAC test, which bacteria produced red/pink colonies? - answers✔✔Escherichia coli

On the MAC test, which bacteria produced colorless colonies? - answers✔✔Salmonella
enteritidis

How is the EMB test selective? - answers✔✔The methylene blue acts as an inhibitor to gram-
Positive organisms.

Both lactose and saccharose are included to allow for fermentation.

How is the EMB test differential? - answers✔✔INDICATOR for those that ferment lactose
and those that do not... eosin & methylene blue

Strong lactose fermentation = green metallic sheen

Weak lactose fermentation/ prefer saccharose = pinkish colonies, mucoid, larger colonies

No fermentation = colorless

On the EMB test, which bacteria produced a green metallic sheen? - answers✔✔Escherichia
coli

On the EMB test, which bacteria produced pinkish colonies? - answers✔✔Enterobacter
aerogenes

On the EMB test, which bacteria were colorless? - answers✔✔Salmonella enteritidis

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