BIOL 2051 LAB FINAL UPDATED
ACTUAL Questions and CORRECT
Answers
enzymes - CORRECT ANSWER✔✔- proteins that serve as biological catalysts
substrate - CORRECT ANSWER✔✔- substance that reacts with an enzyme
one - CORRECT ANSWER✔✔- generally, how many substrates may an enzyme react with?
extracellular enzymes - CORRECT ANSWER✔✔- enzymes that act outside of the cell in
which they are produced
degrade large molecules into units that are small enough to be transported by proteins across
the cell membrane and into the cell where they may be used - CORRECT ANSWER✔✔-
Generally, how are extracellular enzymes useful?
endoenzymes - CORRECT ANSWER✔✔- act inside of the cell to synthesize molecules that
are needed by the cell OR degrade molecules to simpler molecules, releasing energy
inducible - CORRECT ANSWER✔✔- type of enzyme that is only produced when a
substrate is available
constitutive - CORRECT ANSWER✔✔- type of enzyme that are essential to the cell and
produced at all times
morphology - CORRECT ANSWER✔✔- cell size, shape, and arrangement of a bacterium
physiology - CORRECT ANSWER✔✔- how an organism functions in an environment; its
metabolic capabilities.. enzymes produced and biochemical reactions
,oxidase and catalase - CORRECT ANSWER✔✔- What two enzymes are made by organisms
that produce energy using aerobic respiration?
molecular oxygen - CORRECT ANSWER✔✔- in aerobic respiration, what serves as the
final electron acceptor of the electron transport chain
cytochrome oxidase - CORRECT ANSWER✔✔- oxygen accepts an electron from what
protein in the electron transport chain during aerobic respiration?
cytochrome c oxidase - CORRECT ANSWER✔✔- What is tested for in an oxidase assay?
facultative aerobes - CORRECT ANSWER✔✔- Many oxidase negative organisms are what
in terms of oxygen use?
toxic byproducts - CORRECT ANSWER✔✔- What are produced by the reduction of
oxygen?
B - CORRECT ANSWER✔✔- An organism's catalase production gives you information
about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
C) the presence of nitrate reductase in the cell
D) fermentation properties
true - CORRECT ANSWER✔✔- T/F
a microscope slide is a convenient surface to perform a catalase test
B - CORRECT ANSWER✔✔- Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation
,true - CORRECT ANSWER✔✔- T/F
Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome oxidase for
aerobic respiration
true - CORRECT ANSWER✔✔- T/F
Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic respiration
true - CORRECT ANSWER✔✔- T/F
dark-dier mutant of C. reinhardtii can grow on minimal media
false - CORRECT ANSWER✔✔- T/F
dark-dier mutant of C. reinhardtii is an anoxygenic phototroph
B - CORRECT ANSWER✔✔- Which of the following describes how you will inoculate an
HA plate to test the effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on the other
side
C) streak plate
D) pour plate
true - CORRECT ANSWER✔✔- T/F
denitrification by soil microbes depletes the soil of nitrogen
A - CORRECT ANSWER✔✔- Which of the following are inoculated to test for nitrate
reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
, D) nitrite broth
false - CORRECT ANSWER✔✔- T/F
To perform the catalase test, add a drop of water to the slide to mix with the bacteria
molecular oxygen serves as the electron acceptor - CORRECT ANSWER✔✔- How is
aerobic respiration distinguished form anaerobic respiration?
false - CORRECT ANSWER✔✔- T/F
wild-type C. reinhardtii cannot use acetate in the dark for aerobic respiration
true - CORRECT ANSWER✔✔- T/F
an organism is doing anaerobic respiration if it uses nitrate at the end of the electron transport
chain as the final electron acceptor
false - CORRECT ANSWER✔✔- T/F
the positive control tube for nitrate reduction is inoculated with both E. Coli and P.
denitrificans
microscope slide - CORRECT ANSWER✔✔- on what surface will you be placing the
bacteria for the catalase test in lab?
false - CORRECT ANSWER✔✔- T/F
oxidase enzymes are made by organisms that produce energy using anaerobic respiration
false - CORRECT ANSWER✔✔- T/F
cytochrome oxidase is an enzyme that can catalyze the reduction of a cytochrome and the
subsequent oxidation of molecular oxygen to either water or hydrogen peroxide
true - CORRECT ANSWER✔✔- T/F
organisms from the genera Pseudomonas are considered oxidase positive
ACTUAL Questions and CORRECT
Answers
enzymes - CORRECT ANSWER✔✔- proteins that serve as biological catalysts
substrate - CORRECT ANSWER✔✔- substance that reacts with an enzyme
one - CORRECT ANSWER✔✔- generally, how many substrates may an enzyme react with?
extracellular enzymes - CORRECT ANSWER✔✔- enzymes that act outside of the cell in
which they are produced
degrade large molecules into units that are small enough to be transported by proteins across
the cell membrane and into the cell where they may be used - CORRECT ANSWER✔✔-
Generally, how are extracellular enzymes useful?
endoenzymes - CORRECT ANSWER✔✔- act inside of the cell to synthesize molecules that
are needed by the cell OR degrade molecules to simpler molecules, releasing energy
inducible - CORRECT ANSWER✔✔- type of enzyme that is only produced when a
substrate is available
constitutive - CORRECT ANSWER✔✔- type of enzyme that are essential to the cell and
produced at all times
morphology - CORRECT ANSWER✔✔- cell size, shape, and arrangement of a bacterium
physiology - CORRECT ANSWER✔✔- how an organism functions in an environment; its
metabolic capabilities.. enzymes produced and biochemical reactions
,oxidase and catalase - CORRECT ANSWER✔✔- What two enzymes are made by organisms
that produce energy using aerobic respiration?
molecular oxygen - CORRECT ANSWER✔✔- in aerobic respiration, what serves as the
final electron acceptor of the electron transport chain
cytochrome oxidase - CORRECT ANSWER✔✔- oxygen accepts an electron from what
protein in the electron transport chain during aerobic respiration?
cytochrome c oxidase - CORRECT ANSWER✔✔- What is tested for in an oxidase assay?
facultative aerobes - CORRECT ANSWER✔✔- Many oxidase negative organisms are what
in terms of oxygen use?
toxic byproducts - CORRECT ANSWER✔✔- What are produced by the reduction of
oxygen?
B - CORRECT ANSWER✔✔- An organism's catalase production gives you information
about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
C) the presence of nitrate reductase in the cell
D) fermentation properties
true - CORRECT ANSWER✔✔- T/F
a microscope slide is a convenient surface to perform a catalase test
B - CORRECT ANSWER✔✔- Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation
,true - CORRECT ANSWER✔✔- T/F
Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome oxidase for
aerobic respiration
true - CORRECT ANSWER✔✔- T/F
Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic respiration
true - CORRECT ANSWER✔✔- T/F
dark-dier mutant of C. reinhardtii can grow on minimal media
false - CORRECT ANSWER✔✔- T/F
dark-dier mutant of C. reinhardtii is an anoxygenic phototroph
B - CORRECT ANSWER✔✔- Which of the following describes how you will inoculate an
HA plate to test the effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on the other
side
C) streak plate
D) pour plate
true - CORRECT ANSWER✔✔- T/F
denitrification by soil microbes depletes the soil of nitrogen
A - CORRECT ANSWER✔✔- Which of the following are inoculated to test for nitrate
reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
, D) nitrite broth
false - CORRECT ANSWER✔✔- T/F
To perform the catalase test, add a drop of water to the slide to mix with the bacteria
molecular oxygen serves as the electron acceptor - CORRECT ANSWER✔✔- How is
aerobic respiration distinguished form anaerobic respiration?
false - CORRECT ANSWER✔✔- T/F
wild-type C. reinhardtii cannot use acetate in the dark for aerobic respiration
true - CORRECT ANSWER✔✔- T/F
an organism is doing anaerobic respiration if it uses nitrate at the end of the electron transport
chain as the final electron acceptor
false - CORRECT ANSWER✔✔- T/F
the positive control tube for nitrate reduction is inoculated with both E. Coli and P.
denitrificans
microscope slide - CORRECT ANSWER✔✔- on what surface will you be placing the
bacteria for the catalase test in lab?
false - CORRECT ANSWER✔✔- T/F
oxidase enzymes are made by organisms that produce energy using anaerobic respiration
false - CORRECT ANSWER✔✔- T/F
cytochrome oxidase is an enzyme that can catalyze the reduction of a cytochrome and the
subsequent oxidation of molecular oxygen to either water or hydrogen peroxide
true - CORRECT ANSWER✔✔- T/F
organisms from the genera Pseudomonas are considered oxidase positive
