©BRIGHTSTARS EXAM SOLUTIONS
11/16/2024 10:16 AM
MIC224 Midterm Exam Questions With
Correct Answers
Ocular - answer✔moves left/right to adjust interpupillary distance
Binocular tube - answer✔swivels to permit eyepiece housing to rotate.
Stage adjustment knobs - answer✔used to move the slide on a mechanical stage in vertical and
horizontal directions
Lamp intensity Dial - answer✔used to adjust light intensity
Revolving nose piece - answer✔attached to objective lenses and rotates around
objective lenses - answer✔magnification that starts at 4x goes to 10x, 40x, then 100x.
Condenser - answer✔used to adjust both intensity and contrast of light.
Total magnification - answer✔Multiply ocular lens (eyepiece) by the objective lens
resolution - answer✔is the ability to discern the fine detail. Just making an image larger does
not make the ability to see it better.
Light capturing ability - answer✔what creates resolution. Microscopes are specifically
manufactured to capture the greatest amount of light even at high magnifications.
The 100x oil immersion lens - answer✔is designed to utilize a small drop of oil that has the
same refractive index as glass. The light is "fooled" into thinking that it has not left the slide and
enters into the lens in a strait line minimizing loses. You cannot focus this lens without oil.
Ocular scale - answer✔Identify
interpupillary distance - answer✔distance between eyepieces
Arm of microscope - answer✔9used to pick up the mictoscope
1|Page
, ©BRIGHTSTARS EXAM SOLUTIONS
11/16/2024 10:16 AM
condenser height adjustment knob - answer✔used to raise and lower condenser houseing.
Adjust light intensity enetering objective lenses. Should be adjusted so that light does not blind
viewer, yet illuminate specimen fully..
coarse focus knob - answer✔moves stage height in large increments. Used only for finding and
initially focusing on specimen using low power lenses only.
Fine adjustment knob - answer✔moves stage height in fine increments. Used with lower power
lenses for final focus. Must be used in 40x and 100x
Ubiquitous - answer✔found everywhere; microbes are this.
Mircroorganisms - answer✔too small to look at with the naked eye.
How to look at microorganisms - answer✔grow the organism and then stain, use microscope to
look at after.
How we grow microorganisms - answer✔with the use of agar
Agar - answer✔a gel-like polysaccharide compound used for culturing microbes; extracted from
certain red algae
Solid media - answer✔made of agar
Liquid media - answer✔made of broth
Stab - answer✔another wat to see solid media. Were solid media is put in a test tube and then
an inoculating needle is stabbed through with the organism on it. Sometimes call ed a deep.
Slant form - answer✔agar is placed in a test tub and before it drys, we slant the tube to give a
nice flat surface to place bacteria on. Easier to store.
PCA - answer✔Plate Count Agar: general purpose media. Used for swapping desk or show and
then placed on media.
PDA - answer✔Potato dextrose agar. Selective media used to grow yeast and molds (fungi) -
this is the one we left on our desk for the air to contaminate.
Each group of microorganism on a media - answer✔not individual cells, but colonies
Mold and fungi - answer✔give off hair like projections called filaments growth. That is the main
difference on how to tell them apart.
2|Page
11/16/2024 10:16 AM
MIC224 Midterm Exam Questions With
Correct Answers
Ocular - answer✔moves left/right to adjust interpupillary distance
Binocular tube - answer✔swivels to permit eyepiece housing to rotate.
Stage adjustment knobs - answer✔used to move the slide on a mechanical stage in vertical and
horizontal directions
Lamp intensity Dial - answer✔used to adjust light intensity
Revolving nose piece - answer✔attached to objective lenses and rotates around
objective lenses - answer✔magnification that starts at 4x goes to 10x, 40x, then 100x.
Condenser - answer✔used to adjust both intensity and contrast of light.
Total magnification - answer✔Multiply ocular lens (eyepiece) by the objective lens
resolution - answer✔is the ability to discern the fine detail. Just making an image larger does
not make the ability to see it better.
Light capturing ability - answer✔what creates resolution. Microscopes are specifically
manufactured to capture the greatest amount of light even at high magnifications.
The 100x oil immersion lens - answer✔is designed to utilize a small drop of oil that has the
same refractive index as glass. The light is "fooled" into thinking that it has not left the slide and
enters into the lens in a strait line minimizing loses. You cannot focus this lens without oil.
Ocular scale - answer✔Identify
interpupillary distance - answer✔distance between eyepieces
Arm of microscope - answer✔9used to pick up the mictoscope
1|Page
, ©BRIGHTSTARS EXAM SOLUTIONS
11/16/2024 10:16 AM
condenser height adjustment knob - answer✔used to raise and lower condenser houseing.
Adjust light intensity enetering objective lenses. Should be adjusted so that light does not blind
viewer, yet illuminate specimen fully..
coarse focus knob - answer✔moves stage height in large increments. Used only for finding and
initially focusing on specimen using low power lenses only.
Fine adjustment knob - answer✔moves stage height in fine increments. Used with lower power
lenses for final focus. Must be used in 40x and 100x
Ubiquitous - answer✔found everywhere; microbes are this.
Mircroorganisms - answer✔too small to look at with the naked eye.
How to look at microorganisms - answer✔grow the organism and then stain, use microscope to
look at after.
How we grow microorganisms - answer✔with the use of agar
Agar - answer✔a gel-like polysaccharide compound used for culturing microbes; extracted from
certain red algae
Solid media - answer✔made of agar
Liquid media - answer✔made of broth
Stab - answer✔another wat to see solid media. Were solid media is put in a test tube and then
an inoculating needle is stabbed through with the organism on it. Sometimes call ed a deep.
Slant form - answer✔agar is placed in a test tub and before it drys, we slant the tube to give a
nice flat surface to place bacteria on. Easier to store.
PCA - answer✔Plate Count Agar: general purpose media. Used for swapping desk or show and
then placed on media.
PDA - answer✔Potato dextrose agar. Selective media used to grow yeast and molds (fungi) -
this is the one we left on our desk for the air to contaminate.
Each group of microorganism on a media - answer✔not individual cells, but colonies
Mold and fungi - answer✔give off hair like projections called filaments growth. That is the main
difference on how to tell them apart.
2|Page
