Micro Lab Quiz 3/ Actual Exam Questions
with Correct Verified Answers/ Graded A+
what tests would you perform for identifying a urine specimen - Correct Answer - 1.
gram stain
2. oxidase spot test
3. indole spot test
what does the oxidase test test for? - Correct Answer - indophenol oxidase-an enzyme
produced by an organism
signs of a possible kidney infection include: (4) - Correct Answer - -increased protein
-blood present
-nitrate positive
-leukocyte esterase positive
urine culture for bacteria should be plated to... - Correct Answer - SBA/ BAP
MAC
...in order to isolate pathogens
when is a stool specimen usually ordered? - Correct Answer - If a patient has diarrhea
for long period of time or is extremely ill
How should a stool specimen be collected? - Correct Answer - In a clean container,
does not have to be sterile (specimens are usually not formed (diarrhea, watery, bloody,
mucous)
pg. 1
, How should a stool specimen be processed and transported? - Correct Answer - -
processed within 2 hrs.
-refrigerated if delayed
-can use Cary Blair media for transport
What should be done if a parasite is suspected in a stool sample? - Correct Answer -
order an ova and parasite exam or screen for common parasites like Giardia and
Cryptosporidium
common causes of Salmonella, Shigella, Yersinia and Campylobacter - Correct Answer
- food/water poisoning...not normal flora of bowel
What biochemical tests should be performed on all isolates for stool samples - Correct
Answer - 1. Triple Sugar Iron salt slant
2. Urea broth or Urea slant
3. MRVP broth
4. Citrate slant
5. subculture to SBA/BAP for gram stain, oxidase, and indole
TSI test - Correct Answer - Triple Sugar Iron Agar
What does the Indole spot test for? - Correct Answer - tryptophanase- an enzyme that
produces indole in the presence of the amino acid tryptophan (in SBA)
reagents in Indole test - Correct Answer - p-dimethylaminocinnamaldehyde
Indole test procedure - Correct Answer - 1. drop reagent on filter paper
2. smear organism on over reagent
3. green on filter paper if positive
pg. 2
with Correct Verified Answers/ Graded A+
what tests would you perform for identifying a urine specimen - Correct Answer - 1.
gram stain
2. oxidase spot test
3. indole spot test
what does the oxidase test test for? - Correct Answer - indophenol oxidase-an enzyme
produced by an organism
signs of a possible kidney infection include: (4) - Correct Answer - -increased protein
-blood present
-nitrate positive
-leukocyte esterase positive
urine culture for bacteria should be plated to... - Correct Answer - SBA/ BAP
MAC
...in order to isolate pathogens
when is a stool specimen usually ordered? - Correct Answer - If a patient has diarrhea
for long period of time or is extremely ill
How should a stool specimen be collected? - Correct Answer - In a clean container,
does not have to be sterile (specimens are usually not formed (diarrhea, watery, bloody,
mucous)
pg. 1
, How should a stool specimen be processed and transported? - Correct Answer - -
processed within 2 hrs.
-refrigerated if delayed
-can use Cary Blair media for transport
What should be done if a parasite is suspected in a stool sample? - Correct Answer -
order an ova and parasite exam or screen for common parasites like Giardia and
Cryptosporidium
common causes of Salmonella, Shigella, Yersinia and Campylobacter - Correct Answer
- food/water poisoning...not normal flora of bowel
What biochemical tests should be performed on all isolates for stool samples - Correct
Answer - 1. Triple Sugar Iron salt slant
2. Urea broth or Urea slant
3. MRVP broth
4. Citrate slant
5. subculture to SBA/BAP for gram stain, oxidase, and indole
TSI test - Correct Answer - Triple Sugar Iron Agar
What does the Indole spot test for? - Correct Answer - tryptophanase- an enzyme that
produces indole in the presence of the amino acid tryptophan (in SBA)
reagents in Indole test - Correct Answer - p-dimethylaminocinnamaldehyde
Indole test procedure - Correct Answer - 1. drop reagent on filter paper
2. smear organism on over reagent
3. green on filter paper if positive
pg. 2
