MCB3020 Final Exam Study Guide
Questions With 100% Pass
virus - Answer-An infectious agent made up of protein, nucleic acids and sometimes a
lipid membrane
/.Viroid - Answer-An infectious agent consisting of only RNA
/.Prion - Answer-An infectious agent consisting of only protein
/.The proper sequence of reagents in the Gram stain procedure is - Answer-crystal
violet, iodine, ethanol, safranin.
/.The Gram stains of of a bacterial cell is based on the chemical properties of its -
Answer-cell wall
/.True or False: The Gram stain can only be performed on cultures grown in liquid
media. - Answer-False
/.After performing a Gram stain of a mixed culture of Gram-positive and Gram-negative
cells, you shout: "Oops, I forgot the iodine step!" What would you expect to see if you
observed the slide under the microscope? - Answer-Pink Gram-positive and pink Gram-
negative cells
/.Acetone can be used as the decolorizer in the Gram stain procedure. - Answer-true
/.Storage inclusion - Answer-stores nutrients, energy, metabolic products, building
blocks
-carbon
-glycogen
-amino acids
/.Chemotaxis - Answer-the process by which bacteria move toward an attractant or
away from a repellent
/.What is the most common cell wall in most Archaea? - Answer-S layer
/.Antiseptic surgery was pioneered by - Answer-Lister
/.Which of the following is not true about archaeal ribosomes? - Answer-They have
similar antibiotic sensitivities to those of bacteria.
/.Unlike members of the Bacteria, most archaeans have linear chromosomes. - Answer-
false
,/.The concept that living cells can arise from nonliving material - Answer-spontaneous
generation
/.Golgi apparatus - Answer-Involved in the modification, packaging, and secretion of
materials
/.Rough ER - Answer-ER that is dotted with ribosomes and where protein synthesis
occur
/.The nucleolus plays a major role in synthesis of - Answer-rRNA
/.The first of Koch's postulates is - Answer-the microorganism must be present in every
case of the disease but absent from healthy organisms.
/.Which of the following bacterial structures are most directly linked to swimming motility
in chemotaxis? - Answer-flagella
/.True or False: Gram-positive bacteria have a thinner layer of peptidoglycan than gram-
negative bacteria - Answer-False
/.Cytosol - Answer-liquid component of the cytoplasm
/.What is useful in distinguishing between prokaryotic and eukaryotic cells? - Answer-
The presence of membrane-delimited organelles within the cytoplasm
/.ER - Answer-Location for protein synthesis and some protein modifications
/.which of the following advances best describes that today we maybe experiencing a
second golden age of microbiology? - Answer-genomic testing
/.Perisomes - Answer-Location for storage of protective compounds that break down
lipids and detoxify certain chemicals
/.function of mitochondria - Answer-tricarboxylic acid cycle enzyme reaction, electron
transport, ATP synthesis
/.Lacks linear chromosome and has a peptidoglycan cell wall - Answer-Bacteria
/.Which of the following describes an organism that rarely contains plasmid? - Answer-
Eukarya
/.Which acellullar agent/s are composed of nucleic acids only? Check all that apply. -
Answer-satellite, prion, virus
, /.Which of the following describes an organism that contains 80S ribosomes? - Answer-
eukarya
/.An external structure unique to archaea are the ______. - Answer-cannulae
/.If you found a microbe with an S-layer as the major component of its cell wall, how
would you identify this microbe? - Answer-Archaeon
/.Cell wall of bacteria is composed of - Answer-Cellulose and peptidoglycan
/.Lysosomes - Answer-Location for storage of many types of degradative digestive
enzymes. Arise from the Golgi apparatus. Contains hydrolytic enzymes.
/.The plasma membrane of eukaryotes contains all of the following EXCEPT - Answer-
peptidoglycan.
/.Which of the following structures are external to both Bacterial and Archaeal cells? -
Answer-pilli
/.Bacillus - Answer-rod-shaped
/.If you remove the peptidoglycan layer from a Gram-positive cell, it would still stain
purple with a Gram stain. - Answer-false
/.External structures that are found in Archaea but not Bacteria are _________. -
Answer-hami
/.periplasmic space - Answer-only found in gram-positive bacteria
/.Which of the following is NOT a function of the capsule? - Answer-used for regulating
nutrient uptake
/.All of the following statements about the periplasmic space are true except: - Answer-It
is found only in gram-positive bacteria.
/.Flexible bacteria with a helical shape are called - Answer-spirochetes.
/.Which of the following lacks linear chromosomes and peptidoglycan cell walls? -
Answer-Archaea
/.Who of the following provided evidence that contributed to discredit the concept of
spontaneous generation? - Answer-Pasteur
/.Lysosomes are membrane-bound vesicles that arise from the __________. - Answer-
golgi apparatus
Questions With 100% Pass
virus - Answer-An infectious agent made up of protein, nucleic acids and sometimes a
lipid membrane
/.Viroid - Answer-An infectious agent consisting of only RNA
/.Prion - Answer-An infectious agent consisting of only protein
/.The proper sequence of reagents in the Gram stain procedure is - Answer-crystal
violet, iodine, ethanol, safranin.
/.The Gram stains of of a bacterial cell is based on the chemical properties of its -
Answer-cell wall
/.True or False: The Gram stain can only be performed on cultures grown in liquid
media. - Answer-False
/.After performing a Gram stain of a mixed culture of Gram-positive and Gram-negative
cells, you shout: "Oops, I forgot the iodine step!" What would you expect to see if you
observed the slide under the microscope? - Answer-Pink Gram-positive and pink Gram-
negative cells
/.Acetone can be used as the decolorizer in the Gram stain procedure. - Answer-true
/.Storage inclusion - Answer-stores nutrients, energy, metabolic products, building
blocks
-carbon
-glycogen
-amino acids
/.Chemotaxis - Answer-the process by which bacteria move toward an attractant or
away from a repellent
/.What is the most common cell wall in most Archaea? - Answer-S layer
/.Antiseptic surgery was pioneered by - Answer-Lister
/.Which of the following is not true about archaeal ribosomes? - Answer-They have
similar antibiotic sensitivities to those of bacteria.
/.Unlike members of the Bacteria, most archaeans have linear chromosomes. - Answer-
false
,/.The concept that living cells can arise from nonliving material - Answer-spontaneous
generation
/.Golgi apparatus - Answer-Involved in the modification, packaging, and secretion of
materials
/.Rough ER - Answer-ER that is dotted with ribosomes and where protein synthesis
occur
/.The nucleolus plays a major role in synthesis of - Answer-rRNA
/.The first of Koch's postulates is - Answer-the microorganism must be present in every
case of the disease but absent from healthy organisms.
/.Which of the following bacterial structures are most directly linked to swimming motility
in chemotaxis? - Answer-flagella
/.True or False: Gram-positive bacteria have a thinner layer of peptidoglycan than gram-
negative bacteria - Answer-False
/.Cytosol - Answer-liquid component of the cytoplasm
/.What is useful in distinguishing between prokaryotic and eukaryotic cells? - Answer-
The presence of membrane-delimited organelles within the cytoplasm
/.ER - Answer-Location for protein synthesis and some protein modifications
/.which of the following advances best describes that today we maybe experiencing a
second golden age of microbiology? - Answer-genomic testing
/.Perisomes - Answer-Location for storage of protective compounds that break down
lipids and detoxify certain chemicals
/.function of mitochondria - Answer-tricarboxylic acid cycle enzyme reaction, electron
transport, ATP synthesis
/.Lacks linear chromosome and has a peptidoglycan cell wall - Answer-Bacteria
/.Which of the following describes an organism that rarely contains plasmid? - Answer-
Eukarya
/.Which acellullar agent/s are composed of nucleic acids only? Check all that apply. -
Answer-satellite, prion, virus
, /.Which of the following describes an organism that contains 80S ribosomes? - Answer-
eukarya
/.An external structure unique to archaea are the ______. - Answer-cannulae
/.If you found a microbe with an S-layer as the major component of its cell wall, how
would you identify this microbe? - Answer-Archaeon
/.Cell wall of bacteria is composed of - Answer-Cellulose and peptidoglycan
/.Lysosomes - Answer-Location for storage of many types of degradative digestive
enzymes. Arise from the Golgi apparatus. Contains hydrolytic enzymes.
/.The plasma membrane of eukaryotes contains all of the following EXCEPT - Answer-
peptidoglycan.
/.Which of the following structures are external to both Bacterial and Archaeal cells? -
Answer-pilli
/.Bacillus - Answer-rod-shaped
/.If you remove the peptidoglycan layer from a Gram-positive cell, it would still stain
purple with a Gram stain. - Answer-false
/.External structures that are found in Archaea but not Bacteria are _________. -
Answer-hami
/.periplasmic space - Answer-only found in gram-positive bacteria
/.Which of the following is NOT a function of the capsule? - Answer-used for regulating
nutrient uptake
/.All of the following statements about the periplasmic space are true except: - Answer-It
is found only in gram-positive bacteria.
/.Flexible bacteria with a helical shape are called - Answer-spirochetes.
/.Which of the following lacks linear chromosomes and peptidoglycan cell walls? -
Answer-Archaea
/.Who of the following provided evidence that contributed to discredit the concept of
spontaneous generation? - Answer-Pasteur
/.Lysosomes are membrane-bound vesicles that arise from the __________. - Answer-
golgi apparatus
