BISC 101 Lab exam UPDATED ACTUAL
Questions and CORRECT Answers
Serological Pipette - CORRECT ANSWER✔✔- Used to accurately dispense volumes of
liquids
pasteur pipette - CORRECT ANSWER✔✔- Used to dispense liquids when accuracy is less
important , and to gently mix liquid mixtures by pipetting in and out
Preparing wet mounts on glass slides - CORRECT ANSWER✔✔- To spread a sample thin
enough ( between a glass slide and a glass cover slip) to view clearly under a microscope
A simple chemical test : Using Iodine to test for starch - CORRECT ANSWER✔✔- To
determine whether a sample contains starch
Starch= plant/veggies
No starch = animals
Cheesecloth and funnel filter instructions - CORRECT ANSWER✔✔- Used to filter out
larger solids while allowing liquids and smaller particles ( eg. Cells, organelles, etc) to pass
through
how do molecules pass the membrane - CORRECT ANSWER✔✔- active/facilitated
transport, selective permeability of lipid bilayer, and concentration gradient
compound microscope - CORRECT ANSWER✔✔- Used to view objects at 40x 100x or
400x magnification - to see cells
spectrophotometer - CORRECT ANSWER✔✔- Used to measure how much light ( of each
specific wavelength/colour) is absorbed by a sample. A prism is used to split the light, so that
you can test one wavelength at a time across a wider range of possible wavelengths
eppendorf/IEC centrifuge - CORRECT ANSWER✔✔- Used to separate the particles within a
mixture into layers according to their relative densities, by spinning the mixture at extremely
high speeds. The densest particles are found at the bottom of the table
,Independent variable - CORRECT ANSWER✔✔- The variable that is manipulated and its
effect on the dependent variable is measured
Hypertonic - CORRECT ANSWER✔✔- When the concentration of solute is greater outside
than inside, water flows out= causes cell to shrivel and shrink in size
Hypotonic - CORRECT ANSWER✔✔- When the concentration of solute is greater inside the
cell than outside, water flows in= causes cell to swell and burst
isotonic - CORRECT ANSWER✔✔- when the concentration of both the solute and solvent is
equal so theres no net movement
What happens when you put a cell into a hypotonic solution? - CORRECT ANSWER✔✔-
fills to almost bursting
what happens when you put a cell into a hypertonic solution - CORRECT ANSWER✔✔-
shrivels
what happens when you put a cell into an isotonic solution - CORRECT ANSWER✔✔-
normal
the cell membrane is permeable to - CORRECT ANSWER✔✔- Small hydrophobic
molecules ( ex. O2, CO2 , N2 , Benzene)
Small uncharged molecules ( ex. H2O, glycerol)
the cell membrane is not permeable to - CORRECT ANSWER✔✔- Large, uncharged
molecules (ex. Glucose, sucrose)
Ions (ex. Cl-, K + , Na+)
why does Increasing the temperature increases the membrane permeability - CORRECT
ANSWER✔✔- o When raising the temperature, its fluidity increases, the atoms move faster
including those that make up the phospholipid bilayers .
, o This causes the membrane to be more fluid since the phospholipids are also moving and
that it can be easily penetrated by other molecules
Why do you think a solute's solubility affects its ability to cross the RBC membrane ? -
CORRECT ANSWER✔✔- o A solutes solubility in oil affect its ability to cross the RBC
membrane helps determine its polarity. Since Water is polar and oil is non polar , it means
that the molecules are not attracted to each other. Thus, the more soluble in oil it is, the more
polar the solute is.
What color are the P atoms in 3D molecule of DNA? what is phosphorus bonded to, to make
a phosphate group? - CORRECT ANSWER✔✔- - Purple
attached to the 5 carbon sugar at the 5th carbon (opposite to nitrogenous base /react-text
- it is made up of a P with 4O bonded to it to make a phosphorus group
What is the colour of the nitrogen atoms, - CORRECT ANSWER✔✔- -Blue
-N is bonded to the first carbon of the 5 carbon molecule
what bond is in purine and pyrimidines - CORRECT ANSWER✔✔- -A bonded to T by 2
hydrogen bonds (double bond)
-G bonded to C by 3 hydrogen bonds (triple bond)
what is N bonded to from nitrogenous base? - CORRECT ANSWER✔✔- -It forms either
A,T,C, or G base
-(A,G) = purine base (two rings)
-(T,C) = pyrimidine base (one ring)
What is the colour of carbon atoms ? how do carbon atoms bond together to make the 5
carbon sugar? - CORRECT ANSWER✔✔- black, bonded to hydrogen atoms forming 5
carbon sugar
what is the colour of the hydrogen atoms and size - CORRECT ANSWER✔✔- white and
small
Questions and CORRECT Answers
Serological Pipette - CORRECT ANSWER✔✔- Used to accurately dispense volumes of
liquids
pasteur pipette - CORRECT ANSWER✔✔- Used to dispense liquids when accuracy is less
important , and to gently mix liquid mixtures by pipetting in and out
Preparing wet mounts on glass slides - CORRECT ANSWER✔✔- To spread a sample thin
enough ( between a glass slide and a glass cover slip) to view clearly under a microscope
A simple chemical test : Using Iodine to test for starch - CORRECT ANSWER✔✔- To
determine whether a sample contains starch
Starch= plant/veggies
No starch = animals
Cheesecloth and funnel filter instructions - CORRECT ANSWER✔✔- Used to filter out
larger solids while allowing liquids and smaller particles ( eg. Cells, organelles, etc) to pass
through
how do molecules pass the membrane - CORRECT ANSWER✔✔- active/facilitated
transport, selective permeability of lipid bilayer, and concentration gradient
compound microscope - CORRECT ANSWER✔✔- Used to view objects at 40x 100x or
400x magnification - to see cells
spectrophotometer - CORRECT ANSWER✔✔- Used to measure how much light ( of each
specific wavelength/colour) is absorbed by a sample. A prism is used to split the light, so that
you can test one wavelength at a time across a wider range of possible wavelengths
eppendorf/IEC centrifuge - CORRECT ANSWER✔✔- Used to separate the particles within a
mixture into layers according to their relative densities, by spinning the mixture at extremely
high speeds. The densest particles are found at the bottom of the table
,Independent variable - CORRECT ANSWER✔✔- The variable that is manipulated and its
effect on the dependent variable is measured
Hypertonic - CORRECT ANSWER✔✔- When the concentration of solute is greater outside
than inside, water flows out= causes cell to shrivel and shrink in size
Hypotonic - CORRECT ANSWER✔✔- When the concentration of solute is greater inside the
cell than outside, water flows in= causes cell to swell and burst
isotonic - CORRECT ANSWER✔✔- when the concentration of both the solute and solvent is
equal so theres no net movement
What happens when you put a cell into a hypotonic solution? - CORRECT ANSWER✔✔-
fills to almost bursting
what happens when you put a cell into a hypertonic solution - CORRECT ANSWER✔✔-
shrivels
what happens when you put a cell into an isotonic solution - CORRECT ANSWER✔✔-
normal
the cell membrane is permeable to - CORRECT ANSWER✔✔- Small hydrophobic
molecules ( ex. O2, CO2 , N2 , Benzene)
Small uncharged molecules ( ex. H2O, glycerol)
the cell membrane is not permeable to - CORRECT ANSWER✔✔- Large, uncharged
molecules (ex. Glucose, sucrose)
Ions (ex. Cl-, K + , Na+)
why does Increasing the temperature increases the membrane permeability - CORRECT
ANSWER✔✔- o When raising the temperature, its fluidity increases, the atoms move faster
including those that make up the phospholipid bilayers .
, o This causes the membrane to be more fluid since the phospholipids are also moving and
that it can be easily penetrated by other molecules
Why do you think a solute's solubility affects its ability to cross the RBC membrane ? -
CORRECT ANSWER✔✔- o A solutes solubility in oil affect its ability to cross the RBC
membrane helps determine its polarity. Since Water is polar and oil is non polar , it means
that the molecules are not attracted to each other. Thus, the more soluble in oil it is, the more
polar the solute is.
What color are the P atoms in 3D molecule of DNA? what is phosphorus bonded to, to make
a phosphate group? - CORRECT ANSWER✔✔- - Purple
attached to the 5 carbon sugar at the 5th carbon (opposite to nitrogenous base /react-text
- it is made up of a P with 4O bonded to it to make a phosphorus group
What is the colour of the nitrogen atoms, - CORRECT ANSWER✔✔- -Blue
-N is bonded to the first carbon of the 5 carbon molecule
what bond is in purine and pyrimidines - CORRECT ANSWER✔✔- -A bonded to T by 2
hydrogen bonds (double bond)
-G bonded to C by 3 hydrogen bonds (triple bond)
what is N bonded to from nitrogenous base? - CORRECT ANSWER✔✔- -It forms either
A,T,C, or G base
-(A,G) = purine base (two rings)
-(T,C) = pyrimidine base (one ring)
What is the colour of carbon atoms ? how do carbon atoms bond together to make the 5
carbon sugar? - CORRECT ANSWER✔✔- black, bonded to hydrogen atoms forming 5
carbon sugar
what is the colour of the hydrogen atoms and size - CORRECT ANSWER✔✔- white and
small
