MCB 2050 Quiz Bank with
complete verified solutions
What is meant by the term "affinity system" as used in the reading?
A system which contains a relatively large dense particle (ex. agarose or
sepharose bead) which allows bound objects to be removed from solution
via centrifugation.
A reporter plasmid is which GFP is fused to the open reading frame of a
gene of interest (protein X) is used to:
visualize the subcellular localization of the protein of interest
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What is the function of a Multiple Cloning Site (MCS) in a plasmid?
This sequence is optimized for the insertion of DNA fragments.
Which of the following is a difference between the use of a reporter gene
to study gene regulation (ex. promoter deletion mapping with a reporter
protein) and using a reporter gene to study protein localization? Select all
that apply?
Only gene expression studies utilize engineered plasmids which contain
the reporter gene.
, The pull down assay described in Tutorial 7 is similar to co-
immunoprecipitation technique described earlier in this course. Which of
the following are differences between the IP/co-IP techniques and the pull
down assay? Select all that apply.
The co-IP assay utilizes antibody specific to the target protein.
AND
You are creating a tagged protein fragment for use in a pull down assay as
depicted in Figure 2 of the reading in order to isolate importin - α. What
sequence should your fragment contain?
an NLS sequence
Which of the following is NOT a conserved eukaryotic promoter element ?
GCTA box
A/an i)________ element is a regulatory DNA sequence that binds
transcription factors to increase transcriptional activation in a gene
specific manner. These sequences can be found ii)__________ of the
transcription start site.
i) enhancer; ii) upstream or downstream
Which of the following levels of gene regulation is most frequently used in
eukaryotic regulation?
transcriptional regulation
Proteins that bind specific gene regulatory DNA sequences to inhibit gene
expression are called:
Repressors
In order to use an electrophoretic (gel) mobility shift assay to confirm that
the initiation factor TFIID binds the TATA box of the eukaryotic promoter
which of the following reagents would be required? Select all that apply.
labeled probe containing the sequence TATAAA
anti-TFIID antibody
In SDS-polyacrylamide gel electrophoresis, proteins are resolved based on
their:
mass
What is one thing that distinguishes SDS-PAGE from basic gel
electrophoresis (GE)?
In SDS_PAGE an electric field is applied to the running gel.
complete verified solutions
What is meant by the term "affinity system" as used in the reading?
A system which contains a relatively large dense particle (ex. agarose or
sepharose bead) which allows bound objects to be removed from solution
via centrifugation.
A reporter plasmid is which GFP is fused to the open reading frame of a
gene of interest (protein X) is used to:
visualize the subcellular localization of the protein of interest
Previous
Play
Next
Rewind 10 seconds
Move forward 10 seconds
Unmute
0:00
/
0:15
Full screen
Brainpower
Read More
What is the function of a Multiple Cloning Site (MCS) in a plasmid?
This sequence is optimized for the insertion of DNA fragments.
Which of the following is a difference between the use of a reporter gene
to study gene regulation (ex. promoter deletion mapping with a reporter
protein) and using a reporter gene to study protein localization? Select all
that apply?
Only gene expression studies utilize engineered plasmids which contain
the reporter gene.
, The pull down assay described in Tutorial 7 is similar to co-
immunoprecipitation technique described earlier in this course. Which of
the following are differences between the IP/co-IP techniques and the pull
down assay? Select all that apply.
The co-IP assay utilizes antibody specific to the target protein.
AND
You are creating a tagged protein fragment for use in a pull down assay as
depicted in Figure 2 of the reading in order to isolate importin - α. What
sequence should your fragment contain?
an NLS sequence
Which of the following is NOT a conserved eukaryotic promoter element ?
GCTA box
A/an i)________ element is a regulatory DNA sequence that binds
transcription factors to increase transcriptional activation in a gene
specific manner. These sequences can be found ii)__________ of the
transcription start site.
i) enhancer; ii) upstream or downstream
Which of the following levels of gene regulation is most frequently used in
eukaryotic regulation?
transcriptional regulation
Proteins that bind specific gene regulatory DNA sequences to inhibit gene
expression are called:
Repressors
In order to use an electrophoretic (gel) mobility shift assay to confirm that
the initiation factor TFIID binds the TATA box of the eukaryotic promoter
which of the following reagents would be required? Select all that apply.
labeled probe containing the sequence TATAAA
anti-TFIID antibody
In SDS-polyacrylamide gel electrophoresis, proteins are resolved based on
their:
mass
What is one thing that distinguishes SDS-PAGE from basic gel
electrophoresis (GE)?
In SDS_PAGE an electric field is applied to the running gel.
