MB (ASCP) PRACTICE EXAM 2024-2025 QUESTIONS
AND ANSWERS.
What are sources of DNA damage? - <<<Answers>>>1.)
Chemical damage
2.) Attack by water
3.) Radiation damage
Mutation in UGT1A1 affects metabolism of what drug? -
<<<Answers>>>Sorafenib
List 3 examples of trinucleotide repeat disorders. -
<<<Answers>>>1.) Spinocerebellar Ataxia Type 8
2.) Huntington Disease
3.) Fragile X
This polymerase is involved in "initiation of DNA replication
and has primase activity"? - <<<Answers>>>Pol alpha
Gene mutated in Ataxia Telangiectasia -
<<<Answers>>>ATM
Inherited mutations in this gene cause Li-Fraumeni syndrome
- <<<Answers>>>TP53
Gene on chromosome 13 that is involved in translocations in
rhabdomyosarcoma - <<<Answers>>>FKHR (FOXO1A)
80% of Synovial sarcoma are caused by this translocation -
<<<Answers>>>t(X;18)
, MB (ASCP) PRACTICE EXAM 2024-2025 QUESTIONS
AND ANSWERS.
Most common translocation in 85% of Ewing's Sarcoma -
<<<Answers>>>t(11,22)(q24,q12)
Most common oncogene in human cancer -
<<<Answers>>>KRAS
CML (chronic myelogenous leukemia) is caused by this
translocation - <<<Answers>>>t(9;22)
Fusion gene created by the translocation that causes CML -
<<<Answers>>>BCR-ABL
Which of the following will more likely lower stringency
conditions in the washing step of a hybridization experiment?
- <<<Answers>>>Increase the concentration of salt in the
wash solution buffer
The ideal spacing between the -10 and -35 regions of the
prokaryotice promoter is: - <<<Answers>>>17
Purines have __ ring(s) and pyrimidines have __ ring(s) -
<<<Answers>>>Purines = 2, pyrimidines = 1
PCR method that works by generating a signal at the
annealing step (i.e. when the probe binds its target) of the
PCR reaction: - <<<Answers>>>molecular beacons
, MB (ASCP) PRACTICE EXAM 2024-2025 QUESTIONS
AND ANSWERS.
After a patient received a bone marrow transplant, what virus
is the patient more susceptible to? - <<<Answers>>>CMV
(Cytomegalovirus)
Which of the subunits of the RNA polymerase holoenzyme is
responsible for promoter recognition? - <<<Answers>>>Beta
subunit
In the field of molecular diagnostics, which one of the
following genes is responsible for the synthesis of DNA,
promote cell division, and inhibit cell death? -
<<<Answers>>>proto-oncogenes
What assay amplifies the target using DNA polymerase? -
<<<Answers>>>PCR
What genes would be screened in a lung cancer panel? -
<<<Answers>>>KRAS, EGFR, and ALK
What is a vector? - <<<Answers>>>helps carry DNA into the
cell, i.e. plasmids or viruses
Which PCR-based methologies employs a set of primer pairs
designed in such a way that they overlap, but only one will
bind to the target in the presence of SNPs? -
AND ANSWERS.
What are sources of DNA damage? - <<<Answers>>>1.)
Chemical damage
2.) Attack by water
3.) Radiation damage
Mutation in UGT1A1 affects metabolism of what drug? -
<<<Answers>>>Sorafenib
List 3 examples of trinucleotide repeat disorders. -
<<<Answers>>>1.) Spinocerebellar Ataxia Type 8
2.) Huntington Disease
3.) Fragile X
This polymerase is involved in "initiation of DNA replication
and has primase activity"? - <<<Answers>>>Pol alpha
Gene mutated in Ataxia Telangiectasia -
<<<Answers>>>ATM
Inherited mutations in this gene cause Li-Fraumeni syndrome
- <<<Answers>>>TP53
Gene on chromosome 13 that is involved in translocations in
rhabdomyosarcoma - <<<Answers>>>FKHR (FOXO1A)
80% of Synovial sarcoma are caused by this translocation -
<<<Answers>>>t(X;18)
, MB (ASCP) PRACTICE EXAM 2024-2025 QUESTIONS
AND ANSWERS.
Most common translocation in 85% of Ewing's Sarcoma -
<<<Answers>>>t(11,22)(q24,q12)
Most common oncogene in human cancer -
<<<Answers>>>KRAS
CML (chronic myelogenous leukemia) is caused by this
translocation - <<<Answers>>>t(9;22)
Fusion gene created by the translocation that causes CML -
<<<Answers>>>BCR-ABL
Which of the following will more likely lower stringency
conditions in the washing step of a hybridization experiment?
- <<<Answers>>>Increase the concentration of salt in the
wash solution buffer
The ideal spacing between the -10 and -35 regions of the
prokaryotice promoter is: - <<<Answers>>>17
Purines have __ ring(s) and pyrimidines have __ ring(s) -
<<<Answers>>>Purines = 2, pyrimidines = 1
PCR method that works by generating a signal at the
annealing step (i.e. when the probe binds its target) of the
PCR reaction: - <<<Answers>>>molecular beacons
, MB (ASCP) PRACTICE EXAM 2024-2025 QUESTIONS
AND ANSWERS.
After a patient received a bone marrow transplant, what virus
is the patient more susceptible to? - <<<Answers>>>CMV
(Cytomegalovirus)
Which of the subunits of the RNA polymerase holoenzyme is
responsible for promoter recognition? - <<<Answers>>>Beta
subunit
In the field of molecular diagnostics, which one of the
following genes is responsible for the synthesis of DNA,
promote cell division, and inhibit cell death? -
<<<Answers>>>proto-oncogenes
What assay amplifies the target using DNA polymerase? -
<<<Answers>>>PCR
What genes would be screened in a lung cancer panel? -
<<<Answers>>>KRAS, EGFR, and ALK
What is a vector? - <<<Answers>>>helps carry DNA into the
cell, i.e. plasmids or viruses
Which PCR-based methologies employs a set of primer pairs
designed in such a way that they overlap, but only one will
bind to the target in the presence of SNPs? -
