MEDICAL BIOLOGY AND GENETICS
c c c
for
PHARMACOLOGISTS
c
Week11
DNA Replication
c c
Dr. Ceyhan Ceran Serdar – 2021
c c c c c
,Reference Books
c
, Links to useful animations
c c c
https://www.youtube.com/watch?v=mxAjSBhDkBQ
https://www.youtube.com/watch?v=TNKWgcFPHqw
https://www.youtube.com/watch?v=0Ha9nppnwOc&t=187s
https://www.youtube.com/watch?v=EYGrElVyHnU
https://youtu.be/mDZLiZB0iPY
https://youtu.be/X_tYrnv_o6A
https://www.youtube.com/watch?v=efstlgoynlk
https://www.youtube.com/watch?v=kp0esidDr-c
https://www.youtube.com/watch?v=vP8-5Bhd2ag&t=266s
https://www.youtube.com/watch?v=FMcYKec-Xx4&t=2s
, Learning Objectives c
Students who successfully completes this course;
c c c c c
1. Explain the semi-conservative mode of DNA-replication.
c c c c c
2. Compare the mutation rates of human genome and E. coli genome per generation.
c c c c c c c c c c c c
3. Explain how cells keep the mutation rate low enough to ensure accurate transmission of genetic information
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camong generations, despite high mutation rate of DNA replication.
c c c c c c c c
4. Describe origin of replication, replication forks, replication bubbles and replicons.
c c c c c c c c c
5. Explain the significance of origins of replications being AT-rich
c c c c c c c c
6. Explain DNA replication, describing the roles of DNA topoisomerase, DNA polymerase, DNA helicase,
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c SSBP, Primase, DNA ligase.
c c c
7. Explain the significance of initiating DNA replication by RNA primers and explain how our chromosomes do
c c c c c c c c c c c c c c c
c not contain RNA, although RNA primers are used in the DNA replication process.
c c c c c c c c c c c c
8. Explain the bidirectional mode of DNA replication, by describing Okazaki fragments and by explaining
c c c c c c c c c c c c c
c the events taking place on each strand during DNA replication.
c c c c c c c c c
9. Compare endonucleases and exonucleases, explaining their functions, and explain how they function
c c c c c c c c c c c
c in resolving DNA supercoils during DNA replication.
c c c c c c
10. Explain how DNA molecules are duplicated only once during each DNA replication.
c c c c c c c c c c c
11. Explain how epigenetic marks are preserved during each DNA replication.
c c c c c c c c c
12. Describe telomere, telomerase and explain how they serve a eukaryotic cell.
c c c c c c c c c c
13. Describe the interaction between telomeres and aging and discuss whether telomerase-based strategies can
c c c c c c c c c c c c
csafely be used to prevent aging.
c c c c c
14. Explain how spontaneous mutations occur during DNA replication
c c c c c c c
15. List the endogenous and exogenous factors that cause DNA damage
c c c c c c c c c
c c c
for
PHARMACOLOGISTS
c
Week11
DNA Replication
c c
Dr. Ceyhan Ceran Serdar – 2021
c c c c c
,Reference Books
c
, Links to useful animations
c c c
https://www.youtube.com/watch?v=mxAjSBhDkBQ
https://www.youtube.com/watch?v=TNKWgcFPHqw
https://www.youtube.com/watch?v=0Ha9nppnwOc&t=187s
https://www.youtube.com/watch?v=EYGrElVyHnU
https://youtu.be/mDZLiZB0iPY
https://youtu.be/X_tYrnv_o6A
https://www.youtube.com/watch?v=efstlgoynlk
https://www.youtube.com/watch?v=kp0esidDr-c
https://www.youtube.com/watch?v=vP8-5Bhd2ag&t=266s
https://www.youtube.com/watch?v=FMcYKec-Xx4&t=2s
, Learning Objectives c
Students who successfully completes this course;
c c c c c
1. Explain the semi-conservative mode of DNA-replication.
c c c c c
2. Compare the mutation rates of human genome and E. coli genome per generation.
c c c c c c c c c c c c
3. Explain how cells keep the mutation rate low enough to ensure accurate transmission of genetic information
c c c c c c c c c c c c c c c
camong generations, despite high mutation rate of DNA replication.
c c c c c c c c
4. Describe origin of replication, replication forks, replication bubbles and replicons.
c c c c c c c c c
5. Explain the significance of origins of replications being AT-rich
c c c c c c c c
6. Explain DNA replication, describing the roles of DNA topoisomerase, DNA polymerase, DNA helicase,
c c c c c c c c c c c c
c SSBP, Primase, DNA ligase.
c c c
7. Explain the significance of initiating DNA replication by RNA primers and explain how our chromosomes do
c c c c c c c c c c c c c c c
c not contain RNA, although RNA primers are used in the DNA replication process.
c c c c c c c c c c c c
8. Explain the bidirectional mode of DNA replication, by describing Okazaki fragments and by explaining
c c c c c c c c c c c c c
c the events taking place on each strand during DNA replication.
c c c c c c c c c
9. Compare endonucleases and exonucleases, explaining their functions, and explain how they function
c c c c c c c c c c c
c in resolving DNA supercoils during DNA replication.
c c c c c c
10. Explain how DNA molecules are duplicated only once during each DNA replication.
c c c c c c c c c c c
11. Explain how epigenetic marks are preserved during each DNA replication.
c c c c c c c c c
12. Describe telomere, telomerase and explain how they serve a eukaryotic cell.
c c c c c c c c c c
13. Describe the interaction between telomeres and aging and discuss whether telomerase-based strategies can
c c c c c c c c c c c c
csafely be used to prevent aging.
c c c c c
14. Explain how spontaneous mutations occur during DNA replication
c c c c c c c
15. List the endogenous and exogenous factors that cause DNA damage
c c c c c c c c c
