MIP 300 Final
Questions and Answers
Which of the following are eukaryotic microbes?✅✅A) Viruses
B) Protozoa
C) Bacteria
D) Yeasts
E) B and D (CORRECT)
(True/False)
Protozoa are considered "transitional" organisms because they may not contaon all the usual
membrane-bound organelles you would expect to find in a eukaryotic cell✅✅True
You just landed a position as a Graduate Teaching Assistant in a microbiology laboratory class
and are grading laboratory notebooks. How would you grade this student's lab book entry?
Streptococus Pyogenes✅✅Incorrect, because the species name should be lower case and
because the student forgot to underline or italicize genus and species names
What kingdom are viruses classified in?✅✅Monera, Virilae, Fungi, Anamalia
None of the above (CORRECT)- viruses are not classified in either the kingdom or the domain
systems
You have the opportunity to purchase a new microscope. Which of the scopes listed below, all of
which cost $2,000, will you get the most powerful microscope for you money?✅✅Resolving
power= 1mm
Resolving power= 4um
Resolving power= 1um
Resolving power=0.4um (CORRECT)- this microscope will allow you to see the smallest
specimen, so it has the best resolving power
Resolvingpower=0.4mm
You perform an acid-fast stain on Mycobacterium tuberculosis are realize at the end that you
forgot to use heat/ What color will the cells be?✅✅Colorless- heat is needed to drive the pink
stain(or any stain) past the mycolic acid layer. Without heat, the primary pink stain will not
penetrate and the cell will remain clear
You discover a new bacterium. Its cell envelope shows high concentrations of
phosphatidylethanolmine and another type of phospholipid that is associated with long
polysaccharide chains of unusual sugars. This organism is likely?✅✅Gram-negative
,Species can be broken down even further. What are different groupings within species called in
dogs?✅✅Breeds
Species can be broken down even further. What are different groupings within species called in
cats?✅✅Breeds
Bacterial axial filament stains will stain the cell✅✅
Bacterial flagella stains will stain the cell✅✅external to the cell wall
In eukaryotic cells, the function of ribosomes is✅✅protein production
In bacteria, the function of ribosomes is✅✅protein production
Which of the following types of microscope will allow you to visualize FITC- labeled stained
viruses that are infecting a cell✅✅Fluorescent
Which of the following types of microscope will allow you to most readily visualize live bacteria
in a water sample without staining?✅✅Phase Contrast
Which of the following types of microscope will allow you to visualize viruses inside a
cell?✅✅Electron
Species can be broken down even further. What are different groupings within species called in
bacteria?✅✅Strains
Species can be broken down even further. What are different groupings within species called in
fungi?✅✅Strains
(True/False)
Viruses will have ribosomes✅✅False
Species can be broken down even further. What are different groupings within species called in
humans?✅✅Races
A differential stain✅✅1) will stain some bacteria differently than other
2) is used to determine differences between cell structures
3) is used to give color to cells so they can easily be observed under a microscope
4) is used to selectively grow certain types of bacteria
5) 1 and 2 (CORRECT)
In an endospore stain, the positive cells will have which of the following
appearances?✅✅Green spots within a red cell
, Species can be broken down even further. What are different groupings within species called in
viruses?✅✅Strains
What components of the peptidoglycan can be altered to change the thickness of a Gram-
Negative peptidoglycan layer?✅✅The number of NAG and NAM sheets (Gram0Negative
bacteria don't have interbridges, only direct covalent linkage between tetrapeptides, thus the
number of NAG-NAM sheets is the only factor contributing to varied thickness of the Gram-
Ngative ppg)
What components of the peptidoglycan can be altered to change the thickness of a Gram-Positive
peptidoglycan layer?✅✅A) the number of NAG and NAM sheets
B) the type of amino acids in the interbridge
C) the number of amino acids in the interbridge
D) A and B
E) A, B and C (CORRCT)
Gram-Positive can have different numbers of NAG-NAM sheets, and there can be different types
and different numbers of amino acids within the interbridges, all of which alter the thickness of
the ppg)
You do a Gram stain. You realize when you finish that you forgot to add Gram's Iodine. What
color will a Gram-Positive cell be at the end of staining?✅✅Pink
Crystal violet is NOT retained within the thick ppg(since it isn't aggregated with the mordant)
upon the wash step, thus it will stain pink from the secondary stain
You isolate a cell and find that it has 70S ribosomes. This cell could be✅✅Prokaryote
Eukaryotes
Either Prokaryote or Eukaryote (CORREECT)
Because both cell types have 70S ribosomes. The prokaryote have only 70S ribosomes.
Eukaryotes, on the other hand, have 80S ribosomes that callry out the majority of protein
synthesis for the cell, but they also have 70S ribosomes in their mitochondria and
chloroplasts(endosymbiont theory)
(True/False)
Plasmids are not required for bacterial cell growth or reproduction✅✅True
plasmids are not requires by the cell, but they do confer upon the cell selective advantage
(True/False)
Most spore-forming bacteria are Gram-Positive✅✅True
Questions and Answers
Which of the following are eukaryotic microbes?✅✅A) Viruses
B) Protozoa
C) Bacteria
D) Yeasts
E) B and D (CORRECT)
(True/False)
Protozoa are considered "transitional" organisms because they may not contaon all the usual
membrane-bound organelles you would expect to find in a eukaryotic cell✅✅True
You just landed a position as a Graduate Teaching Assistant in a microbiology laboratory class
and are grading laboratory notebooks. How would you grade this student's lab book entry?
Streptococus Pyogenes✅✅Incorrect, because the species name should be lower case and
because the student forgot to underline or italicize genus and species names
What kingdom are viruses classified in?✅✅Monera, Virilae, Fungi, Anamalia
None of the above (CORRECT)- viruses are not classified in either the kingdom or the domain
systems
You have the opportunity to purchase a new microscope. Which of the scopes listed below, all of
which cost $2,000, will you get the most powerful microscope for you money?✅✅Resolving
power= 1mm
Resolving power= 4um
Resolving power= 1um
Resolving power=0.4um (CORRECT)- this microscope will allow you to see the smallest
specimen, so it has the best resolving power
Resolvingpower=0.4mm
You perform an acid-fast stain on Mycobacterium tuberculosis are realize at the end that you
forgot to use heat/ What color will the cells be?✅✅Colorless- heat is needed to drive the pink
stain(or any stain) past the mycolic acid layer. Without heat, the primary pink stain will not
penetrate and the cell will remain clear
You discover a new bacterium. Its cell envelope shows high concentrations of
phosphatidylethanolmine and another type of phospholipid that is associated with long
polysaccharide chains of unusual sugars. This organism is likely?✅✅Gram-negative
,Species can be broken down even further. What are different groupings within species called in
dogs?✅✅Breeds
Species can be broken down even further. What are different groupings within species called in
cats?✅✅Breeds
Bacterial axial filament stains will stain the cell✅✅
Bacterial flagella stains will stain the cell✅✅external to the cell wall
In eukaryotic cells, the function of ribosomes is✅✅protein production
In bacteria, the function of ribosomes is✅✅protein production
Which of the following types of microscope will allow you to visualize FITC- labeled stained
viruses that are infecting a cell✅✅Fluorescent
Which of the following types of microscope will allow you to most readily visualize live bacteria
in a water sample without staining?✅✅Phase Contrast
Which of the following types of microscope will allow you to visualize viruses inside a
cell?✅✅Electron
Species can be broken down even further. What are different groupings within species called in
bacteria?✅✅Strains
Species can be broken down even further. What are different groupings within species called in
fungi?✅✅Strains
(True/False)
Viruses will have ribosomes✅✅False
Species can be broken down even further. What are different groupings within species called in
humans?✅✅Races
A differential stain✅✅1) will stain some bacteria differently than other
2) is used to determine differences between cell structures
3) is used to give color to cells so they can easily be observed under a microscope
4) is used to selectively grow certain types of bacteria
5) 1 and 2 (CORRECT)
In an endospore stain, the positive cells will have which of the following
appearances?✅✅Green spots within a red cell
, Species can be broken down even further. What are different groupings within species called in
viruses?✅✅Strains
What components of the peptidoglycan can be altered to change the thickness of a Gram-
Negative peptidoglycan layer?✅✅The number of NAG and NAM sheets (Gram0Negative
bacteria don't have interbridges, only direct covalent linkage between tetrapeptides, thus the
number of NAG-NAM sheets is the only factor contributing to varied thickness of the Gram-
Ngative ppg)
What components of the peptidoglycan can be altered to change the thickness of a Gram-Positive
peptidoglycan layer?✅✅A) the number of NAG and NAM sheets
B) the type of amino acids in the interbridge
C) the number of amino acids in the interbridge
D) A and B
E) A, B and C (CORRCT)
Gram-Positive can have different numbers of NAG-NAM sheets, and there can be different types
and different numbers of amino acids within the interbridges, all of which alter the thickness of
the ppg)
You do a Gram stain. You realize when you finish that you forgot to add Gram's Iodine. What
color will a Gram-Positive cell be at the end of staining?✅✅Pink
Crystal violet is NOT retained within the thick ppg(since it isn't aggregated with the mordant)
upon the wash step, thus it will stain pink from the secondary stain
You isolate a cell and find that it has 70S ribosomes. This cell could be✅✅Prokaryote
Eukaryotes
Either Prokaryote or Eukaryote (CORREECT)
Because both cell types have 70S ribosomes. The prokaryote have only 70S ribosomes.
Eukaryotes, on the other hand, have 80S ribosomes that callry out the majority of protein
synthesis for the cell, but they also have 70S ribosomes in their mitochondria and
chloroplasts(endosymbiont theory)
(True/False)
Plasmids are not required for bacterial cell growth or reproduction✅✅True
plasmids are not requires by the cell, but they do confer upon the cell selective advantage
(True/False)
Most spore-forming bacteria are Gram-Positive✅✅True
