HISTOTECHNOLOGY EXAM 1 QUESTIONS (CHAPTER 2) 2024 WITH ACCURATE ANSWERS

HISTOTECHNOLOGY EXAM 1
QUESTIONS (CHAPTER 2) 2024 WITH
ACCURATE ANSWERS

, HISTOTECHNOLOGY EXAM 1
QUESTIONS (CHAPTER 2) 2024 WITH
ACCURATE ANSWERS
What temperature is the tissue frozen to in the cryostat? - CORRECT ANSWERS-20 to - 30 degrees
Celsius



Why is methanol added to ethanol? - CORRECT ANSWERSSo you can't drink it. poisonous.



If a pathologists wants to see the fat in tissue, how must the tissue be processed? - CORRECT
ANSWERSfrozen section and hand processed



At how many cassettes does the processor need to be changed for small biopsies? - CORRECT
ANSWERS400



At how many cassettes does the processor need to be changed for large tissue? - CORRECT
ANSWERS800-1000



What are the steps of processing? - CORRECT ANSWERSFixation, (decalcification), dehydration, clearing,
infiltration, embedding



The step of processing removes water for the next subsequent step to occur (xylene and paraffin) -
CORRECT ANSWERSdehydration



What accounts for the majority of tissue processing problems? **** - CORRECT ANSWERSincomplete
dehydration



How does one solve incomplete dehydration? - CORRECT ANSWERSReprocess in water and then
absolute alcohol. Or melt paraffin block, pat tissue with gauze, and soak in 3-5 changes of paraffin. Only
works with small amounts of water.