HISTOTECHNOLOGY I FINAL EXAM
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ANSWERS
,HISTOTECHNOLOGY I FINAL EXAM
PREP 2024 WITH 100% ACCURATE
ANSWERS
Tape coverslips - CORRECT ANSWERSused with robotic coverslip instruments; acetate film coated with
adhesive on one side, activated by xylene
Combined coverslip and mountant - CORRECT ANSWERSapplied to slide by pouring, dipping or spraying;
varnish-like substance that covers and protects section (does not require a glass coverslip)
Synthetic Resinous Mounting Medium - CORRECT ANSWERS1. dries quickly and when dried it isnt sticky
2. neutral pH
3. used with solvents (should match the clearing agent)
4. more volatile solvents produce more latent air bubbles
5. RI 1.5-1.55
What are the two common staining methods for nucleic acid in histology? - CORRECT ANSWERS1.
Feulgen Reaction
2. Methyl Green-Pyronin Method (DNA/RNA)
Feulgen Reaction tests for what? - CORRECT ANSWERSDNA
What does the Schiff reagent bind to in the Feulgen Reaction? - CORRECT ANSWERSAldehyde group
created by the hydrolysis of DNA
Why can't we use the Bouin fixative on tissues being tested with Feulgen? - CORRECT ANSWERSBouin
hydrolyzes tissues
In Feulgen reactions, what happens when the hydrolysis reactions are uncontrolled? - CORRECT
ANSWERSnegative results occur
, Methyl Green-Pyronin Y is used to demonstrate what? - CORRECT ANSWERSdifferentiates between DNA
and RNA, and identify plasma cells or immunoblasts
Where are high concentrations of immunoblasts and plasma cells found within the cell? - CORRECT
ANSWERSRibosomes
What does the Methyl Green-Pyronin Y bind selectively to? - CORRECT ANSWERSPolymerization in
DNA/RNA
What should you use as a control slide in the Methyl Green-Pyronin Y reaction? - CORRECT ANSWERSA
positive slide for plasma cells
Polychromatic stains result in what color results? - CORRECT ANSWERSMultiple colored tissue elements
Polychromatic dyes are - CORRECT ANSWERSsingle stain solutions that contain multiple dye molecules
What are two examples of polychromatic stains? - CORRECT ANSWERS1. Gomori 1-step Trichrome
2. Romanowsky stains
Can polychromatic stains stain orthochromatically, metachromatically or both? - CORRECT
ANSWERSBOTH
Romanowsky stains are a combination of what and what? - CORRECT ANSWERSEosin
Methylene Blue
Eosin is an acid or basic dye? - CORRECT ANSWERSAcid dye
Methylene Blue is an acid or basic dye? - CORRECT ANSWERSBasic dye
PREP 2024 WITH 100% ACCURATE
ANSWERS
,HISTOTECHNOLOGY I FINAL EXAM
PREP 2024 WITH 100% ACCURATE
ANSWERS
Tape coverslips - CORRECT ANSWERSused with robotic coverslip instruments; acetate film coated with
adhesive on one side, activated by xylene
Combined coverslip and mountant - CORRECT ANSWERSapplied to slide by pouring, dipping or spraying;
varnish-like substance that covers and protects section (does not require a glass coverslip)
Synthetic Resinous Mounting Medium - CORRECT ANSWERS1. dries quickly and when dried it isnt sticky
2. neutral pH
3. used with solvents (should match the clearing agent)
4. more volatile solvents produce more latent air bubbles
5. RI 1.5-1.55
What are the two common staining methods for nucleic acid in histology? - CORRECT ANSWERS1.
Feulgen Reaction
2. Methyl Green-Pyronin Method (DNA/RNA)
Feulgen Reaction tests for what? - CORRECT ANSWERSDNA
What does the Schiff reagent bind to in the Feulgen Reaction? - CORRECT ANSWERSAldehyde group
created by the hydrolysis of DNA
Why can't we use the Bouin fixative on tissues being tested with Feulgen? - CORRECT ANSWERSBouin
hydrolyzes tissues
In Feulgen reactions, what happens when the hydrolysis reactions are uncontrolled? - CORRECT
ANSWERSnegative results occur
, Methyl Green-Pyronin Y is used to demonstrate what? - CORRECT ANSWERSdifferentiates between DNA
and RNA, and identify plasma cells or immunoblasts
Where are high concentrations of immunoblasts and plasma cells found within the cell? - CORRECT
ANSWERSRibosomes
What does the Methyl Green-Pyronin Y bind selectively to? - CORRECT ANSWERSPolymerization in
DNA/RNA
What should you use as a control slide in the Methyl Green-Pyronin Y reaction? - CORRECT ANSWERSA
positive slide for plasma cells
Polychromatic stains result in what color results? - CORRECT ANSWERSMultiple colored tissue elements
Polychromatic dyes are - CORRECT ANSWERSsingle stain solutions that contain multiple dye molecules
What are two examples of polychromatic stains? - CORRECT ANSWERS1. Gomori 1-step Trichrome
2. Romanowsky stains
Can polychromatic stains stain orthochromatically, metachromatically or both? - CORRECT
ANSWERSBOTH
Romanowsky stains are a combination of what and what? - CORRECT ANSWERSEosin
Methylene Blue
Eosin is an acid or basic dye? - CORRECT ANSWERSAcid dye
Methylene Blue is an acid or basic dye? - CORRECT ANSWERSBasic dye
