BISC 261 Lecture 2 Test with Questions
with 100% Correct Answers| Latest Update
2024| Verified
What are fluorescence microscopes? - Answer * you're not hitting sample w/
white light, but a specific color or wavelength
* the light coming off the microscope will always be higher than the exit light
* black background & sample has color it gives off
**light will always be red shifted when it hits the sample**
Emission wavelength - Answer * color you hit the sample w/
Exitation wavelength - Answer * light that gets reflected off by the sample (lower in
energy)
Autofluorescene - Answer * organisms already have pigment
* 2 groups
- methanogens
- photosynthetic bacteria (cyanobacteria)
, What is a common type of dye for DNA? - Answer * DAPI
- can use it to see microbial growth; will let you know if your cells are growing
What is FISH? - Answer * fluorescence in situ hybridization
* a way of using fluorescent microscopy to see if bacteria are in an
environmental sample
What is electron microscopy? - Answer * how we know fine details of prokaryotic cells
* none light microscopy
- only form that doesn't use light
- instead manipulates electron waves
* instead of lenses, we use magnets to direct light
What are the 2 types of electron microscopy? - Answer * scanning: reveals surface
features of sample; can look at larger samples
* transmission: cuts through so you can see w/in
What is transmission electron microscopy? - Answer * requires thin cross section
* gives glimpses of cellular interiors
* requires heavy metal staining
* fixation process can introduce deformations
* electron pass through a sample
What is scanning electron microscopy? - Answer * can use larger
samples * electron beam interacts w/ surface of cell specimens
with 100% Correct Answers| Latest Update
2024| Verified
What are fluorescence microscopes? - Answer * you're not hitting sample w/
white light, but a specific color or wavelength
* the light coming off the microscope will always be higher than the exit light
* black background & sample has color it gives off
**light will always be red shifted when it hits the sample**
Emission wavelength - Answer * color you hit the sample w/
Exitation wavelength - Answer * light that gets reflected off by the sample (lower in
energy)
Autofluorescene - Answer * organisms already have pigment
* 2 groups
- methanogens
- photosynthetic bacteria (cyanobacteria)
, What is a common type of dye for DNA? - Answer * DAPI
- can use it to see microbial growth; will let you know if your cells are growing
What is FISH? - Answer * fluorescence in situ hybridization
* a way of using fluorescent microscopy to see if bacteria are in an
environmental sample
What is electron microscopy? - Answer * how we know fine details of prokaryotic cells
* none light microscopy
- only form that doesn't use light
- instead manipulates electron waves
* instead of lenses, we use magnets to direct light
What are the 2 types of electron microscopy? - Answer * scanning: reveals surface
features of sample; can look at larger samples
* transmission: cuts through so you can see w/in
What is transmission electron microscopy? - Answer * requires thin cross section
* gives glimpses of cellular interiors
* requires heavy metal staining
* fixation process can introduce deformations
* electron pass through a sample
What is scanning electron microscopy? - Answer * can use larger
samples * electron beam interacts w/ surface of cell specimens
