BISC 261 Lecture 3 Exam with Questions
with 100% Correct Answers| Latest Update
2024| Verified
What is DNA replication? - Answer * semi conservative
* The parent cell undergoes binary fission & there's 2 daughter cells
- Each daughter cell gets 1 strand from the parent & the other from DNA
replication
What does the origin of replication have? - Answer the DNAa sequence DNA
can bind to
Replication fork - Answer * where all the proteins for DNA replication are at
- As the replisomes moves down the DNA strand & unwinds it, the area where
nucleosomes are added is the replication fork
How many DNA poly III are at the replication fork? - Answer * multiple
What does the continuous (leading strand) have? - Answer * will have 1
DNA polymerase III
* moves 5' --> 3'
* starts with a phosphate
, What does the lagging strand have? - Answer * multiple DNA polymerase III
to synthesize the Okazaki fragments
* moves 3' --> 5'
* starts w/ a hydroxide
What is the daughter strand in relation to the parent strand? - Answer * opposite
What are the steps of Bacteria DNA replication proofreading? - Answer 1. DNA
polymerase makes an error & inserts the wrong nucleotide at the 3 prime end
of the strand being synthesized
2. The incorrect nucleotide separates from the template strand due to failure
to hydrogen bond properly. This causes the DNA POLY TO STOP
3. The 3 prime to 5 prime exonuclease activity of the DNA poly removes the
incorrect nucleotide
4. DNA poly restarts DNA synthesis, this time adding the correct nucleotide
Describe Bacteria DNA replication - Answer * Single circular chromosome with
one origin of replication
* No histones
* Helicase is a single enzyme
* DNA polymerase III is the main polymerase
Describe Archaea DNA replication - Answer * Single circular chromosome (can
have multiple copies); # of origins of replication varies
* Sometimes has histones
* Helicase is a complex of multiple proteins working together
with 100% Correct Answers| Latest Update
2024| Verified
What is DNA replication? - Answer * semi conservative
* The parent cell undergoes binary fission & there's 2 daughter cells
- Each daughter cell gets 1 strand from the parent & the other from DNA
replication
What does the origin of replication have? - Answer the DNAa sequence DNA
can bind to
Replication fork - Answer * where all the proteins for DNA replication are at
- As the replisomes moves down the DNA strand & unwinds it, the area where
nucleosomes are added is the replication fork
How many DNA poly III are at the replication fork? - Answer * multiple
What does the continuous (leading strand) have? - Answer * will have 1
DNA polymerase III
* moves 5' --> 3'
* starts with a phosphate
, What does the lagging strand have? - Answer * multiple DNA polymerase III
to synthesize the Okazaki fragments
* moves 3' --> 5'
* starts w/ a hydroxide
What is the daughter strand in relation to the parent strand? - Answer * opposite
What are the steps of Bacteria DNA replication proofreading? - Answer 1. DNA
polymerase makes an error & inserts the wrong nucleotide at the 3 prime end
of the strand being synthesized
2. The incorrect nucleotide separates from the template strand due to failure
to hydrogen bond properly. This causes the DNA POLY TO STOP
3. The 3 prime to 5 prime exonuclease activity of the DNA poly removes the
incorrect nucleotide
4. DNA poly restarts DNA synthesis, this time adding the correct nucleotide
Describe Bacteria DNA replication - Answer * Single circular chromosome with
one origin of replication
* No histones
* Helicase is a single enzyme
* DNA polymerase III is the main polymerase
Describe Archaea DNA replication - Answer * Single circular chromosome (can
have multiple copies); # of origins of replication varies
* Sometimes has histones
* Helicase is a complex of multiple proteins working together
