MLT 106 Unit 4 Practice Questions and Answers | 100% Pass

MLT 106 Unit 4 Practice Questions and Answers | 100% Pass What is the technique in which one excludes antibodies that could not be responsible for reactivity seen? What is advised? - Answer️️ -rule out technique only for homozygous expression What type of manufactured cells are used in panel evaluation? What phases are tested? - Answer️️ -O cells in 3 phases of testing (IS, 37, AHG) What is RESt? - Answer️️ -rabbit erythrocyte stroma; removes insignificant antibodies If phenotyping is difficult, what may be performed? - Answer️️ -homologous adsorption What is AHG? What is the difference between mono- and polyspecific? - Answer️️ - antihuman globulin; green bottle reagent mono has specific complement or anti-IgG poly contains several What technology produces monoclonal antibodies? - Answer️️ -hybridoma - mouse spleen lymphocyte with myeloma What produces polyclonal antibodies? - Answer️️ -rabbits; inject animals and harvest antibodies What is the difference between IAT and DAT? - Answer️️ -in vitro - in a tube in vivo - in the body (no incubation phase) Why would you run a DAT? - Answer️️ -transfusion within 2 weeks ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 2 history of antibodies pregnancy Do you use a DAT or IAT for cord blood studies in mother and neonate compatibility? - Answer️️ -DAT For what patients would you run an antibody evaluation? - Answer️️ -patients with a history of clinically significant antibodies How do you confirm validity of antibody identification? - Answer️️ -Phenotype Which method checks to see if the pattern of antigen-positive cells matches the pattern of reactivity? - Answer️️ -Inclusion Which method is based on negative results to rule out antibodies? - Answer️️ - Exclusion Does adsorption, elution, or neutralization use the individual's own cells due to presence of an interfering antibody when trying to find unexpected antibodies? - Answer️️ - Adsorption If a person is anemic and their own cells cannot be used, what do you do? - Answer️️ - homologous adsorption If you cannot use their own cells because they had a recent transfusion and have a positive DAT, what do you do? - Answer️️ -differential adsorption What is the purpose of elution? - Answer️️ -free bound antibodies from the RBC Which elution destroys the RBC? Which leaves the RBC intact - Answer️️ -total partial What temperature is gentle and total temperature induced elution? - Answer️️ -45 ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 3 56 What pH is necessary for pH elution? - Answer️️ -3 pH elution is good at identifying what type of antibodies? - Answer️️ -Non-ABO Which type of antigens are on Type 1 precursors? - Answer️️ -Lewis Is Lea or Leb produced in the absence of a secretor gene? - Answer️️ -Lea What causes Le(a+b+)? - Answer️️ -weak secretor gene What Le phenotype is observed when Lewis and secretor are both present? - Answer️️ -Le(a-b+) What Le phenotype is observed when Lewis is present and secretor is absent? - Answer️️ -Le(a+b-) What Le phenotype is observed when Lewis is absent and secretor is present? - Answer️️ -Le(a-b-) ABO antigens on body fluids When are Lewis antigens detectable in plasmas? - Answer️️ -After 10 days How long does it take for true Lewis phenotype to appear? - Answer️️ -6 to 7 years Which Lewis antigen reacts with both Lea and Leb and with 90% cord RBCs