Aseptic Technique Test Questions
and Answers All Correct
What tools are typically used for transferring small amounts of bacteria from one culture
medium to another - Answer-inoculating needle
inoculating loop
The inoculating loop should be placed in the hottest part of the flamw - Answer-until the
loop is red hot
What is the correct way to open an agar plate while removing a sample or inoculating
the plate - Answer-open one side of the lid diagonally off the plate
What is used in the lab to support the growth of microbial population - Answer-culture
medium
When should the inoculating loop be sterilized in the bunsen burner - Answer-before the
inoculum is picked up the culture rube
after the transfer is complete
How is a glass culture tube handled during aspetic transfers - Answer-the mouth is
passed through the flame right when opened an just before closing
Just by looking at a broth after transfer, one can tell if contamination has occured -
Answer-FALSE
Can you be certain you made a successful transfer if your sterile broth is turbid after
incubation - Answer-no, the broth will be turbid if it is correctly inoculated, but also if it
was contaminated during the inoculation procedure.
A glass tube of solid medium with an angled surface is referred to as a - Answer-slant
Which of these tubes shows the results of a correct slant to slant transfer? - Answer-
slant A ( has red in it)
Which of the following would indicate that contamination had occurred in a transfer from
an E.coli slant to a sterile slants. - Answer-there is red and white growth on the surface
of the slant.
The cloudy growth seen in a broth culture is referred to as the ____ of the culture. -
Answer-turbidity
and Answers All Correct
What tools are typically used for transferring small amounts of bacteria from one culture
medium to another - Answer-inoculating needle
inoculating loop
The inoculating loop should be placed in the hottest part of the flamw - Answer-until the
loop is red hot
What is the correct way to open an agar plate while removing a sample or inoculating
the plate - Answer-open one side of the lid diagonally off the plate
What is used in the lab to support the growth of microbial population - Answer-culture
medium
When should the inoculating loop be sterilized in the bunsen burner - Answer-before the
inoculum is picked up the culture rube
after the transfer is complete
How is a glass culture tube handled during aspetic transfers - Answer-the mouth is
passed through the flame right when opened an just before closing
Just by looking at a broth after transfer, one can tell if contamination has occured -
Answer-FALSE
Can you be certain you made a successful transfer if your sterile broth is turbid after
incubation - Answer-no, the broth will be turbid if it is correctly inoculated, but also if it
was contaminated during the inoculation procedure.
A glass tube of solid medium with an angled surface is referred to as a - Answer-slant
Which of these tubes shows the results of a correct slant to slant transfer? - Answer-
slant A ( has red in it)
Which of the following would indicate that contamination had occurred in a transfer from
an E.coli slant to a sterile slants. - Answer-there is red and white growth on the surface
of the slant.
The cloudy growth seen in a broth culture is referred to as the ____ of the culture. -
Answer-turbidity
