MIP 302 Final Exam Prep Questions with
Verified Answers
How long do you incubate blood Agar? - ANSWER 24 hours
How long do you incubate the indole test? - ANSWER 48 hours
How long do you incubate a KIA slant? - ANSWER 24 hours
How long do you incubate an LDC or ODC broth? - ANSWER 48 hours
How long do you incubate MacConkey Agars? - ANSWER 24 hours
How long do you incubate Methyl Red tests? - ANSWER 48 hours
How long do you incubate Nitrate broth? - ANSWER 48 hours
How long do you incubate PR Carbohydrate broths? - ANSWER 24-30 hours
How long do you incubate Pseudomonas Agar F? - ANSWER 18-24 hours
How long do you incubate Pseudosol Agar? - ANSWER 18-24 hours
How long do you incubate Simmons citrate agars? - ANSWER 48 hours
How long do you incubate Urea Slants? - ANSWER 48 hours
, How long do you incubate VP tests? - ANSWER 48 hours
Special procedures in KIA slant? - ANSWER Stab butt of the tube and streak the slant
Special procedures for ODC and LDC broths? - ANSWER Overlay with mineral oil
Special procedures for nitrate broth? - ANSWER Add Nitrate A and B and if no color
change add zinc
Special procedures for Oxidase test? - ANSWER Put disks with impregnanted reagents
onto slide then add water and bacteria
Special proceudres for PR carb broth? - ANSWER Add durham tube to broth to catch any
gas bacteria
Special procedures for Simmons citrate agar? - ANSWER Leave lid loose!
Positive result for blood agar test - ANSWER Beta- Complete lysis (clear zone)
Alpha- Incomplete (greenish brown)
Negative Result for blood agar test - ANSWER Gamma- no hemolysis (growth)
Positive result for catalase test - ANSWER Bubble
Negative result for Catalase test - ANSWER No bubbles
Verified Answers
How long do you incubate blood Agar? - ANSWER 24 hours
How long do you incubate the indole test? - ANSWER 48 hours
How long do you incubate a KIA slant? - ANSWER 24 hours
How long do you incubate an LDC or ODC broth? - ANSWER 48 hours
How long do you incubate MacConkey Agars? - ANSWER 24 hours
How long do you incubate Methyl Red tests? - ANSWER 48 hours
How long do you incubate Nitrate broth? - ANSWER 48 hours
How long do you incubate PR Carbohydrate broths? - ANSWER 24-30 hours
How long do you incubate Pseudomonas Agar F? - ANSWER 18-24 hours
How long do you incubate Pseudosol Agar? - ANSWER 18-24 hours
How long do you incubate Simmons citrate agars? - ANSWER 48 hours
How long do you incubate Urea Slants? - ANSWER 48 hours
, How long do you incubate VP tests? - ANSWER 48 hours
Special procedures in KIA slant? - ANSWER Stab butt of the tube and streak the slant
Special procedures for ODC and LDC broths? - ANSWER Overlay with mineral oil
Special procedures for nitrate broth? - ANSWER Add Nitrate A and B and if no color
change add zinc
Special procedures for Oxidase test? - ANSWER Put disks with impregnanted reagents
onto slide then add water and bacteria
Special proceudres for PR carb broth? - ANSWER Add durham tube to broth to catch any
gas bacteria
Special procedures for Simmons citrate agar? - ANSWER Leave lid loose!
Positive result for blood agar test - ANSWER Beta- Complete lysis (clear zone)
Alpha- Incomplete (greenish brown)
Negative Result for blood agar test - ANSWER Gamma- no hemolysis (growth)
Positive result for catalase test - ANSWER Bubble
Negative result for Catalase test - ANSWER No bubbles