Advanced ABO Practice Test Questions and Graded A+ Answers

A
VI
TU
IS
M

Advanced ABO Practice
O


Test Questions and
A
N




Graded A+ Answers
JP

, Forward Type - ANS Looking for unknown antigen on RBC, use known antibody
(Antisera), Agglutination indicates the presence of antigen, determines the ABO group

Reverse Type - ANS Use Known antigen (reagent red cells) to detect--Unknown
antibody in plasma, agglutination indicates the presence of antibody, confirms the ABO
group (most of the time, ~2% discrepancy)

ABO subgroups: "A" main subgroups - ANS Type "A" may be broken down into 2 main
subgroups: A1 and A2




A
What % of A people have an unexpected reverse reaction that doesn't match forward




VI
reaction - ANS 1% of the 40% that are type A

A1 phenotype has which antigens - ANS A1 and A (H)




TU
A2 phenotype has which antigens - ANS A only (NO such thing as A2 antigen) (H)

Quantitative A1 v. A2: A1 - ANS A1 has higher concentration of A (in addition to
having A1 antigen)
IS
Quantitative: A1 v A2: A2 - ANS A2 has fewer A antigens and Higher concentration of
H
M

Qualitative: A1 v A2 - ANS Linear (A) vs. branched (A1) forms of the same antigen
O


Group AB can also be subdivided into different subgroups - ANS A1B...A1, A, B, (H)
antigens. A2B.....A and B, (H) antigens
A



Order of ABO with decreasing amount of H - ANS O>A2>B>A2B>A1>A1B (usually
N




still have enough for 3+/4+ rxn.)
JP




A1 phenotype possible genotype - ANS A1/A1, A1/O, A1/A2

A2 phenotype possible genotype - ANS A2/A2, A2/O

B phenotype possible genotype - ANS B/B, B/O

A1B phenotype possible genotype - ANS A1/B

A2B phenotype possible genotype - ANS A2/B