©SOPHIABENNETT@2024-2025 Monday, August 26, 2024 6:09 AM
Thompson Rivers University (TRU)
PhysioEx Exercise 8 Study Guide with Complete Solutions
Circle the correct underlined term: The liver / stomach produces pepsin
which, in the presence of hydrochloric acid, digests protein. - Answer✔️✔️-
stomach
________________ is a hydrolytic enzyme that breaks starch down to
maltose. - Answer✔️✔️-Salivary amylase
A ________________ is made in order to compare a known standard to an
experimental standard. - Answer✔️✔️-Control
Circle True or False: A positive IKI test for starch will yield a bright orange
to green color - Answer✔️✔️-False
An enzyme has a pocket or ________________ , which the substrate or
substrates must fit into temporarily in order for catalysis to occur. -
Answer✔️✔️-active site
During digestion, the chief cells of the stomach secrete _____________ ,
which is responsible for the digestion of protein. - Answer✔️✔️-pepsin
Circle the correct underlined term: Positive / Negative controls are used
to determine whether there are any contaminating substances in the
reagents used in an experiment. - Answer✔️✔️-Negative
1
, ©SOPHIABENNETT@2024-2025 Monday, August 26, 2024 6:09 AM
Thompson Rivers University (TRU)
Circle True or False: At room temperature, both fats and oils are liquid and
are soluble in water. - Answer✔️✔️-false
A solution containing fatty acids formed by lipase activity will have a
________________ than a solution without such fatty acid production -
Answer✔️✔️-lower pH
What effect do you think boiling and freezing will have on the activity of
the amylase enzyme? - Answer✔️✔️-Boiling will decrease amylase activity
and freezing will have no effect.
Describe the effect that boiling had on the activity of
amylase. Why did boiling have this effect? How does the effect of freezing
differ from the effect of boiling? - Answer✔️✔️-By boiling the enzyme
activity stops; because of the high temperatures killing the enzymes
(denatured). Freezing does not change the structure of the enzyme so it
doesn't have an effect on the activity.
What is the purpose for including tube 3 and what can you conclude from
the result? - Answer✔️✔️-To see the comparison between boiling or freezing
before incubation and straight incubation with pH 7.0 buffer, 70oC, for
60minutes.
Describe how you determined the optimal pH for amylase activity. -
Answer✔️✔️-At pH 7 the highest activity of salivary amylase occurred. The
2
Thompson Rivers University (TRU)
PhysioEx Exercise 8 Study Guide with Complete Solutions
Circle the correct underlined term: The liver / stomach produces pepsin
which, in the presence of hydrochloric acid, digests protein. - Answer✔️✔️-
stomach
________________ is a hydrolytic enzyme that breaks starch down to
maltose. - Answer✔️✔️-Salivary amylase
A ________________ is made in order to compare a known standard to an
experimental standard. - Answer✔️✔️-Control
Circle True or False: A positive IKI test for starch will yield a bright orange
to green color - Answer✔️✔️-False
An enzyme has a pocket or ________________ , which the substrate or
substrates must fit into temporarily in order for catalysis to occur. -
Answer✔️✔️-active site
During digestion, the chief cells of the stomach secrete _____________ ,
which is responsible for the digestion of protein. - Answer✔️✔️-pepsin
Circle the correct underlined term: Positive / Negative controls are used
to determine whether there are any contaminating substances in the
reagents used in an experiment. - Answer✔️✔️-Negative
1
, ©SOPHIABENNETT@2024-2025 Monday, August 26, 2024 6:09 AM
Thompson Rivers University (TRU)
Circle True or False: At room temperature, both fats and oils are liquid and
are soluble in water. - Answer✔️✔️-false
A solution containing fatty acids formed by lipase activity will have a
________________ than a solution without such fatty acid production -
Answer✔️✔️-lower pH
What effect do you think boiling and freezing will have on the activity of
the amylase enzyme? - Answer✔️✔️-Boiling will decrease amylase activity
and freezing will have no effect.
Describe the effect that boiling had on the activity of
amylase. Why did boiling have this effect? How does the effect of freezing
differ from the effect of boiling? - Answer✔️✔️-By boiling the enzyme
activity stops; because of the high temperatures killing the enzymes
(denatured). Freezing does not change the structure of the enzyme so it
doesn't have an effect on the activity.
What is the purpose for including tube 3 and what can you conclude from
the result? - Answer✔️✔️-To see the comparison between boiling or freezing
before incubation and straight incubation with pH 7.0 buffer, 70oC, for
60minutes.
Describe how you determined the optimal pH for amylase activity. -
Answer✔️✔️-At pH 7 the highest activity of salivary amylase occurred. The
2
