lOMoARcPSD|28593260
Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Lab 7 for
Microbiology Lab
from
Straighterline
Latest Update
2024/2025
Graded A+
, lOMoARcPSD|28593260
Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Student Name:
Access Code (located on the underside of the lid of your lab kit):
C321-MNHU
“Pre-Lab Questions”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines,
and which are pyrimidines?
Adenine pairs with Thymine and Cytosine pairs with Guanine. Adenine and Guanine are
both purines and Thymine and Cytosine are pyrimidines.
2. How is DNA information used to make proteins? What are the steps of this
process?
DNA information is used to make proteins through transcription and translation. The first
step is when the enzymes from the DNA molecules are transcribed into mRNA. The
mRNA goes to the ribosome creating tRNA and is translated into amino acids that block
proteins.
3. Give an example of a scenario in which you would perform PCR vs a scenario
in which you would use recombinant DNA technology.
A scenario when I would need to perform PCR is when determining the source of a
disease or illness and collecting DNA from blood samples. When you’d have to use
recombinant DNA technology would be when needing to take human growth hormones.
4. What occurs during each of the three steps involved in the PCR cycle? How
has the use of PCR changed biotechnology?
The three steps involved in the PCR cycle are denaturing, annealing, and elongation.
Denaturing is when the DNA is heated up and separates into 2 single strands. Annealing
is the next step, temperatures cool down and the DNA primers attach themselves to the
DNA template. In elongation, the temperature goes back up high and the new strand of
Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Lab 7 for
Microbiology Lab
from
Straighterline
Latest Update
2024/2025
Graded A+
, lOMoARcPSD|28593260
Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Student Name:
Access Code (located on the underside of the lid of your lab kit):
C321-MNHU
“Pre-Lab Questions”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines,
and which are pyrimidines?
Adenine pairs with Thymine and Cytosine pairs with Guanine. Adenine and Guanine are
both purines and Thymine and Cytosine are pyrimidines.
2. How is DNA information used to make proteins? What are the steps of this
process?
DNA information is used to make proteins through transcription and translation. The first
step is when the enzymes from the DNA molecules are transcribed into mRNA. The
mRNA goes to the ribosome creating tRNA and is translated into amino acids that block
proteins.
3. Give an example of a scenario in which you would perform PCR vs a scenario
in which you would use recombinant DNA technology.
A scenario when I would need to perform PCR is when determining the source of a
disease or illness and collecting DNA from blood samples. When you’d have to use
recombinant DNA technology would be when needing to take human growth hormones.
4. What occurs during each of the three steps involved in the PCR cycle? How
has the use of PCR changed biotechnology?
The three steps involved in the PCR cycle are denaturing, annealing, and elongation.
Denaturing is when the DNA is heated up and separates into 2 single strands. Annealing
is the next step, temperatures cool down and the DNA primers attach themselves to the
DNA template. In elongation, the temperature goes back up high and the new strand of
