Ammara Aslam
P180267
Unit 6: Investigative Project
In this assignment I will discuss how my experiment went and what went to plan and what
did not go to plan.
My method for the experiment:
1. I will add two drops of iodine solution into each depression of two spotting tiles
2. I then will measure 2 cm3 of amylase enzyme solution using a 10ml
measuring cylinder and add it to a test tube
3. I then will measure 2 cm3 of starch solution using a 10ml measuring cylinder
and add it into the same tube.
4. Then i'll measure and add 1 cm3 of pH solution to the test tube
5. Mix the solution in the test tube with a glass rod and place it into the test tube
rack in the water bath
6. Using a pipette I will remove a few drops of solution every 20 seconds from
the test tube and put them into a different well of the spotting tile
7. Repeat until the iodine solution stops turning black
8. Record the time this takes
9. Repeat with different pH solutions”
Results table:
pH Time taken (min) Time taken (min) Time taken (min)
1 2 3
2 After 15 minutes no After 10 minutes no After 10 minutes no
reaction result result
4 6.30 7 7
6 2 2 2
8 6 6.30 6
10 After 10 minutes no After 10 minutes no After 10 minutes no
result result result
1
, Ammara Aslam
P180267
The reason as to why I counted my results for 30 second intervals was because this was the
most appropriate interval timings in my opinion and most people who have done this
experiment tend to agree. Because if I did any longer than 30 seconds it may have cause
results to be inaccurate as it may be too far from the actual time which the reaction occurred,
or if i used intervals which were too short such as every 10 seconds it would be very time
consuming and would require a lot of work and it would be likely to miss out an interval
meaning you are not being consistent.
After carrying out my experiment I analysed my results and worked out averages I then
plotted my average results onto a graph:
2
P180267
Unit 6: Investigative Project
In this assignment I will discuss how my experiment went and what went to plan and what
did not go to plan.
My method for the experiment:
1. I will add two drops of iodine solution into each depression of two spotting tiles
2. I then will measure 2 cm3 of amylase enzyme solution using a 10ml
measuring cylinder and add it to a test tube
3. I then will measure 2 cm3 of starch solution using a 10ml measuring cylinder
and add it into the same tube.
4. Then i'll measure and add 1 cm3 of pH solution to the test tube
5. Mix the solution in the test tube with a glass rod and place it into the test tube
rack in the water bath
6. Using a pipette I will remove a few drops of solution every 20 seconds from
the test tube and put them into a different well of the spotting tile
7. Repeat until the iodine solution stops turning black
8. Record the time this takes
9. Repeat with different pH solutions”
Results table:
pH Time taken (min) Time taken (min) Time taken (min)
1 2 3
2 After 15 minutes no After 10 minutes no After 10 minutes no
reaction result result
4 6.30 7 7
6 2 2 2
8 6 6.30 6
10 After 10 minutes no After 10 minutes no After 10 minutes no
result result result
1
, Ammara Aslam
P180267
The reason as to why I counted my results for 30 second intervals was because this was the
most appropriate interval timings in my opinion and most people who have done this
experiment tend to agree. Because if I did any longer than 30 seconds it may have cause
results to be inaccurate as it may be too far from the actual time which the reaction occurred,
or if i used intervals which were too short such as every 10 seconds it would be very time
consuming and would require a lot of work and it would be likely to miss out an interval
meaning you are not being consistent.
After carrying out my experiment I analysed my results and worked out averages I then
plotted my average results onto a graph:
2
