(MB) ASCP Study Exam Questions
What is metagenome? correct answersSampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? correct answersUGT1A1
NGS related to deletions and insertions, coverage, depth? correct answers-Paired-end reads for
detection of indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand? correct answersEuk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand? correct answersEuk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP? correct answers-Loop-mediated Isothermal Amplification utilizes Bst
Polymerase
-Has helicase activity and activity at annealing/elongating temps
Highest resistance gene in enterococcus? correct answersvanA has the highest resistance in
enterococcus
Resistance in mecA gene in MRSA codes for? correct answersmecA responsible for antibiotic resistance
in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like
penicillin.
Important in gene expression, defense in eukaryotes? correct answersPerson guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.
,Donor/Recipient - what's informative loci? correct answersNo matching alleles is informative, you can
tell D from R; non-informative would be both D&R matching.
Bond between DNA and histones? correct answersIonic - DNA is negative and histones are positive
Amino Acids that make up histones? correct answersArginine and Lysine - positively charged amino acids
Multiplex modifications for low yield? correct answersPerson guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting correct answersIncrease Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
Remove Mg2+ --> Increase Stringency
Less Time --> Increase Stringency
Add Formamide --> Lowers Stringency
Add Bovine Serum --> Lowers Stringency
Increase Probe Conc. --> Lowers Stringency
How does an amplification control help? correct answersDifferentiates a true negative from a false
negative
Amplification controls have a target that should always amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? correct answersCF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
, What to use FISH for: correct answersPWS/AS can be detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
How often should a competency be given/done for someone in a complex lab? correct answersAnnually
Important in Final Lab Report in CLIA? correct answersLab director signature?
Neurotoxin in gel components? correct answersEthidium Bromide
What phenomenon is observed in EtBr in gel electrophoresis? correct answersProducts slowed because
EtBr alters DNA shape, charge, flexibility
Heteroduplex correct answersdsDNA where the complement strands are not completely complementary
MALDI question something about how it works? correct answersMatrix-assisted laser
desorption/ionization, blasts a surface with sample to produce positive ions that can be detected.
MALDI-Tof question? correct answersSame as MALDI, but used time of flight (TOF) to differentiate
between ions. Lighter ions travel faster.
NGS reaction order? correct answersFragmentation,
End Repair,
Adapter Ligation
Amplification
Sequencing
HIV processing order? correct answersPlasma separation, Extraction, RNA Modification, Amplification
What is metagenome? correct answersSampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? correct answersUGT1A1
NGS related to deletions and insertions, coverage, depth? correct answers-Paired-end reads for
detection of indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand? correct answersEuk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand? correct answersEuk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP? correct answers-Loop-mediated Isothermal Amplification utilizes Bst
Polymerase
-Has helicase activity and activity at annealing/elongating temps
Highest resistance gene in enterococcus? correct answersvanA has the highest resistance in
enterococcus
Resistance in mecA gene in MRSA codes for? correct answersmecA responsible for antibiotic resistance
in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like
penicillin.
Important in gene expression, defense in eukaryotes? correct answersPerson guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.
,Donor/Recipient - what's informative loci? correct answersNo matching alleles is informative, you can
tell D from R; non-informative would be both D&R matching.
Bond between DNA and histones? correct answersIonic - DNA is negative and histones are positive
Amino Acids that make up histones? correct answersArginine and Lysine - positively charged amino acids
Multiplex modifications for low yield? correct answersPerson guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting correct answersIncrease Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
Remove Mg2+ --> Increase Stringency
Less Time --> Increase Stringency
Add Formamide --> Lowers Stringency
Add Bovine Serum --> Lowers Stringency
Increase Probe Conc. --> Lowers Stringency
How does an amplification control help? correct answersDifferentiates a true negative from a false
negative
Amplification controls have a target that should always amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? correct answersCF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
, What to use FISH for: correct answersPWS/AS can be detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
How often should a competency be given/done for someone in a complex lab? correct answersAnnually
Important in Final Lab Report in CLIA? correct answersLab director signature?
Neurotoxin in gel components? correct answersEthidium Bromide
What phenomenon is observed in EtBr in gel electrophoresis? correct answersProducts slowed because
EtBr alters DNA shape, charge, flexibility
Heteroduplex correct answersdsDNA where the complement strands are not completely complementary
MALDI question something about how it works? correct answersMatrix-assisted laser
desorption/ionization, blasts a surface with sample to produce positive ions that can be detected.
MALDI-Tof question? correct answersSame as MALDI, but used time of flight (TOF) to differentiate
between ions. Lighter ions travel faster.
NGS reaction order? correct answersFragmentation,
End Repair,
Adapter Ligation
Amplification
Sequencing
HIV processing order? correct answersPlasma separation, Extraction, RNA Modification, Amplification
