Microbiology BOC
Questions and Answers
The proper blood to broth ratio for blood cultures to reduce the antibacterial effect of
serum in adults is:
✓~ 1:10
the most appropriate method for collecting a urine specimen from a patient with an
indwelling catheter?
✓~ aspirate urine aseptically from the catheter tubing
which of the following groups of specimens would be acceptable for anaerobic
culture?
✓~ pleural fluid, brain abcess fluid
sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood cultures
because it:
✓~ inactivates neutrophils and components of serum complement
the optimal collection of a wound specimen for culture of anaerobic organisms is a:
,✓~ syringe filled with pus, obtained before administration of antibiotics
the most important variable in the recovery of organisms in adult patients with
bacteremia (bacterial sepsis) is:
✓~ the recommended volume of blood ordered
virus transport medium should contain agents that:
✓~ inhibit bacterial and fungal growth
a bronchoscopy sample with the request culture for Legionella is sent to the lab. the
correct plating protocol is:
✓~ culture on buffered charcoal yeast extract agar with antibiotics
a community hospital microbiology lab is processing significant numbers of stool
cultures because of an outbreak of diarrhea following heavy rains and flooding in the
county. a media that should be incorporated in the plating protocol is:
✓~ thiosulfate citrate bile salts sucrose for Vibrio spp.
a male urethral discharge specimen submitted for culture should be inoculated to:
✓~ chocolate and modified Thayer-Martin agars
the steam autoclave method of sterilization requires
,✓~ 15# pressure 15 minutes
Which selective medium is used for the isolation of gram-positive microorganisms?
✓~ Columbia CNA with 5% sheep blood
Campylobacter jejuni isolation requires the fecal specimen be:
✓~ inoculated onto selective plating media and incubated in reduced oxygen with added
CO2 at 42C
which of the following specimen requests is acceptable?
✓~ urine culture for acid-fast bacilli
a CSF containing only 2 drops of CSF was collected by a lumbar puncture from a 25
year old male, and submitted for stat gram stain and culture. The direct specimen
gram stain was reported as many neutrophils and no microorganisms seen. The
remaining drop of CSF should be inoculated to:
✓~ chocolate agar
a diabetic food swab from an 82 year old woman with recurrent infections is
submitted for culture. The gram stain reveals:
Many neutrophils no squamous cells
many gram-neg bacilli
, Many gram-pos cocci in chains
Dr requests that all pathogens be worked up, in addition to sheep blood, chocolate
and MacConkey, the tech may also process a:
✓~ CNA agar plate
which of the following is the most appropriate specimen source and primary media
selection?
✓~ endocervical; chocolate, Martin Lewis
which of the following is the most appropriate organism and media combo?
✓~ Yersinia enterocolitica-CIN agar
a gram stain from a hand wound swab:
moderate neutrophils, no squamous, moderat GP cocci in clusters, moderate GN
bacilli
the appropriate media that will selectively isolate each org?
✓~ CNA, MacConkey
an organism that must be incubated in a microaerophilic environment for optimal
recovery is:
✓~ C. jejuni
Questions and Answers
The proper blood to broth ratio for blood cultures to reduce the antibacterial effect of
serum in adults is:
✓~ 1:10
the most appropriate method for collecting a urine specimen from a patient with an
indwelling catheter?
✓~ aspirate urine aseptically from the catheter tubing
which of the following groups of specimens would be acceptable for anaerobic
culture?
✓~ pleural fluid, brain abcess fluid
sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood cultures
because it:
✓~ inactivates neutrophils and components of serum complement
the optimal collection of a wound specimen for culture of anaerobic organisms is a:
,✓~ syringe filled with pus, obtained before administration of antibiotics
the most important variable in the recovery of organisms in adult patients with
bacteremia (bacterial sepsis) is:
✓~ the recommended volume of blood ordered
virus transport medium should contain agents that:
✓~ inhibit bacterial and fungal growth
a bronchoscopy sample with the request culture for Legionella is sent to the lab. the
correct plating protocol is:
✓~ culture on buffered charcoal yeast extract agar with antibiotics
a community hospital microbiology lab is processing significant numbers of stool
cultures because of an outbreak of diarrhea following heavy rains and flooding in the
county. a media that should be incorporated in the plating protocol is:
✓~ thiosulfate citrate bile salts sucrose for Vibrio spp.
a male urethral discharge specimen submitted for culture should be inoculated to:
✓~ chocolate and modified Thayer-Martin agars
the steam autoclave method of sterilization requires
,✓~ 15# pressure 15 minutes
Which selective medium is used for the isolation of gram-positive microorganisms?
✓~ Columbia CNA with 5% sheep blood
Campylobacter jejuni isolation requires the fecal specimen be:
✓~ inoculated onto selective plating media and incubated in reduced oxygen with added
CO2 at 42C
which of the following specimen requests is acceptable?
✓~ urine culture for acid-fast bacilli
a CSF containing only 2 drops of CSF was collected by a lumbar puncture from a 25
year old male, and submitted for stat gram stain and culture. The direct specimen
gram stain was reported as many neutrophils and no microorganisms seen. The
remaining drop of CSF should be inoculated to:
✓~ chocolate agar
a diabetic food swab from an 82 year old woman with recurrent infections is
submitted for culture. The gram stain reveals:
Many neutrophils no squamous cells
many gram-neg bacilli
, Many gram-pos cocci in chains
Dr requests that all pathogens be worked up, in addition to sheep blood, chocolate
and MacConkey, the tech may also process a:
✓~ CNA agar plate
which of the following is the most appropriate specimen source and primary media
selection?
✓~ endocervical; chocolate, Martin Lewis
which of the following is the most appropriate organism and media combo?
✓~ Yersinia enterocolitica-CIN agar
a gram stain from a hand wound swab:
moderate neutrophils, no squamous, moderat GP cocci in clusters, moderate GN
bacilli
the appropriate media that will selectively isolate each org?
✓~ CNA, MacConkey
an organism that must be incubated in a microaerophilic environment for optimal
recovery is:
✓~ C. jejuni
