MLPAO Test Prep ~ Hematology, 2024/5. 100%
What does CBC stand for?
Complete Blood Count
What are the formed elements of blood?
WBC, RBC, and Platelets
EDTA Anticoagulant
Maintains cell shape and size and prevents blood from clotting by binding to calcium
Heparin Anticoagulant
Initiates cell clumping and provides blue background staining
Citrate Anticoagulant
Dilutes the blood due to the volume of the liquid and is used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing
4
CBC handling and storage: When transporting to other labs/hospitals
surround with ice packs
CBC handling and storage: Samples are _____ before testing
mixed well by inversion
CBC handling and storage: Samples must be tested within __
24h
CBC handling and storage: Peripheral blood smears must be made within
24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR TESTING
24h
What happens if CBC is tested after 24h?
Platelets clump, WBC nuclei disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs
swell and break down
Coagulation: Specimen of choice?
Plasma
, Coagulation: What colour tube? What is the additive?
light blue, sodium citrate
Sodium Citrate binds to ___ preventing ____
calcium, coagulation
Sodium Citrate tube must be
filled to the maximum draw capacity
Coagulation: Document if patient on
anticoagulant therapy or aspirin
Handling and Storage of Coagulation samples: Preparation requirements
Centrifuge and separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival
Freeze, 4h
Coagulation samples: Some clotting factors are considered to be
highly labile (destroyed at room temperature)
What are the three methods of Peripheral blood smears (PBS)?
Wedge method (manual), Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide ___
Decreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered.
First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide ___
Increased
HCT Values: Male, Female
0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide
2/3, ¾
Good blood film should have a slightly ___ or ____ edge
Rounded, feathered
The angle of the spreader slide is approximately ___ degrees
What does CBC stand for?
Complete Blood Count
What are the formed elements of blood?
WBC, RBC, and Platelets
EDTA Anticoagulant
Maintains cell shape and size and prevents blood from clotting by binding to calcium
Heparin Anticoagulant
Initiates cell clumping and provides blue background staining
Citrate Anticoagulant
Dilutes the blood due to the volume of the liquid and is used for platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing
4
CBC handling and storage: When transporting to other labs/hospitals
surround with ice packs
CBC handling and storage: Samples are _____ before testing
mixed well by inversion
CBC handling and storage: Samples must be tested within __
24h
CBC handling and storage: Peripheral blood smears must be made within
24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR TESTING
24h
What happens if CBC is tested after 24h?
Platelets clump, WBC nuclei disintegrate, WBC cytoplasm contain dark granules and Vacuoles, and RBCs
swell and break down
Coagulation: Specimen of choice?
Plasma
, Coagulation: What colour tube? What is the additive?
light blue, sodium citrate
Sodium Citrate binds to ___ preventing ____
calcium, coagulation
Sodium Citrate tube must be
filled to the maximum draw capacity
Coagulation: Document if patient on
anticoagulant therapy or aspirin
Handling and Storage of Coagulation samples: Preparation requirements
Centrifuge and separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival
Freeze, 4h
Coagulation samples: Some clotting factors are considered to be
highly labile (destroyed at room temperature)
What are the three methods of Peripheral blood smears (PBS)?
Wedge method (manual), Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide ___
Decreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered.
First
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide ___
Increased
HCT Values: Male, Female
0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide
2/3, ¾
Good blood film should have a slightly ___ or ____ edge
Rounded, feathered
The angle of the spreader slide is approximately ___ degrees
